基因型

To elucidate the molecular mechanisms underlying the neural plasticity after CNS injury, the gene expression profile in the rat hippocampus following perforant path transections was explored by several methods including custom differential screening, custom cDNA array and cDNA microarray. Of 2300 cDNA clones from low-abundance rat brain cDNA bank, 6 were identified as differentially expressed genes/ESTs in the hippocampus 35 days after lesion, in which none was confirmed by Northern blot. Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated.

中文题名去传入海马的差异表达基因筛选和Cystatin C表达副题名外文题名 Screening of the differentially expressed genes and cystatin C expression in the deafferented hippocampus 论文作者应国新导师周长福研究员学科专业神经生物学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数121页关键词海马星型胶质细胞 Cystatin C 基因表达去神经海马馆藏号BSLW /2003 /Q42 /27 为了阐明中枢神经系统损伤后神经可塑性的分子机制，本实验探索了多种方法，包括传统差异筛库、传统cDNA array和cDNA microarray，对穿通纤维切断后大鼠海马的基因表达图谱进行了研究。

The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12 000 independent transformant lines were acquired by the above method, from which two mutants related to plant defense response and development, asr1 (auxin and .salicylic acid responsive 1.) and dai1 (brought tolerant and obscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSK1015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asr1(auxin and salicylic acid responsive 1)和dai1(drought tolerant and abscisic acid insensitive 1)。

The main work of this dissertation is constructing Arabidopsis gain of function mutants pool, screening mutants related to plant defense response, cloning the genes and studying their functions. The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12000 independent transformant lines were acquired by the above method from which two mutants related to plant defense response and development, asr1 (auxin and salicylic acid responsive 1) and dai1 (drought tolerant and abscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSKI015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asrl(auxin and salicylic acid responsive l)和dail(dought tolerant and abscisic acidinsensitive l)。

To understand whether there is gene reassortment recently, we also analyzed the evolutionary relationship of these isolates. It appears that no HA/NA reassortment was found.

想了解这段时间内是否出现基因重组，我们也分析了这些分离株NA及HA基因的演化关系，结果显示这段时间内的A型流感病毒在HA及NA基因之间没有出现互换的现象。

Objective To establish a sensitive and specific gene probe method for detection of Staphylococcal enterotoxin B gene.

目的 建立一种灵敏度高、特异性好的DNA 探针检测葡萄球菌肠毒素B 基因方法。方法用聚合酶链反应扩增的方法进行葡萄球菌B 型肠毒素基因探针生物素标记，用制备好的基因探针，进行斑点杂交，对葡萄球菌肠毒素10 株标准株和39 株临床株分别进行了检测。

During the past 3 years, 2 independent experiments of sperm-mediated gene transfer in domestic silkworms were carried out respectively in 2000 and 2002 with the method direct injection of copulation thecae.

在同源重组绿色荧光蛋白表达载体pG350的基础上，以pMD18-T为质粒载体，用egfp基因替换gfp基因，构建了家蚕核型多角体病毒早期即刻蛋白基因启动子BmNPV-IE控制下的增强型绿色荧光蛋白表达载体pMD-Fib-IE-EGFP。

A search for genes expressed in each of the major cell types in the brain reealed a number of preiously uncharacterized genes that each appear to be specific to one cell type.

一篇关于基因表达的文章提到，脑中有很多之前未做特异性标记的基因，但这些基因都一对一的对应着一种细胞型。

Lysine content, kernel phenotype, zein accumulation and opaque-2 gene sequence of four maize inbred lines (CA335, CA339, Lu2548 and Qi205) were investigated in this study. These QPM inbred lines as the donor of Opaque-2 mutant gene (o2) respectively, were backcrossed to introgression o2 into elite maize inbred lines based on marker-assisted.

对CA335、CA339、山东2548和齐205等4个国内常用优质蛋白玉米自交系的赖氨酸含量、籽粒表型、醇溶蛋白积累及opaque-2基因的序列进行了分析；对不同自交系作为Opaque-2突变基因（o2）基因供体的分子标记选择效果进行了初步的比较。

This will cause the final Pareto optimal frontier perform feature subsets with many features erasing and less improvement of classification rate. In order to improve the accuracy of classification, it is necessary to avoid the over trimming of the features. In this study, we propose a constraint based niched Pareto genetic algorithm to adjust the thread of search.

由於在实际问题中，提升分类准确率，常常会比大幅删减使用特徵数来得重要，本研究提出限制型利基柏拉图基因演算法，透过在基因演算法的挑选过程中，增加一准确率限制的手段，调整基因演算法之搜寻方向，以避免特徵的过度删减，并确保分类准确率的提升。

In order to develop a new path for gene mapping of fish, the digoxigenin and tritium labeled in situ hybridization techniques combined with multiple banding analysis on the pachytene bivalents of M. albus were studied using the wheat ribosomal RNA genes, pig growth hormone gene and MaSoxl (M. albus SRY boxl) DNA segment as the probes. Moreover, the techniques of random primed in situ DNA synthesis were also developed on the bivalents of M.

以两种异源基因和Ma Soxl为探针，采用地高辛或氟标记的原位杂交技术，结合点渍法和DNA印渍法分析，率先在黄鳝减数分裂二价体上开展基因定位研究，并与多重带带型分析相结合，以期为构建黄鳝染色体框架图奠定初步基础，为迅速改变鱼类基因定位研究的落后局面而开辟新的途径。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。