基因文库

By the bioinformatics analyzing tools in bioinformatics webs site such as NCBI （http:///www.ncbi.nlm.nih.gov/）, ExPaSy （http://www.expasy.org/） and combining else complicated bioinformatics software package ,to identify a EF-1 full-length gene from the Taenia asiatica eDNA plasmid libratory and predict the structure and function of the protein.

方法利用生物信息网站如美国国家生物技术信息中心（NCBI，http：∥www.ncbi.nlm.nih.gov/）和瑞士生物信息学研究所的蛋白分析专家系统（ExPASY，http：∥ca.expasy.org/）中有关基因和蛋白的序列和结构信息分析的各种工具，结合其它生物信息学分析软件包，从亚洲带绦虫成虫全长cDNA质粒文库中识别延伸因子-1的全长编码基因并预测其蛋白的结构和功能。

The transcription initiation start site of a predicted honeybee MLC-2 gene (myosin regulatory light chain 2) was localized on the genome sequence of Apis mellifera with the head of drone's 5'Long-SAGE tags mapping to genome sequences in this study, and the structure of promoter was predicted.

使用西方蜜蜂雄蜂头部5'LongSAGE文库中的肌球蛋白调节性轻链基因MLC-2的标签序列，在蜜蜂全基因组序列上定位了该基因的转录起始位点，并进而预测了其启动子的结构。

A shot-gun DNA sequence strategy was employed,in which the mitochondrial genome library of Muntiacus reevesi has been constructed to obtain the complete mitochondrial genome sequence.

通过建立麂属动物小麂线粒体DNA文库、鸟枪法测序，获得了小麂线粒体基因组全序列并对其基因组成、蛋白质的编码序列、tRNA基因等结构作了详细分析，这也是国内有关哺乳动物线粒体基因组全序列的首次报道。

To study oarian gene expression in teleosts, we constructed an adult oary cDNA library of tilapia, Oreochromis mossambicus, and analyzed the gene expression profile using an expressed sequence tag-based strategy.

为了研究硬骨鱼卵巢的基因表达，本文构建了成年莫三比克吴郭鱼的卵巢cDNA文库，利用表达序列标签策略分析了卵巢基因的表达。

Eg95 antigen cDNAs were amplified by PCR from protoscolex, oncosphere and adult worm cDNA libraries of E. granulosus, respectively and were cloned into pUCm-T plasmid, and sequenced. The sequences were analyzed by DNAman and GenBank/BLAST biosoftware.

方法根据Eg95基因序列设计引物，用PCR方法分别从新疆株细粒棘球绦虫3个不同发育阶段所构建的cDNA文库中克隆获得Eg95目的基因，将其克隆至pUCm-T载体、测序并进行序列分析。

Through the research we have established in planta transformation system, mutangenesis and screening system for salt sensitive mutants, and constructed a cDNA library under salt stress and cloned a serious of salt-tolerance related genes which had been confirmed the function by overexpression in Arabidopsis or antisense expression in Thellungiella. We also compared different mapping strategy through the analysis of the genetic polymorphism between ecotypes, and establishing mapping population for physical mapping.

建立了小盐芥的转化体系与盐敏感突变体诱变与筛选体系，构建了盐胁迫下的cDNA文库并克隆了一批耐盐相关基因，并通过模式植物拟南芥转化与小盐芥的基因反义表达证实其功能，通过不同生态型小盐芥的遗传多样性分析探讨了基因组作图的策略，建立了作图群体并开始进行物理图谱构建。

For the substitution and transversion of nucleotide in the same family, CHS sequences from Poaceae, Solanaceae and Papilionoideae evolved at nearly the same rate.

我们从水稻的基因组BAC文库中克隆了2个CHS基因，即B1-P7和B2-P5.B1-P7包含有完整的CHS基因，B2-P5则只克隆了外显子2和3'端的非编码区。

To explore the gene which controls the rhizomatous development as well as other favorable genes, a cDNA library was constructed.

为了探索控制其地下茎发育的相关基因及其他优异基因，初步构建了地下茎cDNA文库。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

The cDNA inserts of these clones were sequenced and typical and untypical NLS were identified.

从文库筛选出来的基因中，选取了两个基因，分别代表已知和未知、有典型NLS和无典型NLS。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。