基因型

The aberrant phenotypes were observed in the anther of tobaccotransformed with ZM401 sense construct: the inflorescence growth and floral axiselongation are suppressed; the flower buds falling or flowers abscission earlier wereobserved. The degradation of tapetum are not observed at the binucleate pollen stagewhich corresponds to the mature stage, although the tapetal cells of untransformedtobacco anther at the same stage have already degenerated completely anddisappeared.

对转基因烟草进行形态学、组织学分析结果表明，ZM401基因以反向方式整合的转基因烟草表现一切正常，而含有ZM401正义基因的转基因烟草在表型和组织学上都出现了异常：花序生长缓慢，花序轴变短，生长过程中花逐渐脱落，花序轴褐化枯死；花药绒毡层退化延迟，药室内壁在雄配子体发育后期不能进行带状加厚，阻碍了花粉粒的释放。

Fragments from the 239th to the 944th nucleotide of the coding regions of all actin mRNAs were amplified with two actin specific primers. Fragments from the 239th nucleotide of the coding region to the end of actin mRNAs were also amplified The electrophoresis results were compared between the macropterous and brachypterous brown planthoppers.

利用两条肌动蛋白特异性引物，扩增出了肌动蛋白基因mRNA的编码区片段，另外，将肌动蛋白特异性引物作为上游引物，将与3'锚定反转录引物部分序列相同的引物作为下游引物，扩增出了从肌动蛋白基因mRNA的编码区第239号碱基到Poly尾的这段cDNA，并在翅型之间作了比较。

Study on glutenin subunits,GMP content and their relationships with(T.aestivum L.)With the analysis of high and low glutenin subunits of common wheat(Triticum aestivum L.)in China,the correlations between glutenin compositions,glutenin macropolymercontentand breadmaking quality were studied.Also a rapid and efficient detection method of geneticpolymorphism at Glu-D1y loci in wheat was established using polymerase chain reaction.

分析了100份小麦农家品种和241份育成品种的高、低分子量谷蛋白亚基的组成以及有关的品质性状，研究了高分子量谷蛋白亚基频率的变化，用单向一步SDS-PAGE的方法分离LMW-GS，并对我国小麦品种进行初步分类；在亚基水平上研究了不同的亚基或亚基组合对品质性状的影响；在聚合体水平上研究了谷蛋白大聚合体的含量与其他烘烤品质性状的相关性及其在面包体积预测中所起的作用；在基因水平上利用PCR技术研究了1D染色体上控制y-型谷蛋白亚基的Glu-Dly位点基因的多态性。

TaqIB polymorphism possibly contributed to cerebrocardiac vascular disease.The accidents of cardiovascular disease in B1B1 type was the highest in three genotypes while the accidents of cerebrovascular disease in B1B2 type was the highest.B2 allel gene has probably protective effect on cerebrocardiac artery.

TaqIB基因多态性与心脑血管事件发生可能有关，B1B1型心血管事件发生率较高，B1B2型脑血管事件发生率较高，B2B2型心脑血管事件发生率均较低，B2等位基因可能具有心脑血管保护作用。

WT, antisense-transgenic lines T2-8 and sense- transgenic lines T2-7 of tomato were used to measure the fatty acid composition of thylakoid membrane, photosynthetic and chlorophyll fluorescence parameters, chloroplastic reactive oxygen species and activities of antioxidant enzymes under salt stress.

以野生型，转反义番茄叶绿体ω-3脂肪酸去饱和酶(LeFAD7)基因株系T2-8和转正义LeFAD7基因株系T2-7为试材，测定了类囊体膜脂脂肪酸组成，盐胁迫后的光合参数，叶绿素荧光参数和叶绿体活性氧清除酶活性。

Japonicus used in this experimentation is matemal inheritance.5 Sequence analysis of mitochondrial 16S rRNA and COI gene and molecular phylogeny of four species of sea cucumber500 bp 16S rRNA and 540 bp COI fragment were obtained by PCR method from mitochondrial DNA of four species of sea cucumber, Apostichopus japonicus, Holothuria fuscogilva, Cucumaria frondosa and Mensamaria intercedens. Variable sites, nucleotide diversity, average number of nucleotide differences and average number of nucleotide substitutions per site were calculated to analyze sequence difference, and phylogenetic trees were constructed by the NJ and ME method.

结果显示在来自这5组家系的100个后代中仅检测到母本的单倍型，刺参的线粒体遗传模式表现为典型的母系遗传。5 4种海参16S rRNA和COI基因片段序列比较及系统学研究采用PCR技术对山东荣成、长岛、俄罗斯和日本的刺参，黄乳海参，北大西洋瓜参，二色桌片参的16S rRNA和COI基因片段进行了扩增和测序，分别得到了长度约为500 bp和540 bp的片段。

The inhibitor for DNA methyltransferase, 5-azacytidine (5-azaC), can demethylate the methylated DNA and restore transcription of genes. High dose homocystine can mediat DNA methylation and suppress gene expression.

应用甲基转移酶抑制剂5-氮杂胞苷(5-azacytidine， 5-azaC)可以使甲基化的启动子去甲基化，启动基因重新转录；高浓度同型半胱氨酸(homocystine， Hcy)可以介导基因甲基化而抑制其表达。

Additive effects were predominant, totally explained 75% of the phenotypic variation and often combined with digenic epistasis. Of 12 main QTLs,9 showed Gaoyou alleles decreasing plant height. Most QTLs with QE effects showed ecologically favourable alleles in diverse regions.

株高受多个QTL影响（12个位点具有加性或兼有环境互作效应，5个位点具有互作效应），以加性效应为主，加性效应总和可解释定位群体表型变异的75%左右，并多兼有上位性效应。12个主效QTL中，9个是&高油&等位基因相对&Sollux&有降低株高的作用，大多数加性×环境互作QTL的有效等位基因具有环境选择特异性。

With this modified method the genetic polymorphisms of HLA-DRB1 gene were examined in five populations, Lahu, Naxi, Pumi, Yao and Drung ethnic groups, from Yunnan Province of South China.

本文首先对HLA-DRB1基因的分型方法，PCR-SBT方法进行了改进研究，然后应用该方法对中国云南拉祜族、纳西族、瑶族、普米族和独龙族等五个民族进行了HLA-DRB1基因的多态性研究。

Objective: To explore the inhibition effects of ectogenic wild-type p53 cDNA (Ad-wtp53) on colorectal carcinoma cell lines with different p53 gene status and search for the role of wild type p53 tumor suppressor gene in occurrenc and progress of malignant tumor.

目的：探索重组腺病毒介导的外源性野生型p53cDNA(Ad-wtp53)对内源性p53基因状态不同结直肠癌细胞的抑制作用是否存在差异，研究p53基因抑制肿瘤细胞增殖的机制。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。