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Projects included: Cloning and functional studies of diabetes related genes;Structural studies of thermostable Catechol 2,3-Dioxygenase by anomalous scattering; Prevention and cure, and influence factor studies of major child bearing blemish of national minority in Guizhou province.

该课题组的主要研究方向包括大规模高通量人类基因克隆及功能研究;基因芯片技术、生物信息学及功能基因组学研究;蛋白质晶体结构与功能;蛋白质热稳定机制;疾病相关基因的研究。

The muta2 tion gene of shedding hair is a single recessive gene on autosome , and the genetype were AABBccDD、AaBbccDD in inbred strain and closed colony.

确定突变基因的类型为纯合单一隐性遗传,近交系毛色基因可能为AABBccDD ,封闭群的毛色基因可能为AaBbccDD。

The article focuses on the aberrant expression and significances of proto-oncogenes, tumor suppressor genes and genes associated with the proliferation process of gastric cells such as telomerase, transcription factor and cyclooxygenase, etc. These genes, alone or synergically, participate in gastric cancer cell proliferation, resulting in an uncontrolled growth characterized by over-proliferation of decreased apoptosis of gastric cancer cells.

本文着重阐述了与胃癌细胞增殖过程密切相关的端粒酶、核转录因子、环氧化酶等基因以及参与细胞周期调控的原癌基因、抑癌基因的异常表达及临床意义,这些基因或单独作用,或相互协同,参与胃癌细胞的增殖,使肿瘤细胞生长以增殖过多、凋亡过少的失控性生长为特征。

Therefore,a sequence specific artificial zinc finger proteinis created when replacing the amino acids in the specific sites of C2H2 zinc finger protein and then fusing the protein to other activation or repression domains,while remaining the backbone of the zinc finger protein unchange...

ZFP可以介导靶基因的转录调控,抑制或激活特定基因的表达与配体依赖的靶基因激活或抑制;对DNA进行修饰,如人造限制性内切酶,重组酶,整合酶;抗病毒感染等。因此,人造锌指蛋白应用前景广阔,研究价值显著,是未来人类基因治疗的革命性的工具。

This paper studies major genes of body weight, height at withers, body length, heart girth, milk crop, and milk fat percentage on yak. The results show body weight, height at withers, milk crop, milk fat percentage have effects and roles of major genes.

但近年来的理论研究结果表明,在许多数量性状的遗传变异中,既有众多微效基因的作用,也有主基因的作用,即这些性状应属于微效多基因和主基因共同控制的混合性状[1] 。

Expression analysis with RT-PCR revealed that PcLGBP gene was exclusively expressed in hepatopancreas and the expression could be up-regulated by heat-killed Listonella anguillarum. The binding specificity of PcLGBP to ligand were subsequently examined by recombinant expression of the cDNA fragment encoding its mature peptide in Escherichia coli BL21 (DE3) and ELISA approach.

为分析PcLGBP基因的功能,通过半定量RT-PCR方法研究了其在组织中的分布,并用荧光定量PCR技术分析了其对细菌刺激的响应,结果表明PcLGBP基因是肝胰腺特异表达的基因,能响应热致死鳗弧菌刺激而增强表达,说明PcLGBP基因可能参与螯虾的细菌防御反应。

Comparison of the coding regions of BH1-BH4 genes showed that BH1 had a lower sequence identity to other previously published B-hordeins than the other three B-hordeins obtained in this study. BH1 was then cloned in a bacterial expression vector based on bacteriophage T7 RNA polymerase. The resulting plasmid produced a 28.15 kDa protein in Escherichia coli. The potential value of B-hordein genes in grain quality improvement of hull-less barley has been discussed.

并且,在4个基因BH1~BH4中,BH1与先前报道的B组醇溶蛋白基因有较低的序列相似性,因此我们对BH1基因进行了原核表达,含该基因的表达载体在大肠杆菌中表达出相对分子质量为28.15 kDa并以包涵体形式存在的蛋白,进一步对其在青稞谷粒品质改良中的潜在价值进行了探讨。

The functional allele at the W locus is Ipmyb2, which was newly found in Chinese populations, and it differs mainly in the intron regions from those of the reported allele Ipmyb1; in comparison to Ipmyb2, the newly found second allele ipmyb2 has eight substitutions in the exons, a 6-bp deletion, and a 19-bp deletion, causing ORF shifting, and exists mostly in white-flowered individuals.

以上结果表明:1花青素代谢途径上酶基因的位点均有一定程度的多态,其中尤以ANS为最高;调控基因ipmyb2的核苷酸缺失造成该基因停止功能,使其纯合子的个体开白色花。2授粉者所介导的花色表型选择确实在中国种群中发生,有可能进一步增加花色代谢途径上基因位点的遗传变异。

These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages. Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars.

马庆虎研究组分离了在小麦茎秆中高度表达的木质素合成关键酶基因-咖啡酸甲基转移酶基因,通过生化和转基因分析证明COMT 在控制松柏醇型木质素上发挥着重要作用,COMT基因的表达在抗倒伏小麦生长发育后期明显高于易倒伏品种,这种基因的高表达进一步促进了COMT酶蛋白和酶活力的提高,并增强了木质素的合成。

SiRNAs were tested for silencing expression of the major capsid protein encoded by tiger frog irus, an iridoirus causing seere disease in fish. siRNAs targeting mcp gene effectiely inhibited TF replication in fish cells as demonstrated by reduced mcp RNA leel, postponed emergence of cytopathogenic effect, as well as reduced TF titer and particles in cells.

TF的MCP基因编码一个主要衣壳蛋白,通过转染针对MCP基因的siRNAs能减少了MCP基因RNA水平,从而沉默MCP基因表达;在FHM细胞上延迟了CPE的出现,降低了TF的滴度和病毒粒子的数目。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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