基因型

The phylogenetic analysis of 22 ND4L sequences of Phocidae from GenBank showed that there are two clades (which belong to Northern Group and Southern Group) supported by relatively high bootstrap value and there are more closed relationship between spotted seal and harbor seal.17 haplotypes with 717bp were obtained from the mtDNA control region, adjacent proline and a portion of the threonine tRNA genes of 46 individuals.

利用从GenBank中下载的22个海豹科动物的ND4L基因序列的系统进化分析表明，海豹科动物分为南半球和北半球两大类群，斑海豹与港海豹为北半球种类，亲缘关系最近。从46个辽东湾斑海豹线粒体DNA控制区和苏氨酸及脯氨酸tRNA基因717bp片段的测序，得到17个单倍型。

The mating type gene is inherit decision factor of compatibility with the performance type,P.ostreatus belongs to sexual reproduction heterothallism,tetrapolarity,Namely the colony which is consist of single spore mycelium will self-sterility,If conjugate sex gene with all for incompatibility,then crossing can dicaryotization and form sporophore.

交配型基因是性亲和表现型遗传决定因子，平菇为有性生殖异宗配合四极性类型，即单孢形成的菌落自体不育，不同单核菌丝在配对基因皆为杂交结合时才能双核化，形成子实体。

Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P ＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

These variations may have some effect on gene structures and gene expression, and then contribute to intraspecific phenotypic diversities.

在物理图提供的序列基础上，对于籼粳稻两个主要栽培稻亚种进行了比较基因组分析，在2.3Mb对应区段中发现了两个亚种在基因组成和顺序上存在的广泛的微共线性，两者之间的差异主要由插入/缺失和单核苷酸多态性造成，这些差异可能影响基因的结构和表达，从而对种间表型差异产生影响。

Knockout and knockdown animals and/or cells can be closely examined for changes in phenotype.

之后可以对基因敲除和基因敲低动物和/或细胞的表型变化进行仔细检查。

Aim To observe changes of peroxisome proliferator activated receptor γ mRNA, ATP-binding cassette transporter A1 (ABCA1) mRNA and CD36 mRNA in foam cells which come from the peritoneal macrophage of apolipoprotein E knock out mice with giant knotweed rhizome, hawthorn and both of them, and discuss possible mechanism of anti-atherosclerosis on gene level.

目的 观察中药虎杖、山楂配伍对载脂蛋白E基因敲除小鼠巨噬细胞源性泡沫细胞内过氧化体增殖物激活型受体γ、三磷酸腺苷结合盒转运子A1及CD36 mRNA表达的影响，从基因水平探讨虎杖和山楂配伍对动脉粥样硬化泡沫细胞形成的干预机制。

Of the 150 patients screened for KRAS mutations, 26 (17.3%) patients were identified.

本研究中并未发现UGT1A7 基因多型性与KRAS 基因突变的发生具有显著之相关性。

Objective: To explore the relationship between various mitochondrial DNA tRNA Leu gene/ND-1 gene mutations and type 2 diabetes. Methods: Case-control study was designed.

目的：探讨DNA tRNA Leu基因及ND-l基因3316G/A、3394T/C等4个突变位点与2型糖尿病的关系。

The purposes of this experiment were to investigate the segregation of coat color in swine at vary mating types between Taoyuan and Duroc, and to understand that uniform black coat color of T was weather dominant or not to uniform red color of D, the adjusted Chi-square(χ2 ) test was used to determine the goodness of fit for Mendel's law of segregation.

本试验的目的是调查桃园种与杜洛克猪只各种配种型态产生后代之肤色分离情形，并以矫正之卡方测定进行遗传分离适合度检定，以了解桃园种全黑色肤色的基因与杜洛克全红色肤色基因有无显隐性关系。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。