基因文库

This is the first time to construct the alpaca skin cDNA library and its gene expression profile, from which

首次构建了羊驼皮肤cDNA 文库和基因表达谱；获取的大量

In the international set up on the first alpaca skin cDNA library and the sequencing, more than 7000 genes were included the United States NCBI website.

在国际上建立了第一个羊驼皮肤cDNA文库并进行了测序，7000多个基因被美国NCBI网站收录。

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

One cDNA clone was identified by immunoscreening from a cDNA expression library prepared from Babesia gibsoni merozoite mRNA and its complete nucleotide sequence was 2108bp in length,including one single open reading frame and encoding a 62ku protein which had high homology with that of apical membrane antigen-1(AMA-1) of other species.

通过免疫筛选法，从构建的犬吉布森巴贝虫cDNA基因表达文库中筛选到1个cDNA克隆，此cDNA核苷酸全长2108bp，包含1个完整的开放阅读框，编码蛋白的分子质量约为62ku。

Endosperm cDNA Library and Cloning Research of Phosphoenolpyruvate Carboxylase Gene

胚乳cDNA文库的构建及磷酸烯醇式丙酮酸羧化酶基因克隆的研究

Results A 462 bp length cDNA was amplified from S. japonicum cercaria cDNA library and identified to be RIRP gene cDNA by sequence analysis.

结果 从S 。japonicum尾蚴文库中扩增出一条长为 462bp的cDNA片段，经测序证实其为RIRP基因的全长cDNA。

Subsequently, specific scFv as a probe was used to screen the cercaria cDNA library of Schistosoma japonicum to get the coding genes of SIEA26~28 ku molecules.

随后以此为探针筛选日本血吸虫尾蚴cDNA文库，以期获得SIEA26~28ku天然分子候选疫苗相关的编码基因。

According to an open reading frame of 2 019-bp, the deduced protein precursor was predicted to target chloroplast.

从热处理的番茄叶cDNA文库中分离到一个全长为2213-bp的fisH基因。

Results Eleven proteins, including chymotrypsinogen B1 precursor, carboxypeptidase A1, trypsinogen 2, chymotryptic peptide C, trypsin 1, carboxypeptidase B1, kinesin superfamily proteins 3B, trypsin 2, mitochondria protein gene, elastase 3A and colipase were found to be able to bind to HCV core protein. Conclusions Proteins related with metabolism of glucose and lipid may bind to HCV core protein.

因此我们应用酵母双杂交技术筛选人胰腺细胞 cDNA 文库中的 HCV 核心蛋白结合蛋白基因，为研究 HCV 慢性感染与胰岛素抵抗（ insulin resistance ， IR ）、代谢性疾病之间的相关性及其作用机制，以及进一步研究 HCV 影响糖、脂类代谢的分子生物学机制提供一定的思路和方向。

Up to now, analysis on salt cress physiology , biochemistry and gene expression, and the construction of different cDNA libraries and mutant libraries have made salt cress a valuable model for the study of salt tolerance.

目前，对盐芥生理生化的研究、基因表达的研究，以及各种cDNA文库、突变体库的建立，已经使盐芥成为一种非常有价值的耐盐模式系统。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。