基因的

In this paper, three gene S-adenosylmethionine decarboxylase, ornithine aminotransferase and betaine aldehyde dehydrogenase plus one house-keeping gene had been cloned and their expression levels under different stresses were evaluated by real-time PCR technique.

已克隆S-腺苷蛋氨酸脱羧酶、鸟氨酸氨基转移酶、甜菜碱脱氢酶3个基因和内参基因的基础上，应用定量PCR技术，对这3个基因在不同胁迫时间下的表达水平进行分析。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer, In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed.

加入诱导剂RU486后，可以诱导表达荧光素酶，并在一定范围内两者呈正比，最高可以实现荧光素酶的40余倍的表达，而没有RU486时，几乎没有报告基因的表达，表明RU486诱导调控载体构建成功，可实现对目的基因的表达时间和表达水平的精确调控，为进一步的基因调控研究和基因治疗提供了良好的工具。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer. In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed. There was a positive correlation between the luciferase activation and RU486 concentration in a definite range. The results showed that the RU486-inducible regulatory vector was successfully constructed, which can be used to regulate the expression level of genes of interest in appropriate time by the inducer RU486. This inducible expression vector provides a platform for the research of gene regulation and gene therapy.

加入诱导剂RU486后，可以诱导表达荧光素酶，并在一定范围内两者呈正比，最高可以实现荧光素酶的40余倍的表达，而没有RU486时，几乎没有报告基因的表达，表明RU486诱导调控载体构建成功，可实现对目的基因的表达时间和表达水平的精确调控，为进一步的基因调控研究和和基因治疗提供了良好的工具。

LFY gene was one of the central flower meristem identity genes in Arabidopsis.

LFY基因是控制拟南芥花分生组织特异性的关键基因，该基因的突变会引起延迟开花和叶片形态变化。

The transient expression experiment with gus gene showed that mwcsllO promoter was induced by high salt and low temperature and promoted expression of structural genes in both monocotyledonous and dicotyledonous plants.

对gus基因瞬时表达实验表明，在单子叶和双子叶植物中，mwcS12O启动子均受低温和高盐逆境诱导，启动结构基因的表达，显示其在作物抗逆基因工程改良中的应用前景。

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体，以皮肤特异人K14（人角蛋白14）基因启动子替换原质粒中的CMV启动子后，在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列，构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术，将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中，然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中，共移植了256枚受精卵，6只受体怀孕，最终获得44只成年小鼠。

The cloning, sequencing, homological blasting and Northern blotting results of 5 desiccation-induced cDNAs and 3 phosphate induced cDNAs implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as G protein, protein kinase, VP3and MAD3-like genes might be involved in dehydration in the resurrection plant Boea hygrometrica.

对其中5个脱水特异诱导表达的cDNA（包括可能与复苏能力有关的cDNA）和3个磷酸盐处理诱导表达的cDNA进行克隆测序、同源性探测和Northern杂交检测表明，牛耳草脱水过程中诱导表达的基因可能涉及到脱水胁迫的信号转导、调节基因的级联作用（VP3，MAD3样基因等）、结构基因产物调节细胞结构在脱水胁迫中的稳定性等。

Like run-on sentences, long RNAs from multiple genes are confusing and hard or impossible for the cell to use.

携带过多基因的RNA将许多临近的基因和通读基因混合到一起，根本不可能为细胞所用。

This study was designed to clone the duck FABP gene based on the sequences of chicken and mammal FABP gene by comparative genome and bioinformatics approaches; to investigate the expression characterization of FABP genes by RT-PCR; to detect the polymorphisms of FABP genes by DNA sequencing, PCR-SSCP methods, and to investigate the effects of FABP genes polymorphisms on growth and body composition traits in Kunshan sheldrake, Cherry Valley Meat duck and so on.

本研究以鸡、哺乳动物FABP基因序列为基础，通过比较基因组学和生物信息学等方法克隆鸭的FABP基因；采用RT-PCR方法研究FABP基因的组织表达特性。

Then we developed an effective method to find brain disease related gene subnetwork from the entire network.

然后，基于这一复杂脑基因网络，我们发展了从网络中寻找复杂脑疾病相关基因的有效方法，从而可以快速大规模地发现复杂脑疾病的相关基因。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。