基因改造的

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments. Ligation of these four fragments resulted in a cloned DNA MgE717 coding for the 29～268 amino acid region of gE. Sequencing result showed that there is a mutation at 255 amino acid, Asn instead of Asp.

分四段分别合成，基因拼接得到了改造后的基因片段MgE717，经测序，除了在gE基因+763位核苷酸处存在一碱基置换，导致第255位的氨基酸残基由Asp突变为Asn，其余的序列与PrV鄂A株gE基因的氨基酸序列相符。

PCT is a chess training software, developed by GM Gilberto Milos, based on memorization of key concepts and positions, which is essential and the most powerful way to become a strong player.

厘是一个国际象棋训练软件，开发的基因改造吉尔伯托米洛斯阿的基础上，记忆的关键概念和立场，这是必要的和最强大的方式成为一个强大的球员。

We adopted the codon usage of vaccinia virus to modified the codon usage of nef and expression them in two prokaryotic expression vector.

我们采用痘苗病毒总体密码子使用频率改造HIV的nef基因后，提高基因的AT含量，其对痘苗总体的CAI指数提高到0.9以上。

Subclones of the hybrid 741 took on a high insect-resistant identity to the larvae of leaf pests such as Clostera anachoreta Fabricius, Lymantria dispar Linnacus, Hyphantria cunea Drury ect., and the mean death rate of the grubs were all above the 85%, furthermore, the grubs' growth which survived could be restrained and the growth rate was reduced so that they couldn't tie cocoons as the usual.

项目简介：转双抗虫基因741杨是将部分改造BtCryIAc 基因与慈菇蛋白酶抑制剂基因，通过农杆菌介导法转化了优良白杨杂种741杨，并获得了一批具有高抗虫性无性系，对舞毒蛾和杨扇舟蛾，美国白蛾等鳞翅目幼虫死亡率达85%以上，并能抑制存活幼虫的发育，使其不能正常化蛹。

To develop novel live attenuated influenza vaccine, we explored the feasibility to attenuate influenza virus by codon deoptimization of NS1. According to the codon usage bias in influenza A virus, we designed and synthesized a condon-deoptimized NS gene by substituting codons of 110 amino acids in the NS1 gene of A/Puerto Rico/8/34(H1N1) with unpreferred synonymous codons.

根据A型流感病毒密码子使用偏嗜性，选取稀有密码子对A/Puerto Rico/8/34(H1N1)病毒NS1基因内部110个氨基酸区域进行密码子同义突变改造，并全基因合成NS基因，利用反向遗传操作技术拯救出含有密码子去优化NS1基因的重组病毒。

To enhance the aphid-resistant, gene GNA34 was directly mutagenized to revert 9 of XCGs and XTAs of the least favored codons to preferred codons in dicots without changing amino acid composition.

为了提高所克隆的GNA34基因在双子叶植物中的抗蚜活性，我们对GNA34基因进行了改造。

Captain Parker was sent in to Nod's main temple, in Sarajevo, the staging area of Commander Solomon's task force, to infiltrate the temple, learn and recover documents about Project Re-Genesis.

帕克上尉奉命前往NOD位于萨拉热窝的主要神殿，所罗门将军的特别行动部队第一步将到达的地方。他们将渗透神殿，学习并且夺取关于基因改造项目的所有文件。

This "EU Model" could be described with a three-leveled picture: first using the precautionary principle to push the GMO legislation, in order to obey the EU protection duty; then establishing a transnational political negotiating panel to weight different related interests, which is confirmed and legitimized by democracy; finally, the constructed GMO legal system is a hierarchy institutional framework, with horizontal regulations and central enforcements.

欧盟的GMO法规，首先采纳预防原则作为保护义务之立法推力，再以具备民主正当性之法益权衡机制为立法沟通与跨国政治协商的平台，最后建构出水平规范、中央执行的集中式制度性架构--此乃由具体基因改造科技规范所解析出的三阶层「欧盟模式」图像。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。