基因型

Methods Fifty pairs of fresh colorectal cancer and homologous normal tissues were genotyped with Identifiler Kit and the mutations generated in cancer tissues were determined. The mutation rates, the numbers of locus matched without identical allele (A0), 1 identical allele (A1), or 2 identical alleles (A2) and the number of total identical alleles IA(subscript n were calculated. Frequency distributions of A0, A1, A2 and IA were compared among CR-N group, unrelated individual pairs and full sibling pairs. Discrimination functions were established for individual identification from tumor tissues with discriminatory analysis.

用Identifiler系统对50对新鲜结直肠癌组织及其身源正常组织组进行STR分型，计算CR-N组中变异STR基因座及全不同基因座数(A0)、半相同基因座数(A1)、全相同基因座数(A2)和共有等位基因数IA(下标 n，比较CR-N组、无关个体对组和全同胞对组中上述参数的分布差异，通过判别分析建立判别函数。

The transform of phenotype of VSMC: The mRNA expressions of SM22α-a notate gene of contractile VSM-were all detected on normal aortas,but decreased normally when the aortas were destroyed and cultured in vitro for 10 days,especially in the serum groups,whose SM22αmRNA could not be detected by RT-PCR.Here,BQ123 can increased their expressions.This demonstrated that serum culture and the secretion of ET-1 can produce the transform of phenotype from contractile to synthesize type.

血管平滑肌表型的转化：RT-PCR检测发现，血管平滑肌收缩表型标志基因SM22α在正常血管平滑肌中都有表达，但在内皮损伤后体外培养10天的血管表达明显减弱，其中血清培养组血管基本不表达，加内皮素受体阻断剂BQ123后，各组SM22α的表达均有所上调，这表明内皮素分泌和血清培养促进了血管平滑肌的表型由收缩型向分泌型的转化。

We isolated nine cuticle protein genes, two peptidase genes, and various tubulin isotypes through the EST catalog, and one cuticle protein gene and two protease genes through the microarray analysis.

我们经由EST资料库找到9个表皮蛋白基因，2个胜肽脢基因，和多种的同功型微管蛋白，以及利用微阵列分析找到1个表皮蛋白基因和2个蛋白脢基因。

The major gene heritability and polygene heritability were 64.17% and 14.76% respectively, which showed major genes played major role in seed setting rate.

在主基因作用的效应中，加性效应和显性效应各占主基因效应的50％；主基因的遗传率较高，分别为56.97％和44.26％；多基因的遗传率很低，分别为1.24％和3.05％；环境对结实率的影响显著，其方差占总表型方差的43.07％和52.99％。

Thus, if height is controlled by two genes, A and B, and tall and short forms are determined by dominant and recessive alleles respectively, then the cross Aabb × aaBB would give an F1 AaBb, containing both dominant genes for tallness.

假设，高度性状是由两个基因 A 和 B 共同控制，且高与矮两种表型分别为等位基因中的显性和隐性基因所决定，那么， Aabb × aaBB 杂交的结果是 F1代为 AaBb ，含有决定高性状的两个显性基因。

The total 48 Haemophilus parasuis strains isolated from Guangdong province in 2007-2008 were serotyped by Indirect Haemagglutination Assay, and respectively belonged to 7 serotypes, 5 serotype with 23.68%.

采用肠杆菌基因间重复序列-PCR（Enterobacterial repetitive intergenic consensus-PCR）对保存的2007～2008年广东地区发病猪群中分离的48株副猪嗜血杆菌进行了分析，用间接血凝进行血清分型，PHYLIP-3.68软件对电泳图谱进行基因分型及遗传关系的分析。

The expression of some oncogenes in the cells was examined by means of chromosomal pattern analysis and immunohistochemistry.

应用染色体核型分析和免疫组化方法，分析原代细胞核型以及癌基因与抑癌基因的表达。

Methods:The difference of PTH gene and protein expression in parathyroid gland and thymus were compared between PTH knock-out mice 1αase knock-out mice and their wild-type littermates at 2 weeks of ages by RT-PCR,real-time PCR and immunohistohemistry.

real-time PCR和免疫组织化学的方法比较分析同窝2周龄PTH基因敲除纯合子和野生型小鼠以及1-α羟化酶基因敲除纯合子[1αase-/-]和野生型小鼠的甲状旁腺和胸腺的PTH表达差异？

More and more HLA genotyping methods have been developed and applied. In this essay, the author reviewed and appraised all kinds of HLA genotyping tech niques and intr...

本文对目前各种HLA基因分型方法的原理和优缺点结合自己的应用经验做了评价，并简要介绍了在HLA基因分型研究中出现的新的动态。

Objective To investigate the relationship between HBV intrauterine infection and gene mutation by analysis of HBV preS/S gene sequence.

目的 研究宫内传播中乙型肝炎病毒前S/S基因的突变，探讨宫内感染乙型肝炎病毒与HBV基因突变的关系。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。