基因型

This result indicates that WT1 gene plays an important role in differentiation and development of fetal kidney and may be the factor that promotes metanephric blastemal cell to differentiate into epithelial cell.

结果显示小胎龄肾组织中WT1蛋白在胚基细胞和幼稚肾小球细胞核表达而大胎龄组肾组织中WT1在肾小管细胞胞浆表达，阳性率分别为57.1％(8/14)和46.2%(6/13)，提示WT1基因在胚胎肾分化发育的过程中起着重要作用，WT1蛋白可能是促进后肾胚基细胞向上皮细胞分化的调控因子，其表达在时间上和空间上都受到严格的调控，WT1的表达异常可能导致胚基细胞分化停滞。17例肾母细胞瘤WT1蛋白表达阳性率为41.2%(7/17)，阳性部位在胚基型和上皮型肿瘤细胞核，表达部位和阳性率与早期胚胎肾相似，其中间质型肾母细胞瘤均为阴性，胚基型和上皮型肾母细胞瘤阳性率70%(7/10)，两组间阳性率有显著差异。

Our study designed two pairs of specific primers and cloned two molecular weight of 63ku from Schizaphis graminum, and Rhopalosiphum maize utilizing PCR technique and have done the prokaryotic expression, the main results are as follows:(1)Amplified the Buchnera groEL of Schizaphis graminum, and Rhopalosiphum maize Yangling Biotype at first time all over the world ,and sequenced the full lenghth gene.

本研究设计了特异性引物，利用PCR技术从玉米蚜和麦二叉蚜中克隆出蚜虫Buchnera groEL基因并进行原核表达研究，主要结果如下：(1)从杨凌生物型玉米蚜和麦二叉蚜中在国内外首先扩增出Buchnera groEL基因，测定了全长基因序列，并对相关的同源基因核苷酸进行了比较。

compared with the classic rabies virus, the strains shared the higher degree of homology with the street viruses obtained from thailand and philippines than other known strains especially that rabies virus isolated from chiroptera in america.

结果 同源性分析表明， 4株湖南街毒p和m基因核苷酸同源性分别为97.3%～99.4%和98.4%～99.8%；与其它i型狂犬病病毒和疫苗株核苷酸同源性比较，p基因分别为78.4%～90.2%和81.8%～86.8%；m基因的分别为81.8%～92.1%和84.7%～90.6%。4株野毒p和m基因氨基酸同源性分别为98.0%～99.3%和98.5%～99.0%。

It has been thought that the complicated gene-gene interaction, heterogeneity of clinical presentation and numerous farraginous experimental data make it more difficult to draw a firm conclusion with genetic research work.

这主要是因为精神分裂症临床分型复杂，症状多样，若将不同类型的患者混杂起来研究将难以对这种异质性疾病作出可信的结论；同时该病作为多基因病的一种，其发病是由许多微效基因共同作用决定的，若孤立地研究某个候选基因或候选染色体区域与疾病的关系，也难以得出满意的结果；而且在研究结果的分析中，某个基因对发病的微小贡献将被&淹没&在巨大的数据信息中，难以提取。

The main component include promoter sequence (5' flank control rigon of goat P - lacto globulin gene of goat),foreign gene,Marker gene and genome of defective retrovirus.

其中主要构建元件包括：启动子序列(山羊β-乳球蛋白基因5'端调控区)；外源基因(人乳铁蛋白基因cDNA)；标记基因；缺陷型逆转录病毒基因组。

Our research utilized two cell lines carrying a bisatellite microchromosome originated from two normal phenotypic families found by Professor Jiahui Xia to make the BM become a new vector for gene therapy. The research will finally establish a novel vector that can be steadily transmitted, has enough capacity to carry foreign genes, has no harm to human being, has no immunogenecity and is easy to transfer. This research can provide brand new vector tool for gene therapy of human hereditary diseases and tumors.

本研究利用夏家辉教授发现的2个带有双随体小染色体而表型正常的家系所建立的细胞株作为研究材料，用BM作为基因治疗新载体，以求最终建立一种能稳定遗传、携带外源基因容量足够大、对人体无害、无免疫原性和易于基因转移的新型载体，为人类遗传病和肿瘤的基因治疗提供崭新的载体工具。

Then we transformed mTn-lacZ/LEU2 transposon library into the YES2MTM1 strain. Transformants lost the plasmid overexpressing MTM1 after 5-Fluoroorotic acid treatment. We picked up the yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and some transposon insertion and identified the insertion sites.

因MTM1基因的缺失造成的损伤不可逆，直接转入文库无法筛选得到MTM1 基因缺失表型相关基因，本研究利用外源MTM1基因菌株和mTn-lacZ/LEU2酿酒酵母转座子文库进行筛选，寻找能挽救mtm1突变体生长缺陷的转座子插入位点。

The nosogenetic loci about type 2 diabetes mellitus and bipolar disorders were studied in order to provide more scientific bases for the prevention and cure of diseases.

目的：探索人类复杂疾病相关基因位点研究统计分析的合适模型，为复杂疾病致病基因位点的探查提供较为敏感的统计分析方法；探讨线粒体基因突变2型糖尿病与双相情感性精神障碍的易感基因位点，为疾病的防治提供更为科学的依据。

Objectives: Exploring appropriate model on statistical analysis for genetic loci of complex human disease and providing the sensitive methods of statistical analysis for study of the nosogenetic loci. The nosogenetic loci about type 2 diabetes mellitus and bipolar disorders were studied in order to provide more scientific bases for the prevention and cure of diseases.

目的：探索人类复杂疾病相关基因位点研究统计分析的合适模型，为复杂疾病致病基因位点的探查提供较为敏感的统计分析方法；探讨线粒体基因突变2型糖尿病与双相情感性精神障碍的易感基因位点，为疾病的防治提供更为科学的依据。

Methods After the wide type NCI-H446 cells and the three kinds of NCI-H446 cells transfected by three kinds of IL-15 genes respectively （Cmp transfected by the IL-15 maturation peptide gene, Cp transfected by prototypic IL-15 gene, and Csp transfected by modified IL-15 gene whose signal peptide was replaced with the IL-2 signal peptide） were treated by mitomycin, the cells （named MCw, MCmp, MCp, and MCsp, respectively） were used as stimulator cells to stimulate PBMC from healthy volunteers.

野生型NCI-H446细胞和被3种IL-15基因分别转染的3种NCI-H446细胞（Cmp：被IL-15成熟肽基因转染；Cp：被原型IL-15基因转染；Csp：被信号肽换为IL-2信号肽的改型IL-15基因转染），用丝裂霉素处理后（分别名为MCw、MCmp、MCp和MCsp）作为刺激细胞刺激健康志愿者的PBMC。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。