基因的

(1) It is a good way to establish the SD rat-PCP model by groin subcutaneous injection with dexamethasone sodium phosphate.(2) The Pc DHFR and DHPS genes of the sulfanilamide treatment group and positive control group are all amplified.

2实验组和阳性对照组Pc DHFR和DHPS基因的PCR扩增均呈阳性，两个基因与Gene bank内相应大鼠源肺孢子的基因序列同源性较高。

The cloned BBE gene sequences were 94.84% identified with the reported BBE genes in GenBank previously. Based on the cDNA sequences of COR and BBE ,two fragments about 400～500 bp from each gene with lower identity among them were cloned. The fusion gene BC (744 bp) is fused by the PCR technique. Then the promoter CaMV 35S driven, containing'forward BC fusion fragments-reverse pdk intron-reverse BC fusion fragments', plant siRNA expression vector were constructed based on the vectors pHANNIBAL and pART27.The work will lay the foundation for breeding a low morphine and high thebaine poppy.

利用blast及分子生物学软件DNAStar对COR和BBE基因的cDNA序列同源性进行分析比较，分别从各基因中筛选和克隆了一段同源性极低、约400～500 bp的片段；并应用重叠PCR法将其拼接成744 bp的融合基因BC，以中间载体pHANNIBAL和植物表达载体pART27为基础，构建了以CaMV 35S启动子驱动的含有"正向BC融合片段- pdk内含子-反向BC融合片段"的ihRNAi植物表达载体，通过转化野生罂粟，初步研究了以COR和BBE基因为靶标的RNAi对内源吗啡合成的抑制效果，为进一步培育低吗啡高蒂巴因的罂粟种质提供了依据。

In this experiment, His 5 gene was inserted into Ura 3 gene and constructed a new plasmid pLRH33, which contained a complete His 5 gene and partial Ura 3 gene at the ends of His 5 gene.

实验将His 5基因插入到一个Ura 3基因的中间，构建了一个新的质粒pLRH33，从而打断了Ura 3 基因使之不能表达。

The results indicated the origin of Nipponia Nippon was comparative singleness. Homology of eighteen Cyt b gene sequences in mtDNA region among Threskiornithidae, Ciconiidae, Phoenicopteridae, Ardeidae and Gruidae were analyzed and their BSR and genetic distances calculated.

将朱鹮mtDNA Cyt b基因序列与鹮科、鹳科、红鹳科、鷺科、鶴科共18个个体的mtDNA Cyt b基因进行同源序列分析，并计算了mtDNA Cyt b基因的碱基相似率和遗传距离。

1Livestock Research Institute, Council of Agriculture(2)Department of Animal Science, National Pingtung University of Science and TechnologyGenetic variation in the N-acetylglucosamine 6-sulfatase (G6S) gene is the key role in caprine Mucopolysaccharidosis IIID.

本研究建立山羊黏多醣症遗传缺陷之单股构型多态性基因型检测方法，应用此方法来检测乙醯醣胺氨基硫酸酶(N-acetylglucosamine 6-sulfatase， G6S)基因型不再需要使用限制酶，可节省检测时间、成本与人力。G6S基因的遗传变异在山羊黏多醣症第三型扮演重要的角色。

The first full-length cDNA of BA DH gene was cloned from spinach of the Chenopodiaceae. Then the gene was often taken as a contrast when BA DH genes from other plants were studied.

其中，BADH基因的全长cDNA序列首先被从藜科植物菠菜中克隆到，此后人们相继以该基因为参照，研究其它植物中的BADH基因。

The hypermethylation of 5 CpG in promoter region serves as a mechanism of the inactivation of RASSF1A gene. This mechanism has been widely confirmed in multiple tumorgenesis including prostate carcinoma cell line DU145. Object Our study is to disclose the epigenic mechanism of the carcinogenesis of prostate cancer. Including: 1. We use demethylation agents in combination with chemotherapeutic drugs, to disclose whether demethylation agents can enhance the anticancer effect of chemotherapeutic drugs. 2. to study whether demethylation drugs can affect the methylation level of RASSF1A gene in.

目的 本研究旨在探讨前列腺癌的表遗传学发生发展机制，包括： 1、甲基化抑制剂与多种化疗药物联合应用，探讨二者间的协同作用，增强化疗药物疗效； 2、体外应用甲基化抑制剂对前列腺癌细胞DU145的RASSF1A基因甲基化水平的影响； 3、体外应用甲基化抑制剂对前列腺癌细胞DU145的RASSF1A基因及蛋白表达的影响，以期为进一步深入探索通过改变抑癌基因的甲基化而为前列腺癌发生机制和基因治疗提供有意义的数据和理论依据。

An expression vector was constructed with a safe marker gene, 6-phosphomannose isomerase, and an insect-resistant gene, Galanthus nivalis agglutinin, was used in rice genetic transformation in the present study.

采用农杆菌介导法将来源于大肠杆菌的6-磷酸甘露糖异构酶安全选择标记基因和雪花莲凝集素抗虫基因构建于同一表达载体上进行遗传转化，获得带有安全标记基因的抗褐飞虱转基因植株。

This papersummarizes the studies on glycinin including components,structure and gene families.The homogeneity of the glycinin gene sequences was compared by bioinformatics.Heredity Distances of the six glycinin genes were analyzed. Special emphasis is laidupon comparing the sequences and components of amino acids in the glycinin. Thecontents of major amino acids in the intact glycinin and in the acid and basic peptideswere analyzed. In view of others and our researches, we advance some new thinkingand tactics of improving rice nutritional quality by using glycinin genes.

本文在简要介绍大豆球蛋白组分、结构及基因家族后，采用生物信息学方法分析比较了不同种类大豆球蛋白基因之间的序列同源性，并对不同大豆球蛋白基因的遗传距离作了分析；本文还重点比较分析了不同大豆球蛋白的氨基酸序列和组成，以及不同酸性肽和碱性肽中重要氨基酸的含量，基于对前人工作的总结和我们的分析结果，本文提出了利用大豆球蛋白基因进行水稻营养品质改良研究的新思路和新策略。

Results BLASTn analysis revealed insertion or substitution of 18-38 nucleotides in the penA gene of gonococcal isolates with reduced ceftriaxone susceptibility.

将11株头孢曲松低敏和2株头孢曲松敏感的淋球菌penA基因全基因测序，通过BLASTn与BLASTx分析，研究penA基因的碱基插入和置换情况及PBP2中氨基酸插入和置换模式。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。