基因的

Genetic analysis were carried out to identify powdery mildew and strip rust resistance genes in the F2 of Am6-4 amphiploid and wheat variety Jinan17. Results showed that resistances to powdery mildew and stripe rust were controlled by a single dominant gene respectively. 124 SSR primers from genome D were used for marker analysis, marker Xgwm98 150(150为下标) from the chromosome 6D was found to be linked to the new powdery mildew resistance gene with a linkage distance 20.42 cM; A special DNA band was amplified by primere xgwm33 in resistant stripe rust plants, resistance gene for stripe rust was localized on chromosome 1D, and the genetic distance between resistance gene and marker is 8.0 cM.

利用Am6-4与济南17F2分离群体进行白粉病和条锈病抗性基因的遗传分析结果证明，Am6-4中的抗白粉病和抗条锈病基因均为单显性基因；以124对D基因组SSR引物进行标记分析，引物Xgwm98在抗白粉病DNA池和单株中能扩增出特异标记带，标记与抗白粉病基因间的遗传距离为20.42cM，并将抗白粉病基因定位于6D染色体；引物Xgwm33能在抗条锈病DNA池和单株中扩增出特异标记带，标记与抗条锈病基因间的遗传距离为8.0cM，并将抗条锈病基因定位于1D染色体。

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

Strains containing cry1 genes were the most abundant in our collection (66%), and 21 different cry1-type gene combinations were found. Furthermore, several novel holotype cry genes were found and the full-length sequences of 3 novel cry genes were designated as cry54Aa1, cry30Fa1, and cry30Ga1 by B.

PCR-RFLP鉴定结果表明：此地区的苏云金芽胞杆菌主要含有cry1，cry2，cry3，cry4/10，cry9，cry30和 cry40 等7种cry基因类型；含cry1基因的菌株最丰富，共有21种不同cry1型基因组合；从中发现了新型模式基因，并采用Tail-PCR技术获得了其中3个基因的全长序列，被国际苏云金芽胞杆菌杀虫晶体蛋白基因命名委员会命名为cry54Aa1、cry30Fa1和cry30Ga1。

Result The fiber color was controlled by 4 pairs of genes at least. Whether the long fiber and linter were brown was controlled by a pair of dominant genes separately, the type of its fiber color was controlled by 2 pairs of minor genes at least, showing different types such as light brown and brown approaching white. The long fiber in F2 generation had 3 phenotypes altogether of brown long fiber and brown linter, white long fiber and white linter, white long fiber and brown linter, showing there was interaction among genes and the expression of dominant gene in brown long fiber inhibited the expression of recessive gene of linter color.

结果］纤维色泽至少受4对基因控制，长纤维和短绒棕色的有无各由1对显性基因控制，其纤维色泽类型至少还受2对微效基因控制，表现出淡棕、棕近白等不同类型。F2代长纤维共有棕色长纤维棕色短绒、白色长纤维白色短绒、白色长纤维棕色短绒3种表现型，表明基因间存在互作，棕色长纤维显性基因的表达抑制短绒色泽隐性基因的表达。

4Apoptosis associated proteins: The expression of FLICE-like inhibitory protein long form and Apolipoprotein J was upregulated and BAX membrane isoform alpha, Protein kinase B and Lymphotoxin receptor downregulated in SAMP 10 group compared to SAMR1 group.

SAMP"益气调血、扶本培元"针法组与 SAM门 0对照组相比，有 56个基因的表达不同，其中18个基因表达下调，38个基因表达上调，共涉及十二类基因。

Floral organ determination is best explained by the ABCDE model postulated by genetic studies of Arabidopsis thaliana. Sepals are determined by A and E class genes; petals are determined by A, B, and E; stamens by B, C, and E; and carpels by C and E class genes. A, B, C, and E class gene lineages are known having duplicated several times during the evolution of angiosperms. One of the noted major duplication events occurred in the origin of the early angiosperms, leading to the formation of subgroups of B/C/D/E class. Another one occurred near the basal eudicots and gave rise to further subgroups in A/B/C/D/E class genes among core eudicots. The phylogenetic position of the family Buxaceae is located right where the second major duplication of ABCDE genes might have occurred, which is supported by multiple gene (nuclear 18S rDNA, chloroplast rbcL and atpB) phylogenetic analyses.

目前经由模式植物阿伯芥的研究，建花部器官决定基因的调控，即花萼由A、E 群基因共同决定，花瓣由A、B、E 群基因，雄蕊由B、C、E 群基因，而心皮由C 和E 群基因决定。A、B、C、E 四群基因在被子植物的演化过程中发生过次的复制事件，其中比较重要的一次发生在早期被子植物演化出之时，形成B/C/D/E 群基因的次系群；另一次复制事件则发生在真双子植物基群附近，形成仅於核心真双子植物的A/B/C/D/E 群基因之次系群。

When 3 mmol·L-1 crocin treated T24 cells for 48h, the difference was significant compared with the control group ( P .05). Crocin induced wide changes of the gene expression profile of T24 cells. A total of 836 genes were up-regulated or down-regulated by more than 2 times, which were involved cell cycle controlling, DNA cell apoptosis, replication factor, and so on.

基因芯片检测发现3 mmol·L-1藏花素作用T24细胞48 h引起该细胞系基因表达谱广泛地改变，其中表达差异2倍以上的基因共836个，这些差异表达基因的功能涉及多个方面，最为明显的是与细胞生长调节相关的细胞周期调节基因、细胞凋亡调控基因、DNA复制相关基因等类型。

One of these proteins is the 2,5-oligoadenylate synthetase which is activated by double-stranded RNA. Once activated,the enzyme polymerizes ATP to a series of 2,5-oligoadenylates(2-5A),and then 2-5A activates a latent endoribonuclease which degrades viral RNA.

本文利用2-5A基因和RNase L基因，通过中间载体pJIT163将2-5A基因和RNase L基因克隆到双元植物表达载体pBINplus上，分别构建了2-5A基因的植物表达载体pBIN2-5A和RNase L基因的植物表达载体pBINRL。

Expression patterns of genes differentially expressed in starch metabolism and cell cycle during endosperm development7 DAP kernals, 15 DAP endosperms and 25 DAP endosperms of B73, ae/wx and sh1 were used to study the expression patterns of 18 differentially expressed genes in sugar metabolism and 9 differentially expressed genes in cell cycle. We found that the expression patterns of Pul, SBEⅡa, UGPase, SUS3, SSⅡa in sh1 and Sh2-1, SUS3, SSⅡa in ae/wx were altered, revealing that these genes may interact with each other.

碳水化合物代谢基因和细胞周期基因随胚乳发育的变化以B73、ae/wx、sh1授粉后7天种子、15天和25天胚乳为材料，分析18个糖代谢差异基因和9个细胞周期调节相关的差异基因的表达模式，研究发现sh1突变体中Pul、SBEⅡa、UGPase、SUS3和SSⅡa基因表达模式发生改变，ae/wx突变体中Sh2-1、SUS3和SSⅡa基因表达模式发生改变，揭示了突变体对这些基因的巨大影响，反映了它们可能存在相互作用。

In this study, we have cloned and identified two full-length cDNAs of OsBIMK2 and OsBISERK1, which encoding MAPK and SERK, respectively, and associated with benzothiadiazole induced resistance.

本文克隆和鉴定了水稻中与苯并噻二唑(benzothiadiazole，BTH)诱导抗病性相关的编码MAPK的OsBIMK2基因和编码SERK的OsBISERK1基因全长cDNA；研究了OsBIMK2基因和OsBISERK1基因在水稻—稻瘟病菌亲和与非亲和互作过程中的表达模式；原核表达了OsBIMK2基因，获得重组OsBIMK2蛋白，并研究了重组OsBIMK2蛋白的生化特性；克隆了OsBIMK2基因的启动子序列，并运用农杆菌介导的瞬间表达系统分析鉴定了启动子中参与基因表达调控的可能顺式元件；将水稻OsBIMK2基因导入烟草，获得过量表达OsBIMK2基因的转基因植株，这些转基因植株中防卫反应基因PR-1组成型表达，并提高了抗病性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。