基因文库

Objective To immunoscreen one protein antigen gene from a cDNA library of Cysticercus cellulosae . Methods A cDNA library of C.

目的 从猪囊尾蚴cDNA文库中筛选出编码某种蛋白抗原的基因。

A full-length cDNA of cytolysin was obtained by reverse transcriptase-polymerase chainreaction, using primers designed according to the EST sequence from the cDNA library.

玫瑰红绿海葵触手cDNA表达文库的成功构建，为研究海葵触手毒腺的活性组分和分子作用机制奠定了基础，也是分离新基因的重要资源。

By sequencing randomly-selected clones from the library and usingbioinformatics, 73 novel expressed sequence tags were obtained, in which therewere 24 full-length cDNAs, including cytolysin gene, neurotoxin genes, and greenfluorescence gene. Functional study on these novel genes is an ongoing task in ourlaboratory.

对文库克隆进行序列测定和生物信息学分析，已经获得了73个海葵新表达序列标签，有24个全长cDNA，其中包括海葵细胞毒素、海葵神经毒素、绿色荧光蛋白等具有开发应用价值的新基因。

Object To found a different cDNA library with Malasesiza furfur in hypha phase, and procure its gene fragment on differential expression.

目的：建立糠秕马拉色菌菌丝相细胞的差异表达cDNA文库，获得其差异表达的基因片段。

Found a different cDNA library with Malasesiza furfur in hypha phase by Subtractive Hybridization; And sequencing some fragment of differential expression gene. Provid foundation for the further study on complete differential gene expression.

采用消减杂交技术建立了糠秕马拉色菌的差异cDNA文库；并对部分克隆片段进行测序，为今后获得完整的差异表达基因打下基础。

And a novel β-glucosidase gene was cloned and sequenced from this library.

从这个文库中筛选到一个新的β-葡萄糖苷酶基因。

In this thesis, the integrated cDNA sequences of hira genes have been isolated from full-matured ovary SMART cDNA library of gynogenetic gibel carp and bisexual coloreded crucian carp by PCR and RACE-PCR.

本研究通过PCR和RACE的方法，分别从天然雌核发育银鲫和两性生殖彩鲫成熟卵细胞的SMART cDNA文库中克隆出hira全长基因。

Results: A different expression cDNA subtractive bank was successfully established from the human nasopharyngeal carcinoma cell line CNE2Z; Six cDNA clones were highly expressed in the lower metastatic strain CNE2ZH5; aligned to sequences of the GenBank database by BLASTN, the six cDNA fragments were found to homologize highly with known human genes.

结果：成功构建人鼻咽癌细胞克隆株差异表达cDNA消减文库，从随机测序10个克隆中获得6个在低转移鼻咽癌细胞株中高表达cDNA序列，它们都与已知的人类基因片段具有高度同源性。

We established the techniques of screening the plasmid expression library and cloning the immunogen genes.

建立了筛选质粒表达文库和克隆免疫原基因的技术方法。

Only one candidate gene is in the region, and the full-length ORF of SKC1 was obtained from the cDNA library of Nona by using PCR.

该区域内只有一个候选基因，利用PCR技术从Nona的cDNA文库中获得SKC1的全长ORF。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。