基因型

In this study,we have identified C2H2 ZFP genes in the silkworm,and acquired the basic information for these genes,such as gene distribution on chromosome,gene family information and expression information.This information will be useful for further functional studies on these genes.

综上所述，本研究对家蚕基因组中的C2H2型锌指蛋白基因进行了鉴定，并通过分析获得了这些基因的基本信息，即染色体分布、基因家族信息和基因表达信息等，这些信息为进一步对家蚕C2H2型锌指蛋白基因进行功能研究打下了基础。

The lack of liver and kidney type PBMC in patients with alopecia areata Thl/Th2 type cytokine gene expression imbalance, presented to Th1-type cytokines and transcription factor T-bet gene over-expression of Th1-type response characteristics, may be involved in the pathogenesis of alopecia areata, alopecia areata may be insufficient evidence of liver and kidney-shaped one of the micro-nature.

肝肾不足型斑秃患者PBMC中Thl/Th2型细胞因子基因表达失衡，呈现以Th1型细胞因子和转录因子T-bet基因过度表达为特征的Th1型反应，可能参与了斑秃的发病机制，也可能是斑秃肝肾不足证型的微观本质之一。

In this exper iment,we took the advantage of RNAi technique,microinjected tinman and singless dsRNA into the early embryos in Drosophila respectively and got these two genes' RNAi phenotypes,which were very similar to that of their mutant,showing heart tube defects or no heart precursors formation.tinman dsRNA eve caused visceral mesoderm defects and the somatic muscles disruption,yet wingless dsRNA only affected heart precursors and had no effect on visceral mesoderm and somatic muscles,indicationg that the heart-related genes dsRNA interference worked effectively and exclusively in Drosophila.

实验运用RNAi技术，分别将tinman和wingless的dsRNA注入果蝇的早期胚胎，得到了这两个基因的dsRNA干扰表型，与两个基因的突变体表型非常相似，都表现为果蝇心脏前体细胞不能形成或心脏管缺失。尤其是tinman基因的dsRNA，还引起了肠中胚层缺失和体壁肌肉组织的紊乱，而wingless基因的dsRNA却只影响心脏的形成，而不影响肠中胚层，说明dsRNA干扰具有非常强的特异性，因而不失为研究果蝇心脏发育基因功能的有效方法。

To date, one allele coding fast acetyltor (wild-type NAT2*4) and several mutated alleles coding for impaired acetylator activity have been identified.

NAT2基因具有高度的多态性，至今为止1个快型野生型等位基因(NAT2*4)和一些慢型野生型等位基因已被证实。

As to Z1, longjiao 91060-2 and Balilla, the curature of panicle neck is significantly positively correlated with height, and the panicle type gene may link with height.

对Z1、龙交91060—2和巴利拉型直立穗型品种来说，颈穗弯曲度与株高呈显著或极显著的正相关，但穗型基因与株高基因可能是连锁关系而不是同一基因。

The genetic property of Balilla type is controlled by a pair of dominant nuclear genes, and modified by minor genes. Contrast with curved panicle gene, the erect one is dominant, but may not the complete dominant.

巴利拉型直立穗型品种的穗型遗传受1对主效核基因控制，同时有微效修饰基因参与作用，直立穗型相对于弯曲穗型为显性，但不一定是完全显性。

Results: ApoD was observed positively expressed in type2 astrocytes and oligodendrocytes from O2A lineage, while it was not expressed in type1 astrocytes from T1A lineage. Accordingly, we can verify the phenomenon found in the study of T1A and T2A gene different expression profile that ApoD mRNA was highly expressed in T2A and lowly expressed in T1A. Conclusion: It can be concluded that lipid metabolism related genes such as ApoD play important roles in the generation and differentiation of O2A lineage, which closely correlate with internal mechanism of lipid metabolism in the brain and myelogenesis.［Key words］astrocyte(T1A, T2A); oligodendrocyte; O2A lineage; apolipoproteinApoD

我们已采用基因芯片技术在mRNA水平研究两型星形胶质细胞的基因表达谱差异，发现ApoD在2型星形胶质细胞中差异高表达，故本文拟用激光共聚焦双重免疫荧光标记技术进一步在蛋白质水平观察ApoD在体外分化成熟的两型星形胶质细胞以及少突胶质细胞中的表达情况，以期为我们深入研究ApoD等脂代谢相关基因在脑内脂质代谢和神经髓鞘发育机制中所起的作用提供有用的实验依据，依此探讨胶质细胞发生和分化的信号机制。

To prepare recombinant fox growth hormone, we amplified its cDNA from silver fox pituitary tissue by RT-PCR and cloned into yeast shuttle vector pPIC9K down stream of α-factor signal peptide sequence by SnaB I and Not I restriction sites. The recombinant secretion vector pPIC9K/fGH, linearized by Sal I, was transformed into histidine-deficient Pichia pastoris strain GS115 by electroporation.

为制备重组狐狸生长激素，采用RT-PCR方法，从银狐垂体中扩增fGH cDNA基因，利用SnaB I和Not I位点将fGH基因插入到酵母分泌型表达载体pPIC9K中α-因子信号肽的下游，构建成fGH基因的酵母分泌型表达载体pPIC9K/fGH，载体经Sal I酶切线性化后，通过电转移将线性化的pPIC9K/fGH转化到组氨酸缺陷型酵母宿主菌GS115中。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

K-means, and self-organizing map and the feature selection methods based on coefficient of variation and simple T-test were integrated. To evaluate the performance of the Samcluster system, the Samcluster was applied to four expression datasets COLON, LEUKEMIA72. LEUKEMIA38, and OVARIAN. The results show that there are only 5, 1,0, and 0 samples misclassified, respectively. We conclude that the proposed scheme. SamCluster. is an efficient method for automatic discovery of sample classes using gene expression profile.

在Samcluster系统中，整合了下列聚类算法：谱系聚类、K-平均值聚类和自组图聚类与变异系数计算和T-检验等基因变量选择方法，并提出了一致的样本分型概念，通过对四个基因表达谱的数据集COLON、LEUKEMIA72、LEUKEMIA38和OVARIAN的测试，结果表明：误判的样本数分别为5、1、0和0个，因此，基因水平的样本分型与样本的临床分型高度一致。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。