基因型

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

Such information includes not only the structural and biochemicalproperties of the gene products and their roles in specific tissues, but also theloss-of-function phenotypes.

这种功能信息既包含该基因表达的蛋白产物的结构和生化特性方面的信息，以及该基因表达产物在具体的组织所具有功能的信息，又包含丧失该基因功能的生物体的表型信息。

The analysis of the mutants of amrA and amkA showed that the production of rifamycin SV had decreased about 10%-20%.

用双交换法在地中海拟无枝菌酸菌U32染色体上插入阿泊拉霉素抗性基因，成功地构建了了amrA，amkA和amrB基因的突变株，其中amrA，amkA基因突变株的菌丝形态与野生型U32之间有较明显的差别，在菌丝的末端形成卷曲或弯曲。

Objective To construct spinocerebellar ataxia 3 (SCA3) eukarotic expression vector with en hanced green fluorescent protein as report gene and to observe its expression in PC12 cells.

目的 构建以增强型绿色荧光蛋白作为报告基因、带有脊髓小脑性共济失调3型(SCA3)基因的真核细胞表达载体，并转染PC12细胞，观察外源基因的表达情况。

Methods p53 codon 72 were genotyped with pcr-rflp technique for 156 zhuang healthily long-living people (aged 90～105, mean 93.2 years, longevous group) and 142 healthy controls (20～72, mean 34.8 years, control group) from bama area to analyze the polymorphic genotype frequencies.

应用pcr-rflp技术对巴马156例（年龄90～105岁，平均93.2岁，长寿组）健康壮族长寿老人和142例健康壮族成年人（年龄20～72岁，平均34.8岁，对照组）的p53基因进行分型，了解这些基因的多态性，分析该基因频率在长寿组和对照组之间的差异及其与长寿的关系。

The morphologic and immunohistochemical discrepancies between this intriguing melanotic tumor and other documented renal cell carcinomas bearing identical PSF-TFE3 gene fusion may suggest melanotic Xp11 translocation renal cancer is a distinct entity among the MiT/TFE family neoplasms.

与同样发生PSF-TFE3基因融合的其他已知肾细胞癌相较，此例的形态、免疫表型差异可以说明，黑色素性Xp11基因易位肾癌是MiT/TFE基因家族肿瘤中一个独特的实体。

In recent years,mutations of gene encoding gamma-aminobutyric acid A-receptor γ2 subunit (GABRG2) were founded linked to many kinds of epilepsy syndromes such as Febrile Seizure, Generalized Epilepsy with Febrile Seizure plus, Childhood Absence Epilepy, Severe Myoclonic Epilepsy of Infancy.

近年来许多学者通过体外扩增基因等研究发现在许多种癫痫综合征患者种都存在编码γ-氨基丁酸A型受体γ亚单位的基因异常，进而提出了GABRG2基因异常与多种癫痫的发病有关。

So far transgenic knockout models of AQP1, AQP3, AQP4 and AQP5, and a knock-in model introducing a point mutation (T126M) that causes autosomal recessive nephrogenic diabetes insipidus in human have been successfully established. Significant progresses have been made in characterizing the physiological functions of these AQPs by systematic mouse phenotype studies.

目前AQP1、3、4、5基因敲除和AQP2基因点突变的基因敲入小鼠模型（模拟人类常染色体隐性遗传尿崩症）已成功建立并广泛用于表型研究，在AQP水通道蛋白生理功能方面获得许多重要进展。

These results indicated that the genetic variation within exon 2 appeared to have largely arisen by gene recombination and balancing selection. Alignment of the deduced amino acid sequences of the β1 domain coded by exon 2 revealed 6 synonymous mutations and 27 nonsynonymous substitutions at the 33 disparate sites.

对B-LBⅡ等位基因第二外显子所编码的B-LBⅡ分子β1结构域氨基酸序列比对发现，31个B-LBⅡ新等位基因属于26个等位基因主型；在β1结构域氨基酸序列的33个变异位点上，存在6个同义替换和27个错义替换。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。