基因的

Infiltration of the genes of one species into the gene pool of another through repeated backcrossing of an interspecific hybrid with one of its parents.

基因渗入一物种的基因通过不断地与另一物种基因的亲代种间逆向杂交，而渗透进另一物种基因库的过程

Methods A PCR-based RFLP procedure was employed to detect the alleles and genotypes of PPARγ gene in 111family trios including 333 subjects of Han descent population in the north of China.

采用PCR技术和限制性内切酶片段长度多态性的方法，检测中国北方汉族111个核心家系，共333个研究对象的PPARγ基因的等位基因和基因型。

The transcript of M-PAL gene in the leaves was increased when the plantlets of plantain were inoculated with Fusarium oxysporun f. sp.cubense, indicating that the expression of M-PAL may be related to resistance of banana Fusarium wilt.

对接种香蕉枯萎病菌4号生理小种Fusarium oxysporum f.sp.cubense(FOCrace4后大蕉叶片中M-PAL基因的转录谱进行研究表明，在接种枯萎病菌后，M-PAL基因在叶片中的转录水平提高，因此推测M-PAL基因的表达可能与香蕉枯萎病抗性相关。

Methods Ten mature Wistar rats were divided into normal control group (5 rats) and adenovirus (E1, E3-Deleted and carried math1 and enhanced green fluorescent protein report gene, Ad-Math1-EGFP) scala vestibuli transfer group (5 rats). Right ears of the Ad-Math1-EGFP transfering group rats were deliveried 5μl Ad-Math1-EGFP (physical tite 2.1×10^11v.p./ml) into cochleas through the way of drilling scala vestibuli of cochlear basal turn. As a control, the normal group received nothing to inner ear. In order to estimate functional condition of vestibule and cochlea, the click-evoked potentials on the surface of the cervical dura mater, auditory brain stem response and swimming time were recorded in all rats at 7 days after treatment, and then histologic and morphologic observation were carried out after animals were sacrificed. Results All animals' morphologic observation showed that inner ear hair cells were normal after transfer.

将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein， Ad-Math1-EGFP)前庭阶导入组，每组5只，实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×10^11v.p/ml的上述腺病毒5μl，对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater， CDM-CEP)、听性脑干反应阈值检测和游泳试验，评价前庭和耳蜗功能，然后将动物处死进行组织形态学观察。

A recombinant pl asmid (designated as p8.0) was obtaining by ligating these three fragments. The p8.0 was subcloned into a plasmid (designated as p8.2) containing entire LTR of EIAV DLA. The complete nucleotide sequence of DLA strain of EIAV was determined by sequencing the p 8.2 . The purified p8.2 was used to transfect donkey leuko cyte cultures.

将此8.0kb EIAV全基因再亚克隆到含有一完整EIAV DLA株长末端重复序列的质粒中，获得一含有EIAV驴白细胞弱毒前病毒全基因的重组质粒，将其命名为p8.2，经核苷酸序列分析，证明p8.2含有EIAV前病毒的全基因。

They are OsMADS1, OsMADS5, OsMADS7 (OsMADS45), OsMADS8 (OsMADS24) and OsMADS34, in which OsMADS1 is currently the best characterized, the others are not documented well. On the basis of the results from dicots, we investigated the E function genes in rice using In Situ Hybridization and RNAi technique.

水稻是单子叶植物的模式植物，水稻中至少有5个E类基因，分别是OsMADS1、OsMADS5、OsMADS7、OsMADS8和OsMADS34 ，其中除了对OsMADS1基因有较深入的研究外，对其它几个E类基因的功能了解甚少。

Objective To construct recombination eukaryon expression plasmid for human parathyroid hormone gene, assay PTH expression and biological activity after transfection in vitro and evaluate gene therapy effect on hypoparathyroidsm.

目的构建甲状旁腺激素基因的重组真核表达质粒，评价体外转染后PTH基因的表达与生物学活性，同时观察其对甲状旁腺功能减退动物的基因治疗作用。

The phytase gene was cloned in this study would not only provided excellent gene resource which could express in the microbe and plant but also remedied the vacancy of phytase gene cloned in the funguses.

本研究所克隆的植酸酶基因不仅为植酸酶进一步在各种微生物、植物中表达提供了优良的基因源，而且弥补了食用菌中克隆植酸酶基因的空白。

The cDNA of human μOR with a six consecutive histidines tag at its carboxyl terminus had been introduced in the baculovirus genome under the control of the polyhedrin promoter.

含目的基因HμOR-6His cDNA的基因片段被整合在转录多角体蛋白基因的强启动子控制下的杆状病毒基因组中。

After inserting the mutant fluorescence protein gene into the vector, the novel vaccinia virus vector p16H1FP and p16H2FP to express proteins as fusion products containing an additional amino-terminal stretch of six histidines were constructed.

我们用PCR扩出N-端融合6×His·Tag多克隆位点基因序列，插入p16表达载体，并在其多克隆位点下游装入带有巨细胞病毒启动子的突变型荧光蛋白基因，构建成两个读框的N-端融合6×His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。