基因文库

Much gene information reflecting the physiologic characters and functions of the white young alpaca skin were obtained.

基因信息反映了青年期白色羊驼皮肤的生理特性和功能；该文库的构建是有效地发现新基因的理想资源。

CDNA fragments of mnh6 and mtp1 genes were cloned from M. grisea appressorium cDNA library and full length DNA fragments of these genes were cloned from genomic DNA by LD-PCR.

利用长距离PCR从稻瘟病菌附着胞cDNA文库中克隆了mnh6和mtp1基因的cDNA序列，并从基因组DNA中克隆了mnh6和mtp1基因的全长DNA序列。

At present, most genes were isolated by screening the cDNA or DNA library with theprobes homologous to sequences of dicot plants, and their functions were identified by themorphology of floral organs in transgenic plant.

目前研究手段主要是利用双子叶植物的同源序列来筛选水稻的cDNA文库，获取相关基因，然后根据转基因植物花器官的表型来验证该基因的功能。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

Acremonium. However, low frequency of integrative genetic transformation is a major obstacle to gene cloning in Cephalosporium acremonium, for example, screening and identification of genes from a DNA bank by complementation of blocked mutants, knock out or replacement of genes.

过去的一些研究表明质粒对顶头孢霉的转化率太低，限制了许多分子生物学技术的应用，如利用互补突变株从顶头孢霉的DNA文库筛选和鉴别基因、基因敲除或者基因替换。

Combining SSH and cDNA microarray to screen of up-regulated ESTs in the water stress Erianthus arundinaceus based on the analysis of up-regulated ESTs,a full-length cDNA sequence was cloned from sugarcane through Rapid Amplification of cDNA Ends method,and the gene expression character was analyzed by real time RT-PCR.

应用消减文库技术结合cDNA芯片技术筛选水分胁迫诱导的基因的EST序列，根据筛选到感兴趣的上调表达基因的EST序列，用RACE技术获得SSADH的全长cDNA序列，并通过实时荧光RT-PCR技术对该基因的表达特征进行分析。

Because homeotic gene mutation and floral organ variation is a direct relationship,therefore the mutant application is the current functional genomics study developmentdirection. We discovered one mutant ah from line 458, which was a line ofthe twin-seedling strain W2555.ln this study, we have investigated the morphogenesisprogress, genetic analysis of mutant traits and molecular tagging of related gene.

目前，由于在水稻中发现的花器官突变体很少，所以对水稻花发育的研究主要是利用双子叶植物MAS-box基因的保守序列设计引物，在水稻基因组或者cDNA文库中筛选与双子叶植物花器官发育相对应的MADS基因，因为同源异型基因的突变与花器官的变异是直接对应的关系，所以突变体的应用是当前功能基因组学的发展方向。

The gene for pyruvate kinase,which is involved in glycolysis and catalyze the biochemical reaction from phosphoenolpyruvate to pyruvate,was screened,cloned and sequenced from non photosynthetic Oomycetes,Achlya bisexualis cDNA library.

从真菌藻状菌纲Achlyabisexualis的cDNA文库中克隆了糖酵解途径的最后一种酶—丙酮酸激酶的基因，获得这个基因的完整阅读框架，并且对这个基因的全序列进行了测定。

Acterial artificial chromosome has several notable advantages, such as inserting large fragments, maintaining the stability of inserted DNA in E.coli, producing few chrisms, recovering inserted DNA from E.coli cell and easy in library construction.

AC文库的构建是基因组较大的真核生物基因组学研究的重要基础，可用于真核生物重要基因及全基因组物理作图、重要性状基因的图位克隆、基因结构及功能分析。

We used R1 cell line, primary established by Nagy in Canada, as ES cell to establish culture environment for ES cell, and successfully subculture R1 cell with LIF and gelatinized dishes and observed its growth characteristic.

同时，也初步建立包括129小鼠基因组文库筛选获得基因组DNA片段、基因组DNA结构分析、基因打靶载体构建、ES细胞体外培养、电穿孔基因转移、重组事件的药物选择和分子鉴定在内的ES细胞基因打靶技术。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。