基因型

Six pairs of oligonucleotide primers were designed to amplify and sequence scarlet gene of mutant stbr flies which were obtained by outcross between Bar and wild type flies.

设计了6对引物对本实验室由棒眼果蝇Bar与野生型果蝇杂交所产生的亮红眼突变型果蝇stbr的scarlet基因进行了测序，结果在scarlet基因的第4外显子检测到了一个7.5kb的插入，进一步的测序证实此7.5kb插入为逆转座子412元件。

Genotyping results displayed an overrepresentation of the T allele in cases as compared with controls 48% vs.

基因分型结果显示实验组与对照组相，T等位基因的过度表达48% vs。

Domenstic and abroad academicians have disclosed the influence mechanism of these factors from different aspects, including the gene polymorphism and gene mutation in genetics, which are a susceptible factor for cardiovascular complication in type 2 diabetes patients; the dysfunction of endotheliocyte, the metabolic disorder of lipid, and dysfunction of vascular smooth muscle, which can lead to the formation of arteriosclerosis;the formation of immune complex,which can also accelerate the destruction of artery paries;the sctivation of protein kinase C and the dysfunction of platelet, which may ultimately result in the formation of thrombus and influence the function of cadiac muscle cells.

其中主要包括：遗传学方面的基因多态性和基因突变，二者均是2型糖尿病合并心血管疾病的一个易感因素，内皮细胞功能障碍、脂质代谢异常及血管平滑肌细胞功能异常均可导致动脉粥样硬化的形成；免疫复合物的形成可加速动脉壁的破坏；蛋白激酶C的激活及血小板功能受损最终导致血栓形成并影响心肌细胞的功能。

BACKGROUND: Asthma is a complex phenotype that is influenced by both genetic and environmental factors.

背景：哮喘具有较为复杂的基因分型，受到基因和环境因素的双重影响。

We previously reported that many genes involved in tetrapod tissue mineralization arose from a common ancestor and constitute the secretory calcium-binding phosphoprotein gene family.

我们先前的研究证实，许多与四脚动物矿化有关的基因均来自于一个共同的祖先，组成了分泌型钙结合磷蛋白基因家族。

The major gene of CTXΦ receptor Tcp (toxin-coregulated pilus), tcpA, was detected by polymerase chain reaction analysis.

然而在本实验室研究中，于不含ctxAB基因的El Tor型霍乱弧菌中发现部分菌株含有RS基因同源序列，为此对这些菌株作CTXΦ基因组的进一步研究。

The total RNA was isolated from pokeweed leaves using the method of guanidine isothiocyante and used as template for amplification of the deleted mutant pokeweed antiviral protein gene by RT-PCR and then the amplified fragment was cloned into secreted expression pPIC9K vector to form pPIC9K-p, then the vector was transferred into Pachia pastoris GS115 strain.

从美洲商陆叶片中通过异硫氰酸胍法提取出了总RNA，经RT-PCR扩增出缺失突变型抗病毒蛋白PAP基因，将该基因克隆于分泌型真核表达载体pPIC9K，然后导入毕赤酵母菌株GS115细胞。

The baculovirus expression system, utilizing the strong polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus , has become an important tool for protein production in the field of molecular biology.

由于核型多角体杆状病毒的多角体蛋白不是病毒复制必需的蛋白，利用转录多角体蛋白基因的强启动子可以高效表达外源蛋白，包括原核和真核基因编码的蛋白。

The research progress of redifferentiation of dedifferentiated thyroid carcinoma by up-regulation of thyroid iodide-metabolizing genes expression and suppression of BRAF mutation is reviewed in this paper.

文章就通过上调甲状腺碘代谢相关基因表达和抑制BRAF基因突变而使失分化型甲状腺癌再分化的研究进展作一综述。

The advances and existing problems concerning in genetics especially on the sex-inheritance and tolerance to papaya ring spot virus, the genetic improvement (cross breeding, mutation breeding, introduction breeding, culture in vitro, molecular marker assistant selection and bioengineering) on papaya are reviewed; especially on the sex-inheritance, distant hybridization and embryo culture, and breeding for disease resistance to PRSV with gene engineering are discussed in detail.

就番木瓜的株性和抗病性等性状遗传、杂交育种、人工诱变育种、离体培养、分子标记及基因工程育种等现代生物新技术育种方面的研究进展及相关问题进行了评述，重点综述了番木瓜株性遗传、远缘杂交与胚胎学、基因工程在抗环斑型花叶病毒病品种培育及分子标记辅助选择育种应用的研究进展，并分析了今后番木瓜育种的发展趋势。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。