基因的

Results 1. For the normal group,20.2%(101/500) specimens were detected positive forHPV infection. 1512 women were included in the abnormal group and 523(34.6%)specimens were detected positive for HPV DNA, including 407(77.8%) one-typeinfection, 82(15.7%) diplex infection and 34(6.5%) multiple infection.

收集宫颈癌组织标本，针对HPV16亚型分别设计L1、E6和E7基因特异性引物，用PCR方法从宫颈癌组织中获得各基因的全长序列，并构建克隆载体pMD18-T，进行序列分析，同时分别以L1、E6和E7基因序列进行进化分析。

We can make use of chip to analysis virus gene group; construct mutation detection technique of heredity diseases and become normal detection technique of many heredity diseases; supervise the changes of cell gene expression, research pathogenic theory of virus; differentiate bacteria gene types and identify bacteria strains, provide base data for screening and supervise drug-fast gene.

利用基因芯片可加速对病毒基因组的功能分析；可建立遗传病的突变检测技术，并成为对多种遗传病的常规诊断技术；可用来监测宿主细胞基因表达的改变，研究病毒的致病机理；基因芯片还可用来对细菌基因的分型和菌种鉴定，为筛选和监测细菌的抗药性基因提供基础数据。

Lividans. Using pIJ903 bearing tsr gene as a vector, the two furthermost fragments of the cluster, 4. 0kb and 1. 5kb in size respectively, were inserted into it. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. OriT from E coli plasmid RK2 was also incoporated into the vector, therefore, pIJ903 derivatives can be mobilized from E.

在具有硫链丝菌素抗性基因的pIJ903的单—BamHI位点，同向插入了FR-008生物合成基因簇两个最远端的4.0kb和1.5kb片段，并在二者之间插入了可在链霉菌FR-008和变铅青链霉菌中表达的阿泊拉霉素抗性基因，同时也插入了有助于将质粒引入链霉菌的oriT片段，从而构建出了置换整个基因簇的基因置换质粒pHZ691。

MAIN OUTCOME MEASURES: The comparison of the distribution of ACE alleles and genotype in both groups of patients.

主要观察指标：两组患者ACE基因的等位基因和基因型分布比较。

Inan attempt to select more efficient biocatalysts ,the hydantoinase and carbamylase genes fromBurkholderia pickettii were cloned in Escherichia coli, achieve a co-experssed strains. The geneswere assembled to give the carbamylase gene preceding the hydantoinase gene. The recombinantstrains stably and constitutively produced the two enzymes and efficiently converted thecorresponding hydantoins onto p-hydroxyphenylglycine and phenylglycine .

我们试着寻找更快更有效的转化菌株，把来源于皮氏伯克霍尔德氏菌的表达该两种酶的两个基因同时克隆到大肠杆菌中，获得共表达两种酶的基因工程菌株，两个基因的顺序是 Dcase 基因在 Dhase 基因的前面，同一个启动子。

During the last decade, many studies have hypothesized that the MADS-box proteins could form specific dimers, which would be further assembled into tetrameric complexes to have more functions. In order to prove whether BCFMADS and CCFMADS genes can interact with each together to form either homodimer or heterodimer, we used an in vitro approach to analyze protein expressions for both genes.

MADS-box基因中，基因并不会单独的表现，而是透过形成同质二元体、异质二元体或是蛋白质复合体来表现其基因的功能性，因此本研究为欲证实BCFMADS以及CCFMADS基因间可否形成同质二元体或是异质二元体，乃利用E。

The hygromycin resistance gene is a marker gene to help sort out the transgenic mushroom cells from the non-transgenic cells, Dr. Romaine explained. What we are doing is taking a gene, as for example a drug gene, that is not part of the mushroom, and camouflaging it with regulatory elements from a mushroom gene.

潮霉素基因是一个标记物，它能筛选转化了和未转化的蘑菇细胞，Romaine教授解释说，我们做的就是得到这样一个基因，例如它是一个药物基因，它不是蘑菇基因的本身的组成部分，它伪装成蘑菇基因要做一个调整。

The results of in situ hybridization showed that the gene of Mdde was expressed mainly in epidermis of the body wall and fat bodies, and no expression signal was detected in tracheae, intestine and musculature. The gene of Mdde in epidermis expressed both in challenged larva and no-challenged larva, whereas the gene of mdde in fat body expressed only in challenged larva.

原位杂交的结果表明：Mdde基因在家蝇幼虫体内主要表达于表皮和脂肪体，在气管、肠和肌肉组织中没有检测到Mdde基因的表达；表皮中Mdde基因在微生物刺激前后均有表达，而脂肪体中Mdde基因经微生物刺激后才表达，刺激前不表达。

Bioinformatics was used to seek the homologous gene of EXT1 in Strongylocentrotus purpuratus which was just discovered and had the correlation to human hereditary multiple exostoses. The sequence, exons, coding protein, physical and chemical characters of EXT1 gene were analyzed. The structure and function of its coding protein were predicted, and the phylogenetic tree for the homologous gene was constructed, which provided certain basis for the future research of human EXT1 gene.

利用生物信息学方法寻找人类遗传多发性外生性骨疣EXT1基因在紫色球海胆中的同源基因，对该基因的序列、外显子信息、编码蛋白及其理化性质进行分析，并预测其编码蛋白的结构与功能，构建其同源基因的系统进化树，旨在为进一步研究人体EXT1基因提供一定的依据。

Methods(1) Construction of plant expression vector containing RS geneThe cloning vector pT-RS was constructed containing RS gene extracted from the leaf tip of Vitis vinifera by PCR amplification and RS sequence was identified by PCR amplification,enzymes digestion and sequence analysis.The plant expression vector containing RS gene was obtained and RS sequence was identified by PCR amplification and double enzymes digestion too.

研究方法(1)含白藜芦醇合酶基因的植物表达载体的构建提取葡萄基因组总DNA，通过PCR扩增得到RS基因，将此基因连接到克隆载体pGEM-T Vector，得到重组载体pT-RS；经PCR、酶切及序列分析鉴定后，将RS基因克隆到植物表达载体pBI121，得到重组载体pBI-RS，用PCR及双酶切方法进行鉴定。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。