基因型

The two isolates were more closely related to CTN, with the homogeny of N gene and G gene as 89.1% and 85.6%-85.7% at the nucleotide level, but 97.6%-98.0% and 92.3% at the amino acid level respectively, than to other vaccine strains.

结论2株狂犬病毒是基因1型狂犬病毒，但无论是N基因还是G基因的核苷酸序列，以及推导出来的氨基酸序列与已知的1型狂犬病毒株及疫苗株均有一定的差异。

In the second part, in order to compare the effect of function of different SNPs, we use functional assay to detect the LUM under the condition of site-directed mutation. Moreover, since MAP kinase pathway is important in survival and apoptosis of cells, we use luciferase reporter assay to detect the influences of the transcription of LUM in mitogen-activated protein kinase pathway.

本研究的第二部分在探讨这些基因多型性的功能，这是以表现型功能性质体的作用，藉由各种不同基因多型性在其中的表现，来比较这些不同的基因变异是否会有功能上的改变，及比较其造成改变的强弱。

KCR and HCD are erucic acid synthesis genes. In order to reveal the relationship between the synthesis of erusic acid and the quantitative expression of its functional genes, and to reveal the theoretic base of the breeding for high erucic acid content, the expression of KCR and HCD had been analyzed kinetically and quantitatively by using regular reverse transcription polymerase chain reaction and the internal standards of QuantumRNA18S during the growing period of high erucic acid content lines of Brassica Juncea.

研究应用常规RT-PCR(Reverse transcription polymerase chain reaction)的技术和QuantumRNA18S内标对芥菜型高芥酸油菜生育期内芥酸合成基因KCR、HCD基因的表达进行量化分析，了解芥酸的合成与功能基因量化表达的关系，为芥菜型油菜的高芥酸品种的选育提供理论依据。

The present study was designed to elucidate the role of apoE polymorphism in the lithogenesis of cholecystolithiasis and to explore the hereditary pathogenesis of the disease. Polymerase Chain Reaction was used as researching apoE phenotypes and allele frequencies in patients with gallstones (n=87) and in controls (n=50), and the fasting serum lipids of subjects were also measured.

为从分子遗传学水平探讨胆囊结石病的发病机理，采用聚合酶链反应等方法研究了87例胆囊结石患者和50例非结石者的apoE基因表型及等位基因频率，并分析了不同apoE基因表型的胆囊结石患者的血脂质代谢特征。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya，以绿色荧光蛋白基因为报告基因，构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46，荧光显微镜检测发现，此启动子在两菌株中都具有功能。

Then the fusion protein was primary purified by affinity chromatography. The GST tail of the fusion protein was cleaved by Thrombin. Nucleotide sequence analysis showed that the gdh gene of S.suis possesses the highly conserved motifs typical of family I hexameric GDHs.

猪链球菌2型谷氨酸脱氢酶编码基因克隆、序列分析及原核表达根据已发表的猪链球菌2型的gdh基因设计引物，扩增海安病人分离株Habb中的目的基因，并进行序列测定和分析；构建重组表达载体pGEX4T-2-gdh，在大肠杆菌中表达，并纯化重组蛋白。

To inquire into association between susceptibility to AIDP, AMAN and frequencies of HLA alleles and possible differencies in distribution of HLA alleles betwen AIDP and AMAN by genomic typing for HLA-class Ⅰ, class Ⅱ alleles, and to try to found out the effects of self intrinsic fators of GBS patients on occurence of two subtype from immunogenetic angle.

通过对AIDP和AMAN两种GBS亚型的HLA-Ⅰ类和Ⅱ类等位基因的分型，探讨两种亚型的易感性与HLA等位基因分型的关系以及两种亚型在HLA等位基因分布上可能存在的差异，从免疫遗传学角度寻找自身内在因素在GBS不同亚型发病中的可能作用。

The results suggest that the average level of serum lipids in the same apoE phenotype patients with gallstones is higher than that in controls, and the different apoE phenotypes patients with gallstones have different characteristics of dyslipidemia.ε2 allele is probably one of the dangerous factor in the lithogenesis of cholecystolithiasis.

由此表明：同一apoE基因表型胆囊结石患者的血脂质变化比非结石者突出，不同apoE基因表型的胆囊结石患者有不同的血脂变化特征，ε2等位基因可能是国人胆囊结石病的一个高危易患因子。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

Sequence analysis and homology alignment showed that MD1 had 93% similarity with matK in maize chloroplast genome, a gene encoding maturase included in type Ⅱ intron splicing of RNA transcript; MD2 had 99% similarity with gene PP2C, encoding serine / threonine protein phosphatase type 2C in extremely drought tolerant Sporobolus stapfianus; and MD3 had 99% similarity with rice gene encoding metacaspase, belonging to aspartic acid specific cysteine caspases.

序列分析和同源性比对表明，MD1与玉米叶绿体基因组中编码参与RNA转录本Ⅱ型内含子剪切的成熟酶的matK基因有93%的相似性，MD2与极端耐旱的Sporobolus stapfianus的丝氨酸／苏氨酸2C型蛋白磷酸酶基因PP2C的相似性达99%，MD3与属天冬氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase基因有99%的相似性。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。