基因改造的

A 717 base pairs long artificial gene fragment of PrV gE has been synthesized by using preferred codons in the highly expressed Pichia genes. Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments.

通过人工合成基因的方法对编码gE蛋白的第29～268位氨基酸残基的核苷酸序列（717bp）进行改造，用P.pastoris高表达蛋白偏爱的密码子替换原有的核苷酸序列，氨基酸序列保持不变。

According to the changjiang River cotton district orientation 50s~60s yarn manufacture base, the research situation and foregroud of high quality cotton was analyzed in Hunan, Moreover, the suggestion was proposed on base of restrict element, the reconstruct of spinning and weaving technological is most pressing matter of the moment, The creating and introducing new and excellent idioplasm and material, The heredity quality improving project, The outstanding fibre quality gene project are the fundamental me...

根据国家将长江中下游棉区定位为50￣60支纱生产基地的区划要求，分析国内外及湖南棉区高品质棉的研究现状及发展前景，并针对限制湖南高品质棉发展的"瓶颈"因素提出了加强纺织技术改造，是当务之急，创造与引进优异新种质新材料、遗传品质改良工程，优异纤维品质的基因工程是根本途径，加速新品种选育是重中之重。

In order to further understand the relation of structure and function of Tpo, we modify the Tpo gene, using PCR technique to acquire N end domain of Tpo---Tpo〓 and dimmer of N end domain of Tpo---Tpo〓both of them are able to stimulate proliferation of megakaryocyte.

为了进一步了解Tpo结构与功能的关系，获得新型Tpo重组产品，我们对Tpo基因进行了改造，得到了具有Tpo刺激活性的Tpo N端结构域及两个N端结构域构成的双体。

In this report,selectin's molecular structure,function,expression,regulation and gene reformation were discussed.

本文就选择素的结构、功能、表达的调节及其基因的改造进行综述。

By adding a sequence encoding a small peptide,〓, to the base sequence of pYZFcd20, a new phasmid pYZcpp3 was constructed. Using the pYZcpp3 to transform E. coli. 16c9, the genetically engineered bacteria 10916# was obtained. 10916# can secret the soluble chimeric antibody fragment anti-CD20 F〓 into periplasmic.

通过对质粒pYZFcd20的碱基序列的改造，在CH1基因的末端添加了一截二聚化结构的编码序列，实现了在大肠杆菌中直接表达可溶性抗CD20嵌和抗体片段F〓的目的。

At present,the breeding should be established in the selecting breeding and obtain pure line to keep the stable inheritance of economic traits.

随着栽培经验的积累和科学技术的发展，紫菜的遗传育种已从最初的选择性育种发展到利用基因工程技术进行遗传性状的改造，并建立纯系使经济性状能够稳定遗传。

Being watched by John Witherspoon's character, the scene shown is of the mail man being chased by the genetically-engineered dog.

讲述的是杰瑞特博士想通过DNA的基因遗传改造，将狗主角麦斯变成更聪明、更灵活的护卫犬。

A modified Galanthus nivalis agglutinin gene was introduced into the genome of main cultivar 'Ningqi No.1' of Lycium bararum in vitro with explants of leaf disks mediated by Agrobacterium tumefaciens. Several factors affecting transformation efficiencies were studied based on induction rate of resistant callus. An effective system for genetic transformation of Lycium bararum was set up, and 70 kanamycinresistant transformants were obtained.

为了解决枸杞蚜虫侵害的难题，以宁夏枸杞主栽品种&宁杞1号&叶片为外植体，通过农杆菌介导的转化方法，将改造后的雪花莲凝集素GNA基因转入枸杞，以抗性愈伤组织诱导率作为转化效率的指标，对影响转化效率的因素进行了研究，初步建立了转化频率较高的转化系统。

Energy is stored inside the algal cell in the forms of lipids and carbohydrates, and can be converted into fuels such as biodiesel and ethanol. ENN screens fastest-growing、high-lipid content algae with high-throughput screen and genetic engineering technologies; ENN enhances light utilization efficiency and CO2 absorption efficiency with mass-cultivation and low-cost photobioreactor technologies; ENN industrializes micao algae bioenergy with waste water and heat reuse technologies.

采用高通量筛选和基因工程改造，获取生长速度快、油脂含量高、适合工业生产的优良藻种；采用高密度立体养殖技术和高效低成本光生物反应器技术，提高光利用效率及二氧化碳吸收效率；采用含氮、磷较高的工业废水回用技术和工业废热利用技术，提高养殖效率，降低养殖成本，实现微藻生物能源的产业化。

By locating relative genes or operons, genetic modification of industrial microbe could be performed to help further develop multiple biomass energy resource with un-glucosyl group, which is very meaningful to the transformation of energy pattern as well as sustainable development.

通过定位与此相关的特殊操纵子或编码基因，能够帮助实现工业微生物的改造，从而使多种非葡萄糖基生物质能源得到有效利用，对能源模式的转化和可持续发展有着重要意义。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。