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Based on the understanding of physiological pathway for broodiness, three genes related to broodiness including PRL, PRL receptor, and pituitary-specific transcription factor (POU1F1)-encoding genes were selected as candidates to screen the genomic variation by means of PCR-SSCP protocol and analyze the association with chicken broodiness and other target traits.

禽类血浆PRL水平上升与就巢行为的起始和维持具有密切的相关性,本研究采用候选基因的策略,通过分析就巢行为发生的生理机制,选择与就巢性状密切关联的PRL、PRL受体和PRL转录调控因子——垂体特异转录因子(pituitary-specific transcription factor,POUlF1)基因,采用PCR-SSCP分析检测基因变异,并分析基因变异与就巢性和其它目标性状的相关性。

Sequence structure of a cloned gene is an important factor to determine its expression level in E.coli. In this paper, we studied the expression of a fragement of HIV-1 gp120 gene and a fragement of choler toxin A subunit gene in E.coli using a new expression strategyoptimizing the gene sequence as a whole.

外源基因的序列结构是影响外源基因在大肠杆菌中表达的重要因素,我们采用基于"外源基因全序列结构优化"的新的表达策略,研究了HIV-1 gp120-801和霍乱毒素A亚单位(CTA-246)基因在大肠杆菌中的表达情况。

It was showed that wheat gliadins were codominant and the female parent prevailed in F1; there were 9 bands segarated as a pair of gene, for example, Gli-23.2, 34.8, 40.2, 46.2, 71.5, 73.8, 13.9, 26.0, 50.0 and there were 9 bands separated as two pairs of genes in F2, in addition, Gli-17. 8 did not follow with any one of them.

结果表明,醇溶蛋白在F1代表现出共显性和倾母性遗传特点;F2代蛋白谱带发生分离,具体表现为:Gli-13.9、23.2、26.0、34.8、40.2、46.2、50.0、71.5、73.8等9个组分的分离符合一对基因的分离规律,而Gli-12.7、15.7、16.5、19.1、26.9、30.5、30.8、32.2、76.8等9个组分的分离符合两对基因的分离规律;Gli-17.8既不符合一对基因的分离规律,又不符合两对基因的分离规律。

Among the 39 SUT genes analyzed in plants, the SUT genes of rubber tree shared the highest homology in amino acid with the SUT genes of Manihot esculenta and Ricinus communis, which all belong to the same family of Euphorbiaceae.

在所分析的39种植物SUT基因中,HbSUT基因与同为大戟科的木薯、蓖麻和乳浆大戟SUT基因的同源性最高,反映这些基因的系统进化与物种进化的一致性。

Microparticle can carry antisense MCP-1 gene into aorta successfully, which can inhibitate MCP-1 gene expression, microphage infusion and slow down the development of AAA. At the same time, there is no MCP-1 gene expression in other position, suggesting the microparticle si an secure gene transfer carrier.

以微粒子为载体,能成功地将反义MCP-1基因导入动脉组织中,并能明显抑制内源性MCP-1基因的表达和巨噬细胞在动脉壁的浸润程度,减缓腹主动脉瘤的进一步生长,同时外源基因在机体的其他部位没有表达,提示微粒子是一种安全的基因转移载体。

The central message of Midge Hill's article on out-crossing is to enter into the process deliberately with set goals.

当决定做异种杂交时,我们必须谨慎的设定做鱼目标,虽然此法有重新引进新基因或找回失去的基因的功效,但是同时也具有引导孔雀鱼的基因回复到野生鱼种时的基因表现。

We demonstrate that both the IL-5 mRNA expression and IL-5 protein production were stimulated by TSA and NaBu treatments. Chromatin immunoprecipitation assays showed that treatments of TSA and NaBu caused hyperacetylation of histones H3 and H4 at the IL-5 gene promoter in Jurkat cells, which consequently promoted the exogenous luciferase activity driven by this promoter.

TSA和丁酸钠能促进IL-5基因的mRNA和蛋白的表达水平,并增强该基因启动子所介导的外源荧光素酶报告基因的活性,这一增强作用与两种抑制剂介导的IL-5基因启动子上组蛋白H3和H4的高乙酰化有关。

But it is not widely used in parasitology.

目前,该技术在寄生虫学研究中的应用不是很多,主要应用在寻找与寄生虫不同发育阶段相关的差异表达基因和与寄生虫抗药性相关的差异表达基因以及其他相关基因的研究,如与不同虫株、不同宿主、或性别差异相关的基因。

The major gene heritability and polygene heritability were 64.17% and 14.76% respectively, which showed major genes played major role in seed setting rate.

在主基因作用的效应中,加性效应和显性效应各占主基因效应的50%;主基因的遗传率较高,分别为56.97%和44.26%;多基因的遗传率很低,分别为1.24%和3.05%;环境对结实率的影响显著,其方差占总表型方差的43.07%和52.99%。

Using TaKaRa LA PCR? in vitro Clonging Kit, a 2.3kb DNA fragment from the mutant was amplified and identified. Sequence analysis indicated that the fragment was composed of a complete proE gene and a partial proA gene.

根据枯草芽孢杆菌93151野生菌株proB基因的序列设计并合成引物,利用TaKaRa LA PCR~ in vitro Clonging Kit试剂盒,克隆突变菌株的proBA基因,得到一个约2.3kb的DNA片段,序列分析表明该片段包含一个完整的proB基因和不完整的proA基因。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。