基因型

Its alterations of phenotype and structure have been further studied at whole, organic and cellular levels. By means of backcross between wildtype and the mutant, its genetic background has been analyzed.

本文在整体水平、组织水平和细胞水平上深入研究了该突变体的表型和结构的变化、通过突变体与野生型之间的杂交对其遗传背景进行了分析，并运用分子生物学手段对突变体相关基因进行了克隆和定位，初步探讨了该基因的表达模式和功能。

To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le phenotype cells, patient phenotype was identified by using anti-Le and anti-Le blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly.

为了分析1例溶血性输血反应产生的原因，用谱细胞和Le表型细胞鉴定患者血清中的抗体，用抗Le和抗Le血型试剂鉴定患者红细胞表型，用PCR测序方法分析LE基因（FUT3基因）全编码区序列。

It was a new mousegene in GenBank and named as SRG-L(spermatogenesis related gene expressed in the latestages of spermatogenic cells). The sequence of this cDNA has been deposited in GenBankwith the Accession No. AY352586. 2. Analyses of expression characteristics of SRG-L during mouse spermatogenesis.The results of RT-PCR and Northern Blotting indicated that no expression signal of thisgene could be observed in primitive type A spermatogonia and type B spermatogonia. Asignal first appeared in diplotene/pachytene spermatocytes, and was significantlyintensified in round spermatids and elongating spermatids, findly went down to beundetectable in mature spermatozoa.

RT-PCR和Northern Blotting分析表明：该基因在早期A型和B型精原细胞中不表达，从双线期初级精母细胞阶段开始表达并逐渐上调，在粗线期初级精母细胞、圆形精子细胞及长形精子细胞中均检测到高水平的mRNA，但在成熟精子中消失，具有明显的阶段特异性表达特征，并且组织特异性表达分析显示该基因在睾丸组织中的呈高表达。

Polymerase chain reaction is amplified and sequenced to determine the type of emm genes.

聚合酶链反应扩增并进行测序从而对emm基因进行分型确定emm基因的型别。

BACKGROUND: Studies show that forkhead transcription factor 1 (Fox01) plays an important role in insulin resistance and the pathogenesis of type 2 diabetes.

背景：研究表明叉头转录因子1是一种新的2型糖尿病易感基因，但2型糖尿病患者与健康人的叉头转录因子1基因在转录水平上差异性的研究不多。

Methods The tissues of cervical carcinoma with HPV16/18positivity was injected into the hypodermis of nude mice to make tumors and transmission,then Ad-p53gene was injected into the tumors to investigate the effect,while DDP,BLM and normal saline were injected into the tumors to as control groups.

将含HPV16/18型感染的宫颈鳞癌组织注入裸鼠皮下成瘤、传代，将以缺陷型腺病毒为载体的P53基因（Ad-P53）瘤内注射，同时用抗癌药DDP、BLM以及生理盐水作对照，观察P53基因治疗宫颈癌的有效性。

The scrA disruptant showed bld phenotype on minimal middium containing glucose; but it was same as the wild type on minimal medium containing mannitol.

该基因破坏后，导致分化阻断在长直的白色气生菌丝阶段。完整基因片段可以使该突变表型得以恢复为野生型。

All these 5 genes were specifically expressed in morula stage.

兔由母源型调控向合子型调控的过渡是在8～16细胞期开始的，在桑葚期开始表达的这些基因片段应该是兔胚胎时期特异性基因。

All these 5 genes were specifically expressed in morula stage.Maternal-to-zygotic transition of rabbit starts from 8-16 cell stage,and therefore,these genes should be embryo-specific.

兔由母源型调控向合子型调控的过渡是在8～16细胞期开始的，在桑葚期开始表达的这些基因片段应该是兔胚胎时期特异性基因。

Ten alleles sequenced had the same modular structure, starting with a repeat block of Type 3 followed by a block of Type 1 before the central block of Type 3 (313) at 5'end, and starting with a repeat block of Type 4 followed by the central repeat block of Type 3 (43) at 3'end.

另一种为新发现的变异核心序列，暂命名为7型（type 7）。10个等位基因具有相同的模块结构，在5'端表现为：3型一1型--3型（313）排列，在3'端则表现为：4型一3型（43）排列。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。