基因文库

In order to construct a normalized cDNA silencing library, a novel procedure was adopted to reduce the abundant copy number and increase the rare copy number. Then the normalized cDNA was put into pTRV vector to construct a cDNA library and this cDNA library was screened with the technology of virus induced gene silencing.

然后把均一化之后mRNA反转录得到cDNA，再与基因沉默载体pTRV重组，最后把构建好的载体通过电转化的方法转入到GV3101农杆菌中，从而建立起破色期番茄果实均一化cDNA沉默文库。

A new expressed sequence tag (GenBank accession number: BI740300) matched completely with human MOB gene was obtained.

从胎儿和成人肾脏消减杂交cDNA文库中获得了一条新的表达序列标签(expressed sequence tag，EST)序列，该序列对应于功能未知的MOB基因。

A novel rapid alkalinization factor gene of litchi was isolated from cDNA library of litchi pericarpGenBank accession No.

从已构建的荔枝果皮cDNA文库中挑取克隆测序，获得一条荔枝快速碱化因子基因（GenBank登录号：EU024484）。

We established the technical system to obtain the EST library of plant specific chromosome,which is important to gain the ESTs and genes located on specific chromosome and provide a new tools for complicated allopolyploid genome research.

本项目获得了建立植物特定染色体EST文库的技术体系，它对分离特定染色体的EST及克隆新基因有重要参考价值，也为异源多倍体植物基因组研究提供了新的技术手段。

RESULTS: Two subducting cDNA iibraries of up-reguiation and down-regulation differentially expressed genes in HUVECs after stimulated by endotoxin were constructed successfully. It was the basis for screening new endothelial cells to activate the related genes.

结果：成功构建了人脐静脉内皮细胞内毒素刺激后上调和下调差异表达基因两个消减cDNA文库，为进一步筛选新的内皮细胞活化相关基因打下基础。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage. Four positive clones containing chicken H-FABP gene were obtained.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

To study ovarian gene expression in teleosts, we constructed an adult ovary cDNA library of tilapia, Oreochromis mossambicus, and analyzed the gene expression profile using an expressed sequence tag-based strategy.

为了研究硬骨鱼卵巢的基因表达，本文构建了成年莫三比克吴郭鱼的卵巢cDNA文库，利用表达序列标签策略分析了卵巢基因的表达。

Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针，筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库，得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase，SO)有60～90％的同源性，长度为647bp，编码140个氨基酸，具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20～40％的同源性，长度为1047bp，编码279aa，是一个不完整的开放阅读框，预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80～90％的同源性，长度为1810bp，编码493aa，具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30～70％的同源性，长度为1446bp，具有开放阅读框和BTB／POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框，与玉米Cat-3基因具有81％同源性，预测R8-1-1可能具有与Cat-3类似的功能，可为转基因小麦抗逆育种提供新的基因。

We have successfully attained the fulllength cDNA sequence of FRG4, bioinformatic analysis shows FRG4 gene has 99%homology with Homosapiens synapse associated protein 1 (SYAP1), consists of 9 exons and 8 introns and located in Xp22.2. None transmembrane domains but a BSD domain was discovered, and its encoding protein is probably a water-solubility protein.

结果表明cDNA文库基础上运用热启动PCR获得FRG4全长cDNA序列，生物信息学分析显示FRG4基因与人类的突触相关蛋白有99%同源性；定位在X染色体的短臂2区2带2亚带2次亚带；由9个外显子和8个内含子组成；无跨膜区段；有一结构域BSD；该基因编码蛋白可能为一水溶性蛋白。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。