基因型

Methods: We used a 5075 gene cDNA microarray to examine gene expression in peripheral blood mononuclear cells from two type 2 diabetes patients with distal symmetric polyneuropathy, two type 2 diabetes patients without distal symmetric polyneuro...

采用包含5075个功能已知人类基因的cDNA芯片检测2名2型糖尿病并发远端对称性多神经病变患者，2名2型糖尿病不伴远端对称性多神经病变患者以及2名年龄和性别匹配的正常个体外周血单个核细胞基因表达谱。

This represents the first demonstration of a genetic linkage between the pretibial phenotype and COL7A1 gene and supports that the Pasini, Cockayne-Touraine, and now the pretibial clinical variants of DEB are allelic.URI: http://grbsearch.stpi.org.tw/GRB/reportDetail.jsp?id=RB8

这是第一个显示胫前型EB与COL7A1基因有连锁的报告，进一步支持了显性DEB的三种主要的亚型，Pasini， Cockyane-Touraine及胫前型，都同样是COL7A1基因突变的表现。

The male sterility resulted from nucleus-cytoplasmic interaction (nuclear genome of common wheat--cytoplasmic genome of D〓-type cytoplasm donor), D〓-type CMS was sporophytic sterility pattern.

一、D〓型CMS系的花粉育性对光周期的变化不敏感（不同于已有光敏不育性的报道），属非光敏细胞质雄性不育系；其不育性起因于核质互作（普通小麦核基因组--D〓型细胞质变异基因组）、属孢子体不育类型；雄性不育受核内隐性基因控制、育性恢复受核内显性基因控制。

The average sequence divergence between individuals was 0.0006, while the range within a population and between populations were both 0-0.0013. Fst values were 0.0704, 0.0491, and 0.0792 for Cox1, ND1, and the combination of both genes. These data showed significant genetic variances (P.05) among geographic populations. The taimen populations in Heilongjiang River could be divided into four subpopulations based on Fst values between these geographic populations. However, these populations shared one common haplotype (BH11) shown by haplotype network and haplotype distribution frequency analysis. This suggested that they evolved from a common ancestor (likely a population in the upstream of the Heilongjiang river) and shared common gene flows.

单倍型网络和AMOVA分析结果均表明黑龙江流域哲罗鲑存在显著的群体分化，Cox1基因、ND1基因及组合数据的群体间Fst分别为0.0704、0.0491、0.0792，均达到显著性水平（P.05），根据配对群体间Fst可以将黑龙江流域哲罗鲑群体分为黑龙江上游群体、呼玛河群体、乌苏里江群体、内蒙古伊敏河上游群体4个地理群。9个群体共享同一个单倍型（BH11），表明这些群体具有相同的演化历史，为同一个祖先群体演化而来。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

结果 EB1基因mRNA拷贝数/GAPDH基因mRNA拷贝数的均值在人工流产绒毛核型分析正常的胚胎细胞中为0.01004±0.008223，在自然流产绒毛核型分析异常的胚胎细胞中为0.1509±0.0633。

Label with LC-Red640 and LC-Red750 two are aimed at above all 112 code with 158 child detect 5 ′ carry bougie, corresponding anchor decides bougie to be labelled by fluorescence in 3 ′ end, circulate quickly from inside DNA of person gene group again PCR is distinctive the enlarge that make thing adds part of ApoE gene 265bP and with distinctive bougie cross hind, cause fluorescent resonance energy to transfer, the occurrence that passes different source cross and fault match alkaline base the fusible parameter of the type will finish feral model divide with choppy gene model.

首先以LC-Red640和LC-Red750标记两条针对112和158位编码子的检测探针5′端，相应的锚定探针在3′端被荧光标记，再从人基因组DNA中快速循环PCR特异引物扩增apoE基因265bP片段并和特异探针杂交后，引发荧光共振能量转移，通过异源杂交的出现和错配碱基的类型的熔解参数来完成野生型和突变基因分型。

The materials with Wx^1 genotype and G-type of Wx gene had higher AC and harder GC, however, the materials with Wx^2,Wx^3 genotypes and T-type of Wx gene had lower AC and sof- ter GC.

利用484／485引物检测，Wx基因呈Wx^1、Wx^2和Wx^3种多态性；而用PCR-Acc I检测，Wx基因表现为G型和T-型。

The prawn lysozymes are presumed to be the non-calcium binding family of chicken-type lysozyme because they have two conserved catalytic sites Glu~(51) and Asp~(68),as well as eight structural Cys residues,which are highly conserved among the species of chicken-type lysozymes but lack of three Asp residues at the site 101,106 and 107,which are conserved sites in calcium-binding chicken-type lysozyme.

两种沼虾溶菌酶都具有c-型溶菌酶典型的两个酶活性位点(Glu51)和(Asp68)，以及8个保守结构氨基酸残基Cys，且在101、106和107位上缺少Asp，因而推测本实验所克隆的两种沼虾溶菌酶基因属c-型溶菌酶基因的非钙结合亚型。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。