基因文库

Methods The BiVP gene was screened by the ESTs technology and cloned by PCR method from the cDNA library of the bumblebee, Bombus ignitus. It was reconstructed to form the baculovirus transfervector pBac1-BiVP and expressed in Sf-9 insect cell. The recombinant BiVP was purified by the fast protein liquid chromatography system.

利用ESTs从红光熊蜂cDNA文库中获得BiVP基因，以PCR技术扩增出BiVP的完整cDNA，并构建病毒表达载体PBac1-BiVP，通过昆虫杆状病毒表达系统，在Sf-9昆虫细胞中进行表达，表达产物用快速蛋白液相色谱系统进行纯化。

In this paper, the complete cDNA and deduced amino acid sequences of the ribosomal proteins S23 (AmphiS23) and L35 (AmphiL35) have been identified from amphioxus Branchiostoma belcheri tsingtauense gut cDNA library.

本文从青岛文昌鱼(Branchiostoma belcheri tsingtauense)消化道cDNA文库中得到两个cDNA克隆，鉴定后确定它们分别编码文昌鱼核糖体蛋白S23和L35的基因，命名为AmphiS23和AmphiL35。

The set of SSR loci would potentially be useful molecular markers for the germplasm identification, genetic diversity analysis, and gene mapping of Bupleurum.

从文库资料中挖掘的具有潜在标记价值的SSR位点将为柴胡种质鉴定，遗传多样性分析及基因定位提供有效分子标记。

In order to isolate specific functional genes involved in reproduction and endocrine regulation and to reveal their evolutionary mechanisms in Chinese sturgeon, a chondrostean fish with a history of 140 million years, we constructed its pituitary cDNA library from a 4 year-old male.

为了能在中华鲟中发现与生长和生殖内分泌调控相关的基因，我们构建了中华鲟垂体的SMART cDNA质粒文库。

Objective Screening ,molecular cloning , and identifying of encoding gene of fibrinolytic activity protein from cDNA library established from clamworm digestive tract epithelial cells by SMART(Switching Mechanism At 5' end of the RNA Transcript) technology .

①目的：构建沙蚕消化道上皮细胞的cDNA文库，并从中筛选、克隆和鉴定一种具有纤溶活性的蛋白编码基因序列。

objective to screen functional gene through construction of the cdna library of adult clonorchis sinensis.

目的 通过建立华支睾吸虫成虫cdna文库，筛选其功能基因，以研制华支睾吸虫的药物靶标。

Results cdc42-1ike protein gene was screened from the cdna library of adult clonorchis sinensis.

结果 从华支睾吸虫成虫cdna文库中筛选出cdc42样蛋白基因。

cdc42-1ike protein gene was screened from the cdna library of adult clonorchis sinensiswith bio-informatics method.

应用生物信息学方法从华支睾吸虫成虫cdna文库中筛选出cdc42样蛋白基因。

The new gene from Clonorchis sinensis was identified through the screening with the cDNA library of this parasite and such gene was cloned and constructed for the recombinant expression vector.

目的 通过筛选cDNA文库识别华支睾吸虫新基因；克隆并构建重组表达载体，为进一步研究其功能及其应用价值奠定基础。

The TC gene was screened from cDNA library of adult Clonorchis sinensis, cloned, expressed and purified. The purified protein of TC gene will be of importance for further research on the biological function of the gene.

通过生物信息学方法由华枝睾吸虫成虫cDNA文库中筛选出TC基因，并成功予以克隆，表达及纯化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。