基因型

Under different stresses, we tested the expression of citrate synthase gene in rapeseed leaf by using semi-quantitative PCR. The expression of citrate synthase gene had no obvious change in stresses of salt, dark, high illumination, while was increased at different time in treatments of water logging, drought, IAA, and 6-BA. Interestingly the effect of ABA was contrary to that of IAA. In the treatment of sclerotium blight, the expression of citrate synthase gene was depressed. There was a saddle curve of citrate synthase gene expression in the treatment of gibberellin.

对油菜幼苗进行植物生长调节物质、高温和低温、强光照和弱光照、盐、菌核病、干旱和水渍等处理，采用半定量PCR法对油菜叶片柠檬酸合酶基因的表达模式进行检测，发现在盐胁迫、暗光和强光的处理下，柠檬酸合酶基因的表达基本没有变化；在水渍、干旱、IAA和6-BA胁迫下，其表达有所升高，但出现峰值的时间不同，ABA对表达模式的影响与IAA相反，感染菌核病后其表达降低；对GA3的应答呈鞍型。

Objective To observe the influence of gene transfection of inducible nitric oxide synthase to the transplanted human laryngeal squamous carcinoma in nude mice, to investigate the expression and the correlayion of iNOS and p53, and to proke into the possible apoptosis mechanism. Methods Transfect the gene of iNOS to Hep-2 human laryngeal cancer cell, sieve out positive clone cell and amplify.

目的 观察诱生型一氧化氮合酶(inducible nitric oxide synthase， iNOS)基因转染对人喉鳞癌细胞裸鼠移植瘤生长的影响，研究在体人喉鳞癌细胞中iNOS与突变型p53基因的表达，探讨二者的相关性及促进肿瘤细胞凋亡的机制。

In this study,we attemped to use the same strain samples to compare the above three methods on the relationship between DNA patterns and geographic origins and clinical manifestations,to determine which is the best method on the genetic typing of S.schenckii. Also,we attempted to screen the highest specific and sensitive probe and primers from which had been reported of S.schenckii to lay the foudation for the rapid and correct diagnosis of sporotrichosis.

本研究旨在以相同的孢子丝菌菌株为样本，对以上3种方法进行比较，以确定在基因分型上哪种方法相关性更好、分辨力更强，并筛选出对孢子丝菌更敏感和更特异的探针和引物，为从基因水平上快速、准确诊断孢子丝菌病奠定基础。

Aim: To research oral streptococci strain gene identification and compare their difference.

目的：探讨口腔链球菌基因分型，比较细菌相互间基因差异。

Results there 15 alleles and 25 genotypes were found in li ethmic subpopulation on hainan island.

结果 在3个y-str基因座，3个支系人群共发现15个等位基因，25种单倍型。

Objective To investigate the suppression effect of tumor suppressor gene p53 gene in lung adenocarcinoma cell lines.

目的探讨野生型p53基因及其表达产物p53蛋白在恶性肿瘤基因治疗中的作用。

Objective To construct a recombinant eukaryotic expressing vector pcDNA3.1-Myc-His B/t-PA including functional region of human tissue-type plasminogen activator gene to provide a basis for further study on the therapy of thrombotic disease.

目的 构建人组织型纤溶酶原激活因子基因的新型真核表达载体，为血栓性疾病的基因治疗奠定基础。

Rht-Blc in "Tom Thumb", a native wheat germplasm originated in Tibert, China, is a semi-dominant and GA insensitive gene. It has stronger effect on plant height than Rht-Blb and Rht-Dlb and could be the cause of lower activity of a-amylase in the wheat grain of Rht-Blc-carrying lines.

小麦矮杆基因Rht-B1c来源于我国地方品种西藏大拇指矮，表现为半显性、GA不敏感的矮化表型，携Rht-B1c基因的籽粒内α-淀粉酶活性低、抗穗发芽。

For investigating the linkage group to which the semidwarf gene sd-t belongs,the crosses between Aitaiyin 2(ATY2),a variety of sd-t carrier in indica rice,and marker stocks as well as the trisomic lines of IR36 were made.

以籼型标志基因系和IR36三体为工具材料，通过杂交研究了籼稻矮秆材料矮泰引-2所携半矮秆基因sd-t在染色体上的位置。

For investigating the linkage group to which the semidwarf gene sdn belongs, the cross between Yizhi\|lashuangai, a material of sdn carrier of Indica rice, and marker stocks as well as the trisomic lines of IR36 were made.

以籼型标志基因系和IR3 6三体为工具材料，通过杂交研究籼稻矮秆材料一枝腊双矮所携半矮秆基因sdn在染色体上的位置。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。