基因的

Haemolyticus had a closer relationship with ACME-arcA rather than with native arcA in Staphylococcus aureus and Staphylococcus epidermidis. A novel ccr allotype ccrAB_(SHP was identified in ACME-arcA-positive isolates.

ACME-arcA基因、arcA固有基因系统进化分析显示，MRSH的arcA固有基因和金黄色葡萄球菌、表皮葡萄球菌、溶血葡萄球菌的ACME-arcA基因的亲缘关系更近。

The results showed the resistance was controlled by at least two pairs of genes, and was incompletely recessive. Effect of backcross was very significant and that of reciprocal cross was not. Resistance was dominated by nuclear genes, narrow inheritability was comparatively high. The resistance was in keeping with additive-dominance model, and dominance was dominating, and showed a quantitative character.

结果表明：苦瓜对白粉病的抗性受两对以上基因控制，两对主基因抗病相对感病为不完全隐性，回交效应极显著，正反交效应差异不显著，抗病性主要受核基因控制，两对主基因的狭义遗传力较高，符合加性－显性模型，加性效应较大，抗性效应在亲本和F1间存在极显著正相关，表现为数量性状遗传的特点。

In this exper iment,we took the advantage of RNAi technique,microinjected tinman and singless dsRNA into the early embryos in Drosophila respectively and got these two genes' RNAi phenotypes,which were very similar to that of their mutant,showing heart tube defects or no heart precursors formation.tinman dsRNA eve caused visceral mesoderm defects and the somatic muscles disruption,yet wingless dsRNA only affected heart precursors and had no effect on visceral mesoderm and somatic muscles,indicationg that the heart-related genes dsRNA interference worked effectively and exclusively in Drosophila.

实验运用RNAi技术，分别将tinman和wingless的dsRNA注入果蝇的早期胚胎，得到了这两个基因的dsRNA干扰表型，与两个基因的突变体表型非常相似，都表现为果蝇心脏前体细胞不能形成或心脏管缺失。尤其是tinman基因的dsRNA，还引起了肠中胚层缺失和体壁肌肉组织的紊乱，而wingless基因的dsRNA却只影响心脏的形成，而不影响肠中胚层，说明dsRNA干扰具有非常强的特异性，因而不失为研究果蝇心脏发育基因功能的有效方法。

The cDNA document of ACC oxidase gene of Citrus aurantium L. was cloned. By using the program of BLAST on NCBI GenBank database, the sequence presented a very high match with the ACC oxidase genes from other plants. The base sequence was analysed by using biology programe of DNAStar 5.0. 278 amino acids were coded by the base sequence and the base sequence had the same conserve region of ACC oxidase gene of many kinds of other plants. The base sequences comparability was more than 72% compared with those of many kinds of other plants. The amino acid sequences comparability was more than 70% compared with those of a lot of other plants.

克隆了酸橙1-氨基环丙烷－1-羟酸氧化酶基因cDNA片段，将片段序列在NCBI网站上进行同源性搜索，显示的皆为不同植物的ACC氧化酶基因，因而认为所克隆的片段就是酸橙ACC氧化酶基因；并运用DNAStar5.0软件进行序列分析，推导的氨基酸序列为278个残基；具有所有植物ACC氧化酶基因共有的保守区域；与多种植物的ACC氧化酶基因的核苷酸和氨基酸序列的同源性都在72%和70%以上。

The response begins with the recognition of plant resistance with the corresponding avirulence gene from the pathogen.

这种反应由植物抗病基因与病原菌无毒基因的相互识别开始，由R基因下游的一些基因整合不同的抗病信号，通过水杨酸将抗病信号传递下去。

And genetic analysis and mapping of avirulence genes from the cross between M. grisea isolates were also studied for aiding to the positional cloning and further functional studies of avirulence genes.A total of 482 isolates were collected from 13 areas in China. Each isolate was subjected to DNA fingerprint analysis using pot2-rep-PCR.

同时，通过获得与稻瘟病菌无毒基因紧密连锁的分子标记，不仅可利用该无毒基因的标记作探针，研究该无毒基因在自然群体中的分布情况，揭示病菌群体毒性组成和变异特点，且为进一步物理作图法克隆该无毒基因奠定基础。

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后，检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况，结果如下：BA诱导的基因删除可发生于NIH3T3转化细胞系，但不能发生于HeLa转化细胞系；位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的；外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

In the NPC cell lines,loss of BLU expression correlated with hypermethylation of the 20 CpG sites in the 5′ region of BLU(approximately -292bp to -23bp upstream the translation start site)as revealed by bisulphite sequencing. Expression of the gene was restored aftertreatment with 5-aza-2-deoxycytidine in CNE2, suggesting that repression of BLUtranscription might at least be partially mediated by promoter methylation.

结果显示，BLU基因翻译起点ATG上游-292--23bp区间内的20个CpG位点在5例细胞系中均被完全或者部分甲基化，与基因的表达缺失呈负相关关系；去甲基化试剂5-Aza-CdR处理CNE2后基因表达部分恢复，说明甲基化至少是引起BLU基因转录抑制的部分原因。

Green fluorescent protein gene was conjugated to the 3 end of the PAP gene in order to screen easily of the transgenic cotton plants. The combined gene was cloned into plant expression vector pBI121 and then transformed. About 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays. This result reflected that the GFP gene was expressed in the transgenic cotton; dot-blot result also demonstrated that PAP gene was exactly transformed into the cotton. The capacity of the Verticillium dahliae-resistance is being analyzed.

为了便于转基因棉花后代的筛选，在 PAP基因的 3'端融入了绿色荧光蛋白GFP）基因，然后将融合基因克隆在植物表达载体PBI 121上，再进行遗传转化，得转基因棉花种子5000余粒，将种子播种长到于叶展开时，先在黑暗中用紫外灯照射，查找表现绿色荧光的幼苗，然后再用地高辛标记的PAP基因特异性探针对这些棉花进行点杂交，最后发现有8株棉花表现阳性反应，说明PAP基因的确己经转到了棉花的基因组中，其棉花黄萎病的抗性鉴定正在进行之中。

Xanthus DK1622 by PCR, and inserted the fragment into a site upstream of lacZ, resulting in the recombinant plasmid pZCY13. The plasmid pZCY13 was transformed by electroporation to DK1622, producing a mutant ZC16-18 ?

从黄色粘球菌DK1622基因组上PCR扩增MXAN1334基因内部部分片段，插入载体pZCY11上lacZ基因的上游，构建重组质粒pZCY13，将其转入DK622菌株，获得MXAN1334基因插入失活突变株ZC16-18。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。