基因改造的

The results showed that GFP gene and the synthetic dragline gene had integrated into the genomic DNA of silkworm and could be inherited.

由此，得到了GFP-蜘蛛拖牵丝融合基因转基因蚕，它能作为进一步用蛋白质工程改造蚕丝性能的中间体。

This paper will give simple review about the ways to prolong effect of recombined protein pharmaceuticals such as chemical modification, gene fusion, point mutaion and delivery systems.

本文对几种多肽药物常用的长效改造方法如化学修饰、基因融合、点突变、制剂作一简单综述。

Since the Km〓 gene of pCS4 and pCS11 was derived from the mesophile plasmid pUB110 and coded for a thermolabile protain, it conferred Km〓 phenotype on the host cell at 48℃ only. Mutant Km〓 gene was obtained by selecting for Km〓 phenotype at elevated growth temperature. In this way improved vectors pCS5 and pCS12 were obtained, which can be used at 65℃ by Km selection.

利用提高培养温度后选择卡那霉素抗性表型的方法，从编码不耐热蛋白质的卡那霉素抗性基因出发，得到了提高其耐热性的突变的卡那霉素抗性基因，从而改造了CS4和pCS11，得到了适用温度达65℃的改进的载体质粒pCS5和pCS12。

Gene engineering techniques have been widely used in construction of microorganism.

基因工程已广泛地应用于微生物的菌种改造与构建。

A plant expression vector pBACG containing the DNA sequence coding for Amaranthus caudatus agglutinin and a modified Glanthus nivalis agglutinin gene was constructed .

构建了含尾穗苋凝集素基因的cDNA序列和改造后的雪花莲凝集素基因的植物表达载体pBACG。

Since Watson and Crick have brought forward the model of DNA helix structure and explain about it's reproduce mechanism in molecule level, research work concerning DNA has been continuously lucubrated. However, whether translating inheritance information and rebuilding DNA molecule via menstruating DNA sequence, or to date the most compelling gene project, chromosome protract et. al, there all need a sort of molecule scissors cleaving DNA, DNA cleavage reagent. At present, tool enzyme of cleaving DNA is mostly DNA restriction enzymes founded in 70 years, which only cleave DNA at specific 4-8 base pair sequences that are usually palindromic, so this restricts the at very fast speed developing of gene technology at certain extent.

自从Watson和Crick提出DNA的双螺旋结构并对其复制机制在分子水平上进行解释后，使得对DNA的研究不断深入，但无论是通过测定DNA的序列来破译遗传信息、改造DNA分子，还是目前最令人关注的基因工程、染色体绘制等，都需要一种断裂DNA分子的&剪刀&——DNA断裂试剂，目前断裂DNA的工具酶主要是上个世纪70年代发现的DNA限制性内切酶，但所知的Ⅱ型限制性内切酶的DNA序列识别长度较短（约为4～8个碱基对），而且一般要求回文结构，这就给飞速发展的基因工程技术带来一定的限制。

The carboxyl-terminal fragment of approximately 15ku of zonula occludens toxin gene encoding the 264-399 amino acid residue was amplified by PCR and then cloned into the prokaryotic expression plasmids PBCX.

用PCR扩增了MBP-ZOT质粒上的ZOT基因，该基因编码264～399位氨基酸残基C末端的15ku ΔG片段，并与经改造后的质粒PBCX连接。

In recent years, it was used to investigate the mechanism of bacteriocin as an intermediate vector, to construct lactic acid bacteria genetic strains and develop oral vaccine, even in gene therapy study.

已用于研究细菌素作用机制，乳酸菌基因工程菌株的改造以及口服疫苗的开发等，应用领域十分广泛，已成为乳酸菌基因工程研究的重要工具质粒之一。

Well, there was once a great Soviet agronomist named Trofim Lysenko who had demonstrated that "environmental pressure" can effect eugenic changes (as that in the classical genetics of Gregor Mendel, 1822-1884) like the sowing of winter wheat in spring by first soaking the seeds in warm water and burying them in the snow for the winter for sowing in the spring, all to achieve a better crop and a 25% higher-yield harvest from season to season.

话前苏联有一个伟大的农学家名新科(主新科学的意思，原名 Trofim Lysenko)，发现孟德尔(Gregor Johann Mendel， 1822-1884)的古典遗传学是一翻废话，而〝环境压〞可快速达到基因的改造，如冬麦的春化，将冬麦的种籽用温水泡后，储於冰雪下过冬，春播的冬麦在秋割时即会丰收，但比春麦多收25％，而且基因即会优化，代接一代，成为种，这就是社会主义天堂的春化科学，应用於几乎整个天堂社会﹕包括教育、医学、政治宣传、军事等等。

Well, there was once a great Soviet agronomist named Trofim Lysenko who had demonstrated that "environmental pressure" can effect eugenic changes (as that in the classical genetics of Gregor Mendel, 1822-1884) like the sowing of winter wheat in spring by first soaking the seeds in warm water and burying them in the snow for the winter for sowing in the spring, all to achieve a better crop and a 25% higher-yield harvest from season to season.

话前苏联有一个伟大的农学家名新科(主学的意思，原名 Trofim Lysenko)，发现孟德尔(Gregor Johann Mendel， 1822-1884)的古典遗传学是一翻废话，而〝环境压〞可快速达到基因的改造，如冬麦的春化，将冬麦的种籽用温水泡后，储於冰雪下过冬，春播的冬麦在秋割时即会丰收，但比春麦多收 25％，而且基因即会优化，代接一代，成为种，这就是社会主义天堂的春化科学，应用於几乎整个天堂社会﹕包括教育、医学、政治宣传、军事等等。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。