基因型

The main clime features included ataxia, hypopsia, axanthocyanopsia and retinal pigmental degeneration. Alleles from 7 to 9 repeats were seen in the other 4 healthy members. GAG repeats from 6 to 21 were found in other 126 SCA patients, 71 family members and 60 healthy controls.

该家系内表型正常的4位成员SCA7等位基因CAG重复数目为7~9，另126例临床表现为SCA的患者、71名表型正常的家系成员及60名健康对照者SCA7等位基因内CAG三核苷酸重复数为6~21。

In this exper iment, we took the advantage of RNAi technique, microinjected tinman and wingless dsRNA into the early embryos in Drosophila respectively and got these two genes' RNAi phenotypes, which were very similar to that of their mutant, showing heart tube defects or no heart precursors formation.

实验运用RNAi技术，分别将tinman和wingless的dsRNA注入果蝇的早期胚胎，得到了这两个基因的dsRNA干扰表型，与两个基因的突变体表型非常相似，都表现为果蝇心脏前体细胞不能形成或心脏管缺失。

It was identified that the nif gene for aerogenic P. agglomerans strain waslocated on plasmid while for anaerogenic strains were located on chromosomes usingKlebsiella pneumoniae nifHDK as probe.

用nifHDK为探针进行Southern杂交证明固氮成团泛菌产气型的菌株固氮基因定位在质粒上，而不产气型菌株固氮基因定位在染色体上。

Cloned one virus binding protein from Rhopalosiphum maize Yangling biotype through PCR technique, using designed specific primers based on this protein partially sequence. In nucleotide and amino acid sequenece levels, this protein was identified as GroEL protem of endosymbiotic bacterium (Buchnera sp.) of aphid.

以玉米蚜杨凌生物型为材料，设计特异性引物采用PCR的方法在国内首先克隆了一种玉米蚜体内参与传毒的共生菌groEL基因，序列测定结果表明：玉米蚜杨凌生物型共生菌groEL基因全长为1647bp。

In-between the two gene clusters, homologues of Erwinia chrysanthemi virulence regulatory genes pecS and pecM were identified and named phgS and phgM respectively.

而在第一型纤毛和第三型纤毛基因群之间，我们发现有两个基因和Erwinia chrysathemi调控毒性因子的pecS和pecM相似，分别命名为phgS和phgM。

To investigate the presence of specific adhesion activity, the mrkD adhesin gene of the established type 3 fimbriae expression system was replaced respectively by each of the fimbrial adhesins. Transmission electron microscopy and western blot analysis revealed that most of the recombinant type 3 fimbriae expressed and assembled properly.

位於纤毛末端的黏附蛋白为决定细菌黏附专一性的主要因子，为了测试黏附蛋白的活性，我们进一步以过去建立的第三型纤毛表现系统为平台，将MrkD黏附蛋白基因分别置换为kpa、kpb、kpc、kpd、kpe、kpf或kpg的黏附蛋白基因，并经西方墨点法确认这些重组第三型纤毛的结构蛋白的表现和组装正常。

PCR and Southern blot analysis confirmed stable integration of the foreign genes into the chloroplast genomes of T_0 and T_1 transgenic plants, and homoplasmy of T_1 transgenic plants.

只转化phaC2基因的叶绿体型转基因烟草基本检测不到有mel-PHAs合成，共表达phaC和phaG基因的叶绿体型转基因烟草则能合成相对较高水平的mcl-PHAs。

According to whether the patients have a history of alcohol abuse divide them into the groups of alcohol and nonalcohol.

用基因芯片技术分别对两组患者进行基因分型，并比较其分型结果。

Ms-yanshi 9^ ms-80 (6) and ms-90-110, were selected as the materials in the study on gene location of B -type cytoplasmic male sterile lines by using monisomic analysis and nullisomic -tetrasomic analysis methods.

结果表明：二角型不育系主效恢复基因只有一对，位于ChrislB染色体上，在旧染色体以外，不存在二角型不育系的主效恢复基因。

Objective: To examine changes in gene expression profiling of peripheral blood mononuclear cells in type 2 diabetes patients with distal symmetric polyneuropathy as compared to type 2 diabetes patients without distal symmetric polyneuropathy and to healthy controls.

目的：利用基因芯片技术比较2型糖尿病伴有远端对称性多神经病变的患者、2型糖尿病不伴远端对称性多神经病变的患者以及正常个体外周血单个核细胞基因表达谱的差异。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。