基因型

The RhoA oncogene , have been revealed in recent years, are closely related to various malignant tumours. RhoA, belonging to Rho family, is one member of the ras superfamily, whose encoding production, namely RhoA protein, pertaining to micromolecular mass GTP conjugated protein family, in GTP conjunctive active form and GDP conjunctive unreactive form. It exist in the latter form in normal cells and the switch of the two forms enables them to educe functions, paralleling"molecular switch", the diminutive G protein in activated state being able to activate the wink dromo-iter to work functions, which is in the resemblance of process during which the Ras genic mutation induces its continous activations.

RhoA是近年来新发现的与多种恶性肿瘤密切相关的癌基因，RhoA属于Rho亚家族，是ras基因超家族的一员，其编码产物RhoA蛋白属于小分子量GTP结合蛋白族，分为GTP结合的活性型和GDP结合的非活性型两类，在正常细胞内以GDP结合的非活性型存在，两种活性形式之间的转换使他们能够发挥一种类似"分子开关"的作用，活性态的小G蛋白能激活下游的信号传导通路，行使其功能。

Result The study showed that there were two isozymes of serum amylase including Amy-1 and Amy-3 in Qinghai Alectoris graeca.

结果］青海石鸡血清淀粉酶有Amy-1，Amy-3 2种同工酶，其中Amy-1有AA、AB和BB 3种表型，表型频率分别为17.95%、66.67%、15.38%；AB为优势表型，Amy-1和Amy-1等位基因频率分别为0.5128和0.4872，基因杂合度为0.4997、青海石鸡血清淀粉酶活性为(566.62±112.20)U/L。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Objective The GJB2, GJB6 and GJB3 genes are related to hereditary deafness and keratoderma. To investigate the genetic causes, we recruited a Chinese family and screened the proband with syndromic deafness and palmoplantar keratoderma for mutations in these three genes and analyzed the phenotype and hereditary traits.

目的 GJB2、GJB6、 GJB3基因与遗传性耳聋及角化病有关，以GJB2、 GJB6、 GJB3基因为候选基因，研究1例伴有掌跖角化病的综合征型耳聋先证者的分子病因，探讨其表型及遗传特征。

Results: pcDNA3.1+/TSP-1-Ⅰ veinal injection has stronger TSP-ltype Ⅰ repeat band than that of directly injection into myocardium (P＜0.05), while veinal injection of pcDNA3.1-/anti-TSP-1-Ⅰ has a slightly weak TSP-1 typeⅠ repeat band (P＜0.05), Western blot obtained the same result.

成功构建pcDNA3.1+/TSP-1-Ⅰ、pcDNA3.1-/anti-TSP-1-Ⅰ。作为血管新生抑制基因，在体外，TSP-1Ⅰ型重复序列基因编码的TSP-1Ⅰ型重复序列多肽，能够抑制ECV304细胞生长和增殖，其抑制机制与TSP-1Ⅰ型重复序列诱导ECV304细胞凋亡相关。

Further studies showed some DH plants could generate two or more than two stems in which wrinkledness mutant and wild types except for the leaf wrinkledness, therefore they were the better materials for studying the wrinkledness characteristic. The finding is useful in studying, locating and cloning the wrinkledness gene linked to leaf mutants of Brassica allosextuple CGMCC № 2553 DH lines in the future.

进一步研究发现，在同一DH再生植株上常常会萌发出皱缩和野生型的2个或多个分枝，由于皱缩和野生型除了叶片皱缩性状不同外，其他性状完全相同，因此叶片突变体和野生型是研究叶片皱缩性状的理想材料，有利于进一步研究皱缩基因的遗传，定位、克隆叶片皱缩相关基因。

In this study, genetic analyses were conducted to determine the genetic basis in an elite resistant inbred line Siyi with complete resistance to maize dwarf mosaic. A new genetic model, two dominant complementary genes conditioning the resistance, were found by Mendelian genetic analysis based on parents, F1, F2 and backcrosses in three successive years' field trails. The two genes were further mapped near the centromere of chromosome 3 and 6, respectively by tightly linked microsatellite markers using 242 plants from F2 generation. The resistance gene on chromosome 3 is 1.0 cM apart from the flanking markers phi053 and umc1527, respectively. Whereas the linkage distance between two flanking markers bnlg1600 and phi075 and resistance gene on chromosome 6 was 1.0 and 4.0 cM, respectively. Genotypic analysis of the plants from testcross and F3 populations supported the new genetic manners.

课题组通过连续三年的抗病鉴定，在国内种质资源中筛选出一份综合农艺性状优良、配合力较高的自交系四一，三年的表型遗传研究和两年的分子标记工作，发现四一中的玉米矮花叶病抗性是由两对显性互补基因控制的，进而利用F2作图群体，把发现的两个基因定位在第三和第六染色体的着丝点附近，并获得了双侧紧密连锁的分子标记连锁图谱，其中第三染色体上的分子标记UMC1527和phi053从抗病基因双侧逼近1 cM，而第六染色体上的分子标记phi075、bnlg1600从抗病基因双侧分别逼近4 cM和1 cM；利用B2群体、F2：3家系、BC3F1群体和带有第三、第六染色体抗病基因以及两个抗病基因的近等基因系，进一步证实了四一中成株期抗性是由两个显性互补基因控制的。

Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea, smaller lemma/palea, and stunted lemma/palea was compared by Real Time RT-PCR.

从刘(2003)所建立水稻颖花发育相关候选基因库中，挑选6个基因：salT基因(salt-induced protein)、GA-SPY基因(gibberellin action negative regulator SPY-related protein)、U2AF基因(U2 snRNP auxiliary factor，small subunit-related protein)、kinesin-like基因、DnaJ-like基因、及EF hand基因，以水稻正常型颖花、小颖花突变体及内外颖退化突变体之叶片及1~4公分幼花序为材料，利用Real-Time RT-PCR分析，发现salT基因在突变体幼花序中大量表现。

There is not a significant difference in these groups by one-way analysis of variance. 2 Among 51 cases with lung cancer, 7 specimens showed malignant cells in bronchial lavages, including 4 cases in central lung cancer and 3 cases in peripheral lung cancer. Concordant results were observed in 20 cases. 6 specimens showed malignant cells on cytological analysis, and NMSP was positive in at least one gene tested. 14 samples did not show malignant cells on cytological analysis, and the NMSP results were correspondingly negative. The results of cytology and NMSP were discordant in 31 samples. 30 samples were cytologically negative, but NMSP positive in one or more genes. In addition, there was one case that the cytology was positive for malignant cells, but the NMSP was negative in all of the three gene tested.

51例肺癌患者支气管灌洗液细胞学检查发现肺癌细胞7例，其中27例中心型肺癌患者4例支气管灌洗液发现肺癌细胞，24例周围型肺癌患者3例支气管灌洗液发现肺癌细胞。20例支气管灌洗液细胞学检查和过甲基化检测得到一致结论。6例支气管灌洗液发现肿瘤细胞，过甲基化分析至少一个基因阳性。14例灌洗液未发现肿瘤细胞，三个基因过甲基化分析均为阴性。31例支气管灌洗液细胞学检查和过甲基化检测得到不一致结论，30例细胞学检查为阴性，但过甲基化分析一个或一个以上基因为阳性。1例细胞学检查阳性但过甲基化分析阴性。

Mitochondrial cytochrome b sequence were used to study the molecular evolution and phylogeny of Anser cygnoides(15 breed of Chinese goose), Anser anser(2 breed of domestic European goose) and Anser albifrons(the lesser white-fronted goose, Genbank accession number AF363031). Sequence analysis revealed that there were 29 variable sites and 4 haplotypes in 45 sequences, nucleotide diversity and haplotype diversity were 0.0068,0.45 respectively, insertion/deletion or frameshift mutation were not found in Cyt-b gene sequences(1143bp).

本实验测定了中国家鹅15个品种、欧洲鹅2个品种共44个个体的细胞色素b基因全序列，与Genbank库中自额雁细胞色素b基因序列合并在一起比对分析，17个家鹅品种和白额雁细胞色素b基因全序列均为1143bp，序列中没有缺失、插入或移码突变，共检测到29个变异位点、4种单倍型，核苷酸多样度和单倍型多样度分别为0.00648和0.45。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。