基因文库

A seed- specific expression gene (f128) was isolated from Setaria italica cDNA library.

从谷子 未成熟种子 cDNA 文库中克隆到 1 个种子特异表达的 f128 基因。

The swim-up method was adopted to purify the sperm from possible contamination of somatic cells and the spermatozoal total RNA extracted by Trizol was used for SAGE library analysis.

混合所有总RNA进行基因表达系列分析。结果：所建SAGE文库共获877个克隆，测序得到21 052个标签，出现两次以上的独特性标签有2 712种。

Total RNAs from the leaves treated 24hrs with 430mM NaCl and controlwere extracted in wild barley Hordeum brevisubulatum (Trink. Link..The difference of mRNA transcriptions was detected using suppression subtract hybridization.

本研究以野生二棱短芒大麦为试材，运用SSH的方法比较了正常培养和430mM NaCl盐溶液胁迫处理24hrs基因转录水平上的差异，克隆出差异表达的cDNA片段449个，构建了差异表达cDNA文库。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

The subducting cDNA hbrary constructed by suppressive subducting hybridization enriched and homogenized the differentially expressed genes of different degrees of abundance stimulated by endotoxin. It is important for understanding the activation mechanism of endothelial cells and their role in the process of tissue repair.

经抑制消减杂交建立的消减cDNA文库富集并均一化了内毒素刺激后不同丰度的差异表达基因，对深入理解内皮细胞活化机制及参与组织修复的过程具有重要意义。

The open reading frame of cellular nucleic acid-binding protein was cloned from Grass Carp cDNA library.

从草鱼肝肾cDNA文库中克隆到细胞核酸结合蛋白基因CNBP的完整开放阅读框序列。

Allen was a researcher at the Department of Neurobiology, The Babraham Institute, Cambridge, UK. Dr. Allen's research skills and techniques include DNA/RNA manipulation and purification/isolation, electrophoreses, PCR, cloning, all blottings, library screening, genetic mapping, design and construction of transgenes, artificial chromosome engineering, design and construction of vectors for gene targeting in ES cells, cell culture, histological techniques, mouse behavioural analysis, small animal surgery, and bioinformatic tools such as sequence databases and analysis tools.

从1996年到 2004年，Dr。 Allen 一直在英国剑桥Babraham 研究所神经生物学部门从事研究工作，包括DNA/RNA操纵、提纯/分离、电泳、PCR、克隆、印迹工作、文库筛选、遗传图绘制、转基因设计与构建、人工染色体工程、用于ES细胞基因打靶的载体设计与构建、细胞培养、切片技术、小鼠行为分析、小动物外科手术以及序列数据库和分析工具等生物信息工具的研究。

This library will provide a convenient platform for identifying the functional genes in ciliates.

该文库为进一步筛选和研究八肋游仆虫功能基因提供了便利的平台。

Extracting exonic sequences directly from genomic sequence is difficult, but ESTs have provided a convenient means of accessing them. An expressed sequence tag is part of a gene that results from sequencing a portion of a cDNA clone that was generated from an mRNA.

从mRNA来源的cDNA文库中随机取出一个克隆，从5'末端或3'末端对插入的cDNA片段进行单向测序，所获得EST为我们研究基因表达提供了方便快捷的手段。

Several of those newly identified proteins are of interest to our understanding of the molecular physiology of kelps, for example their carbon-concentrating mechanisms, cell wall biosynthesis and halogen metabolism.

简评：应用cDNA文库技术，进行基因表达差异研究，对于具有世代交替的藻类研究，有一定参考价值。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。