基因的

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

It is shown that G gene has the highest speed during the evolution and has uniqueinquilinous body in order to escape immune pressure from inquilinous body byanalyzing phylogenic trees of five genes in the whole genome. In the evolution of Ggene, DRV and MRV are in different groups. DRV, Chinese vaccine isolated used inhuman and Japanese vaccine isolated used in human are in the same group. MRV,CVS and street viruses isolated from dogs are in the some group. It is shown thatDRV is vaccine isolated compared with phylogenic trees of other genes, and it istestified previous conclusion that unqualified vaccine used for human was used nearthe infected beer cote resulted in spotted deer infected.

对狂犬病毒基因组5个基因的分子进化树分析表明，为了逃避宿主的免疫压力，G基因的进化最快速，而且具有宿主的特异性，在G基因的进化中，DRV和MRV分别位于不同的组群，DRV与中国人用疫苗株和日本疫苗株在一个组群，MRV与CVS以及犬脑分离的街毒归在一个组群，结合其他基因的进化树表明，DRV与疫苗株具有较近的亲缘关系，从分子水平验证了以前对DRV流行病学调查得出的推测，在狂犬病疫情发生的鹿场附近曾使用过人用狂犬病疫苗，导致梅花鹿感染狂犬病毒。

Objective:To analyze the genotype of the porin gene in Neisseria gonorrhoea wild strains. Bacterial agglutination has been carried out to verify whether there is an antigenicity change caused by the porin gene mutation.

目的：采用PCR和探针技术分析临床淋病奈瑟菌野生株孔蛋白基因的基因型，并和相应多克隆抗体进行反应以检测基因突变对抗原性的影响。

The nucleotide sequence and the predicted protein sequence shared high sequence identity with other mammalian homologues.

比较猪RPLP0基因和人及小鼠同源基因的cDNA序列和蛋白质序列，结果表明该基因在3个物种中具有高的相似性。

Objective To construct the suicide gene herpes simplex virus thymidine kinase gene and to sequence its structure for identification.

目的利用分子生物学技术，构建胸苷激酶基因自杀基因并测定其结构，为该自杀基因的进一步研究提供良好的实验基础。

Objective To construct the suicide gene herpes simplex virus thymidine kinase gene and to sequence its structure for identification.

目的 利用分子生物学技术，构建胸苷激酶基因自杀基因并测定其结构，为该自杀基因的进一步研究提供良好的实验基础。

A co-regulation existed in the expression among chalcone synthase, methionine sulfoxide reductase and acyl carrier protein Ⅱ, also did in the expression between allergenic protein and prolamine.

查尔酮合酶、S-腺苷基甲硫氨酸还原酶和酰基载体蛋白Ⅱ的表达存在基因间共调控，过敏反应蛋白和醇溶蛋白基因的表达也存在基因间共调控。

The enzyme spectrum of FMR1 locus also indicates the potential existence of other gene in the region several kb 5'to FMR1 gene, whose inactivation may also be affective in Fra syndrome.

FMR1基因位点的酶谱特征也支持在该基因的上游几百kb范围内可能还有未知基因，其失活也与脆性X综合征有关。

In these two plant expression vectors, the expression of the DAS and DAK genes was under the control of tomato Rubisco-3c promoter. The Prbcs promoter contains a transit peptide sequence which allows the location of the expressed DAS and DAK into chloroplasts. The DAS and DAK genes were introduced into Nicotiana tabacum and Petunia hybrida by Agrobacterium tumefaciens- mediated procedure.

研究结果如下：(1)根据已克隆的C.boidinii酵母菌DAS和P.pastoris DAK基因的序列设计特异性引物，通过PCR分别从这两种酵母菌的基因组中扩增DAS、DAK基因，利用gateway技术构建DAS、DAK基因的植物表达载体pK2-Rbes-DAS、pn2-Rbcs-DAK，在构建好的植物表达载体中，DAS和DAK基因的表达都在番茄Rubisco小亚基rbcS-3C启动子的控制之下。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。