基因的

In population genetics, gene flow is the transfer of alleles of genes from one population to another.

在群体遗传学中，基因流动是基因的等位基因从一个群体转移到另一个群体。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

Results The activity of FⅫ were 52.5%, 78.6% and 89% for the proband, his father and mother, respectively. Direct sequencing suggested that the proband was 46 T/T genotype in exon1, while both his parents were 46 C/T genotype in the same alle.

结果 先证者及其父、母FⅫ：C分别是52.5%、78.6%、89%，直接测序结果提示先证者F12基因的第1外显子46位碱基即启动子上游-4位为T/T基因型，其父、母在该位点均为C/T基因型。

In S. erythraea, pZW could express reporter genes thiostrepton resistance gene and enhanced green fluorescin gene. And it is more suitable to express exogenous genes in S. erythraea stably.

在糖多孢红霉菌中，pZW可以表达硫链丝菌肽抗性基因和绿色荧光蛋白基因，是更适合在糖多孢红霉菌中稳定表达外源基因的整合型表达载体。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya，以绿色荧光蛋白基因为报告基因，构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46，荧光显微镜检测发现，此启动子在两菌株中都具有功能。

Fine mapping of Helminthosporium turcicum resistance gene Ht2 is extremely valuable for map-based cloning of the Ht2 gene,gaining a better knowledge of the distribution of resistance genes in maize genome and marker-assisted selection in maize breeding.

精细定位玉米大斑病抗性基因Ht2对于图位克隆该基因和探讨玉米基因组中抗病基因的分布规律及分子标记辅助选择有重要意义。

Objective: Fibrinolytic enzyme gene of Gloydius intermedius was cloned,then analysis the gene sequence and function.

目的：克隆中介蝮蛇纤维溶解酶（Fibrinolytic enzyme，FLE）基因，分析其基因序列并对该基因的功能进行分析。

Fibrinolytic enzyme gene of Gloydius intermedius was cloned, then analysis the gene sequence and function.

克隆中介蝮蛇纤维溶解酶(Fibrinolytic enzyme， FLE)基因，分析其基因序列并对该基因的功能进行分析。

GAP gene was found to be a good internal control for the first time for studying gene expression in H. polymorpha. It was shown by Northern hybridization that H-OLE1 gene was expressed in H.

为研究H-OLE1基因的转录及其调节规律，通过一系列实验，首次找到了可在研究多形汉逊氏酵母基因表达时用作内标的GAP基因。

A highly conserved sequence of 210 bp of AtNHX1 gene that encodes a Na+/H+ antiport protein was isolated from A.thaliana,the 210 bp fragment was amplified as RNAi target site and used in construction of RNAi vector,and was inserted into pHANNIBAL as reverted repeat with an intron.The expressional box was linked into the vector of pART27,NPTⅡgene is the screening marker in this vector.

利用RNAi研究植物基因功能，可选择靶基因的一段单一序列使基因家族中的单个成员沉默；也可以选择某一基因家族的多个成员具有的一段同源性很高的保守序列，设计针对这一序列的dsRNA表达载体使多个基因同时沉默，RNAi成为功能基因组学研究的重要手段。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。