基因文库

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

Four obvious up-regulated ESTs of SSADH were obtained in the drought stress Erianthus arundinaceus througth cDNA microarray hybridization.

通过消减文库技术结合cDNA芯片技术筛选的EST中含有4个推测为基因SSADH的EST，其在水分胁迫处理的斑茅中均明显上调表达。

Protein moleculars interacted with Plk1 were screened with yeast two hybridation system, Plk1 cDNA was amplified from total RNA of murine embryonic stem cells by RT-PCR.

我们选择了实验室现有的小鼠胚胎干细胞cDNA文库作为基础，首先利用RT-PCR方法获得了小鼠Plk1基因。

The macronuelear DNA molecule carrying the GARP gene is 460 bp long and shows the characteristics of macronuelear chromosomes of hypotrichous ciliates.

一种富含GAA三核苷酸的GARP（glutamic acid-rich protein）基因从八肋游仆虫大核文库中筛选获得。

Methods By the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 fulllength gene from the Taenia saginata asiatica fulllength cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced protein were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuclease digestion and cloned into the prokaryotic expression vector pET30a and then expressed in E.coli BL21 with IPTG induction.

用生物信息学方法从亚洲牛带绦虫成虫全长cDNA质粒文库中识别TaCRISP的全长编码基因并预测编码蛋白质的各种结构与功能；将其编码区序列克隆到原核表达载体pET-30a上，测序鉴定重组质粒，之后进行诱导表达，表达产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定。

A GARP (glutamic acid-rich protein) gene was isolated from the macronuclear plasmid mini library of Euplotes octocarinatus .

一种富含GAA三核苷酸的 GARP (glutamic acid-rich protein)基因从八肋游仆虫大核文库中筛选获得。

Our strategy was constructing the cDNA expression library of the Oncosphere development stage by keeping away from the genome of Teania solium, and screening, cloning the immunogen genes, as well as expressing and analysis the interesting gene.

本研究避开庞大的基因组DNA，以构建六钩蚴发育阶段的cDNA表达文库为切入点，从中筛选、克隆免疫原基因，并进行表达和功能分析。

Objective To screen and identify the recombinants from the cDNA library of the adult Paragonimus west-ermani for immunodiagnosis and immunoprophylaxis.

目的从卫氏并殖吸虫成虫cDDA文库中筛选并鉴定可用于免疫诊断和免疫预防的基因克隆。

Leaves infected with the mixed races of Phytophthora infestans for 48 h.

R-gene-free叶片为材料，构建了一个富集晚疫病抗性相关基因的cDNA文库。

In attempt to elucidate the molecular mechanism of cold tolerance of wintersweet, which blooms in the deep winter,a COR413 protein gene, designated as Cpcor413pm1, was obtained by sequencing the randomly selected clones, on the basis of Chimonanthus praecox flower cDNA library construction and its ESTs analysis.

为研究蜡梅冬季开花过程的抗寒分子机理，在构建蜡梅花cDNA文库及EST分析的基础上，通过随机克隆测序，克隆了1个蜡梅COR413蛋白的cDNA基因，命名为Cpcor413pm1。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。