基因型

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

Since the Km〓 gene of pCS4 and pCS11 was derived from the mesophile plasmid pUB110 and coded for a thermolabile protain, it conferred Km〓 phenotype on the host cell at 48℃ only. Mutant Km〓 gene was obtained by selecting for Km〓 phenotype at elevated growth temperature. In this way improved vectors pCS5 and pCS12 were obtained, which can be used at 65℃ by Km selection.

利用提高培养温度后选择卡那霉素抗性表型的方法，从编码不耐热蛋白质的卡那霉素抗性基因出发，得到了提高其耐热性的突变的卡那霉素抗性基因，从而改造了CS4和pCS11，得到了适用温度达65℃的改进的载体质粒pCS5和pCS12。

MGD data content includes comprehensive characterization of genes and their functions, standardized descriptions of mouse phenotypes, extensive integration of DNA and protein sequence data, normalized representation of genome and genome variant information including comparative data on mammalian genes.

MGD数据内容包括了基因和它们功能的综合特征，小鼠表型的标准化的描述，DNA和蛋白质序列数据的广泛整合，包括与哺乳动物基因的比较数据的基因组和基因组变异信息的归一化的表征。

Lahn and his colleagues looked at the abnormal spindle-like microcephaly associated gene, which scientists had previously identified as a key player in brain development.

拉恩和他的同事对"异常纺锤型小脑畸形症相关"基因进行了观察。科学家们早就认定ASPM基因是推动大脑发展的一个决定性因素。

There is an agreement that autosomal recessive juvenile parkinsonism is a monogenic disease caused by mutation in parkin gene.

一致的观念认为常染色体隐性遗传性青少年型帕金森综合症是parkin基因突变引起的一种单基因遗传病。

Scientists found that the montane vole had a short version of the vasopressin receptor gene, and the monogamous one had a long version of it.

科学家们发现，那些山地的田鼠有一个较短样式的垂体后叶荷尔蒙接收基因，而那些单配型的田鼠的垂体后叶荷尔蒙接收基因样式较长。

The neurofibromatosis type 2 (NF2) tumor suppressor is the only gene known to be frequently involved in early development of meningiomas.

多发性神经纤维瘤2型(NF2)肿瘤抑制基因是已知唯一的经常出现在脑膜瘤早期发展过程中基因。

Objective:To understand the neutrophil antigen gene polymorphism in the Hui nationality in North west China by typing of NA gene.

目的：通过NA基因分型，了解中国西北地区回族人群NA基因多态性。

In our previous STM studies we found that the disruption of the hns gene encoding a histone-like nucleoid structuring protein could enhance biofilm formation of APP.

我们在筛选APP转座突变体库时，发现hns基因缺失突变可显著增强APP血清1型生物被膜的形成，该基因编码一种DNA结合蛋白。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。