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There are many studies concerning that the genetic variations of UGT1A1 are associated with unconjugated hyperbilirubinemia, and irinotecan-induced adverse drug reactions; however, there is no studies to investigate the association of liver injury during antituberculosis therapy and UGT1A1 genetic variations.

UGT1A1基因变异与未结合型高胆红素血症,及irinotecan诱发之药物不良反应有相关,但尚未有UGT1A1基因变异的族群,在接受抗结核药物治疗期间发生肝损伤之研究。

Objective To investigate the interaction between 5-HT2A receptor gene and MAOA gene in unipolar depressive patients.

目的 探讨单相抑郁症病因中5-HT2A受体基因与MAOA型基因的相互影响。

The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.

结果显示:先证者尿检呈强阳性;其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变,为半合子突变,而其母、其姐为杂合突变;正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变;不同物种氨基酸序列的同源性比对显示: c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守;酶活性测定的结果显示:先证者的IDS酶活性仅为2.3 nmol/4 h/mL,大大低于正常值,而其父的为641.9 nmol/4 h/mL,其母的血浆酶活性为95.8 nmol/ 4h/mL,其姐的为103.2 nmol/4 h/mL。

The CryCI gene and essential regulation elements cut from the plasmid pGF4ABC were inserted into yeast expression vector pPIC9K and plant expression vector pBI 121.1, so we got the secretive yeast expression plasmid pPIC9KBC and plant high-efficiency expression plasmid pGBIF4ABC, Fifty-two His+Muts transformants were obtained after the expression vector pPIC9KBC was introduced into Pichia pasoris, KM7I strain, by electroporation.

将pGF4ABC上的融合杀虫基因CryCI分别克隆到酵母表达载体pPIC9K和植物表达载体pBI121.1上,构建成融合杀虫基因的分泌型酵母表达载体pPIC9KBC和高效植物表达载体pGBIF4ABC。

It was known that ORF of SeMNPV egt was 1572bp long and encodes 523 amino acids through DNAstar software analysis. TATA boxes lies at 48~53 and 68~72bp of the 5'-nonencoding region upstream of a translational start site. Comparing with seven kinds of nuclear polyhedrosis virus and one kind of granulosis virus, it indicated that the SeMNPV egt gene is the highest match the Agrotis setegum nuclear polyhedrosis virus.It has 75% nucleotides and 79% amino acid identity.

采用DNAstar软件分析,与7种核多角体病毒及1种颗粒体病毒egt基因的同源性比较显示,SeMNPV egt基因与核多角体病毒egt序列同源性较高,其中与黄地老虎核型多角体病毒同源性最高,核苷酸和氨基酸序列同源性分别为75%和79%。

The pig major histocompatibility complex class Ⅱ antigens have been known to exhibit a different degree of allelic polymorphism.

重点介绍了SLAⅡ类基因的RFLP基因分型及其多态性研究。

Flower sex expression of monoecious lines (ATA-A and ATA-B) and andromonoecious lines and their offsprings (F1 and F2 and BC1) was investigated. Results showed that the flower expression character of that monoecious lines of melon was controlled by a pair of genes at one single locus,and the Monoecious character was controlled by the dominant gene.

通过对甜瓜单性花系ATA-A、ATA-B及他们的杂交后代F1、F2、BC1的花性型分离情况进行统计分析研究后认为,甜瓜单性花性状由一对基因控制的质量性状,单性花性状由显性基因所控制,其遗传符合孟德尔遗传规律。

Recently,numerous studies proved that each member of secreted aspartyl proteinase have different requirements in temperature and pH,with develop of gene mutation technology further cleared relation between gene expression regulation and organism′s infection sites and state,as well as transcription activation factor effect on expression of each member of secreted aspartyl proteinase.

近年来,大量研究证实了分泌型天冬氨酸蛋白酶各成员对温度、pH值等培养方面有不同的要求,基因突变技术的发展,进一步明确了基因表达调控与机体感染部位、感染状态之间的关系及转录活化因子在蛋白酶各成员表达中的作用。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组,并将阳性重组质粒,应用M13测序系统对产物进行DNA序列分析,并与MPM-129株P1基因核苷酸进行同源性比较,发现有4个位置的碱基发生了变化,其同源性为98.8%,证实了PCR所扩增DNA片段的准确性和特异性,同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异,将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针,杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应,再用BCIP和NBT呈色,制备MP DNA探针,鉴定所扩增片段的特异性,与同位素探针比较,Dig探针不受半衰期限制,可反复使用,而且价格低廉,值得推广使用。

Suppression subtractive hybridization was used between virulent strain HA9801 and avirulent strain 12. Five fragments of putative virulent genes were found by this method.

并采用抑制性差减杂交的方法对2型毒力菌株所携带的差异基因序列进行了研究,发现了5个新的可能的毒力基因。

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