基因文库

Sequence analysis and function of the genes were carried out.

一方面，利用已知的尼可霉素生物合成相关基因的DNA片段为探针，从圈卷产色链霉菌的cosmid文库中克隆出6个与尼可霉素生物合成相关的基因，并进行了基因的序列分析和功能研究。

Phage display of recombinant human lymphotoxin mutation librariesand receptor affinity screeningContraction of conformable large molecule mutation library is pivotal for molecular evolution study in vitro. In order to extend the range of the screening region of amino acid in LT gene, several receptor binding sites of LT were mutated randomly by overlap PCR amplification with the random nucleotide primers, the R46, S106, L130 combined site-directed random mutation library and R46~A52, S106~F110, R46~A52 + S106~F110 region random mutation libraries of rhLT were constructed successfully.

二。重组人淋巴毒素噬菌体库的构建及受体亲和筛选构建适合的大容量生物大分子变异体文库是进行体外分子进化研究的关键，为了扩大LT基因序列中被筛选的氨基酸范围，我们采用含随机核苷酸序列的引物，通过Overlap PCR的方法对LT的受体结合区域进行多点随机突变，分别构建了rhLT R46+S106+L130三点随机突变组合文库和R46～A52、S106～F110、R46～A52+S106～F110区域随机突变体库。

We constructed a cDNA library with developing tea-oil tree seed, which is close to mature.

我们用近成熟的油茶种子为材料，构建了油茶种子的cDNA文库，在此基础上构建了EST文库并对油茶脂肪酸合成的关键基因等进行了分离鉴定工作。

They were thought involved in anthocyanidin biosynthesis in light-sensitive "Tsuda" and light-insensitive "Yurugi Akamaru". Reverse subtractions, i.e., library 1 and library 3, were compared.

比较反向构建的消减文库1和3发现，在消减文库1中筛选到的基因片段涉及54条代谢途径，其中包括类黄酮生物合成等特异的代谢途径。

PGAD424 that has the activation domain of yeast'transcript activation factor GAL4 is a kind of shuttle vector that could replicate in both E. coli and yeast. One-sixth recombinant plasmids of this library could express fusion protein of GAL4-AD and proteins from Heliothis armigera.

通过在合成cDNA第一链时参入α〓PdCTP，显示出合成的cDNA为6.0Kb以下的连续区带，测得文库的滴度为1.5×10〓，可以代表亲本细胞的全部编码蛋白质的基因序列，即该文库具有代表性。

Hepatopancreas cDNA expression libraries of normal and disease-resistant shrimp Penaeus monodon were constructed by k ZAP Express vector. The titer, capacity, recombination ratio and the size of the inserted cDNAs of two libraries showed they were effective and could be used in the research of shrimp genes.

用λZAP Express载体分别构建了正常虾和抗病虾肝胰腺的cDNA表达文库，经鉴定其各项指标(包括滴度、库容量、重组率和插入片段长度等)后表明，这两个文库都是合格的，可用于对虾基因的克隆和研究。

A cDNA library with a tittering of 7+106 pfu/mL was constructed basing on the cotton boll shell (10 days post anthesis, dpa) RNA obtained above. The recombinant ratio was found to be ca. 90% by blue-white counting.

首次构建了构建了花后10天棉铃壳cDNA文库，未稀释文库滴度达到7×10~6，蓝白斑计数计算重组率为90％，为棉铃发育基因及其启动子的克隆研究打下了基础，目前国内外未见类似报道。

Results We obtained a cDNA library of ciliates Euplotes, the titer of which was -2.437×107cfu/mL, which could suffice for functional gene screen.

首次得到了可用于筛选功能基因的原生动物纤毛虫的cDNA文库，文库滴度为2.437×107 cfu/mL。

The objective of this paper was to discuss the main types and construction strategies of cDNA and its application to halophytes and salt-tolerant mechanism of halophytes were suggested.

旨在对cDNA文库的主要类型及其构建策略，以及cDNA文库在盐生植物新基因筛选中的应用进行综合论述，并对植物耐盐机制的研究提出了展望。

CONCLUSION摘要: Our successfully constructed cDNA libraries are fulllength libraries with high efficiency and can be screened by probes and antibodies to find the associated genes of the renal papillary adenocarcinoma.

结论摘要：我们构建的人肾乳头状腺癌及癌旁组织cDNA文库为高效、全长cDNA文库，可以用做探针、抗体等免疫学筛选，进一步探寻和肾乳头状腺癌相关的基因。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。