基因的

Porcine uncoupling protein-3 gene was used to study its effect on carcass and mest quality traits.

以猪解耦联蛋白基因3（ UCP3）作为控制猪胴体与肉质性状主基因的候选基因。

Porcine uncoupling protein-3 gene was used to study its effect on carcass and meat quality traits.

以猪解耦联蛋白基因3（UCP3）作为控制猪胴体与肉质性状主基因的候选基因。

We put emphosis on the establishment of standard protocol,testing strategy and methods in the study,in addition to the analysis of the positive ratio of GJB2,SLC26A4,mtDNA 1494/1555 in groups of different phenotypes, analysis of genotype,phenotype and its relationship of GJB2 and SLC26A4,drawing the audiograms of GJB2 and SLC26A4 related hearing impairment.Finally,some practical problems that we may come into in genetic testing are discussed.

其中，着重论述临床耳聋基因诊断的规范化流程，以及检测内容、策略和方法，分析GJB2、SLC26A4、线粒体DNA 1494/1555突变在不同受检者群体中的阳性检出率情况，分析GJB2基因和大前庭水管综合征相关SLC26A4基因的基因型和表型以及它们之间的关系，绘制GJB2相关耳聋和EVAS的听力曲线模型，并讨论在临床耳聋基因诊断过程中需要注意的一些具体问题。

Identification of susceptibility genes for complex diseases in animal model, here rat arthritis as an example, may include the following steps:①selecting inbred animal strains and establishing disease models;② segregating breeding in F2 animals and running linkage analysis to find QTLs;③ producing a series of congenic strains to narrow down QTL region under help of STR or SNPs markers;④ carrying out positional cloning of susceptibility genes, and ⑤ confirming the function of susceptibility genes.

以大鼠类风湿关节炎模型为例，定位克隆易感基因步骤包括：①选择亲代近交系，建立关节炎模型；②分离育种，连锁分析、确定数量性状位点；③建立Congenic系、窄化QTL区域；④位置克隆基因；⑤易感基因的功能验证。

Results The two patients with primary dystonia were found to have the gene mutation of SCA3, the CAG repeat were 80 and 75 respectively.

结果 2例原发性肌张力障碍患者SCA3基因突变，其异常等位基因的CAG重复数为80和75；SCA1、SCA2基因无突变。

Moreover, MeJA induced defense genes, such as pathogenesis-related proteins (PR2a), phenylalanine ammonia-lyase, BX9, and TPS. MeJA also increased DIMBOA and Vannillic acid content of leaves and roots significantly but leaf p－Hydroxybenzoic acid concentration was decreased significantly and Protocatechuric acid was produced in roots only.

1MeJA叶片处理能诱导叶片和根系中丙二烯氧化物环化酶基因、糖基转移酶（BX9）基因、苯丙氨酸转氨酶基因的表达，使叶片和根系中丁布含量升高，同时，6μL/L MeJA处理使第二叶中原儿茶酸含量显著降低，诱导根中产生原儿茶酸，使第二叶中香草酸含量和根中未知物质峰面积显著增大。

Using plant bivalent expression vector pCAMBIA1300 and pBI121 as basic components, a new binary vector harboring tomato chitinase gene Chi3 and tomato β-1,3-glucanase gene Glu-Ac, was constructed through sequential restriction digests and ligations recombination. The two genes driven by CaMV35s promoter were successfully transferred into watermelon cultivars "ZhongYuYiHao" via Agrobacterium-mediated transformation.

构建了同时含有番茄儿丁质酶基因（Chi3）和β-1，3-葡聚糖酶基因的双价抗真菌基因植物表达载体，以西瓜子叶块为外植体，采用根癌农杆菌介导法，将Chi3和Glu-Ac同时导入西瓜栽培种&中育一号&，共获得46株抗性再生植株。

For further confirming the new genetic model, a set of NILs carrying different number of the resistance genes were developed, genotypic analysis of NILs clearly show that two dominant complementary genes cooperatively control the resistance to maize dwarf mosaic in maize.

两个显性互补抗病基因的发现和定位，丰富了我国玉米矮花叶病抗病基因资源，为标记辅助选择和抗病基因克隆以及抗病育种工作提供基础。

The PTZ plasmid vector was changed to plasmid vector pSP72, which contains both T7 and Sp6 promotors for transcription of antisense (T7) and sense (Sp6) RNA probe in vitro. The results revealed the label rate of cRNA probe is high, the hybrids between RNA-RNA are stable, so the background of hybridization is satisfactory.

本实验将质粒载体PTZ转换为带有T7和Sp6两个启动基因的pSP72载体，故可在体外转录cRNA时同时得到意义（Sp6为启动基因）和反意义（T7为启动基因）探针，实验结果表明，cRNA探针标记率高，杂合物稳定，故所获杂交反应背景较为满意。

The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代，取第5、10、15和20代重组病毒进行以下检测：用蓝斑试验鉴定各代重组病毒纯度；用PCR方法扩增猪瘟病毒E0、E2基因，进行基因序列分析；用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。