基因型

After analyzing glutinous rice waxy gene sequence reported by previous studies, two pairs of dominant and codominant STS molecular markers based PCR were designed for distinguishing glutinous rice, according to the distinctions between glutinous rice wx gene and nonglutinous rice Wx gene. Moreover, the whole sequences of the two Wx genes of nonglutinous rice reported by others were also aligned, and another two pairs of dominant STS molecular markers were designed to discriminate Wxa from Wxb based on the special site between the two genotypes. Then, corresponding rice genotypes were identified by the established new markers.

通过对前人公布的糯性水稻蜡质基因全序列测定结果的分析，根据糯性水稻与非糯性水稻蜡质基因序列位点的差异，设计了两个基于PCR扩增反应的可特异识别糯性水稻的显性和共显性STS分子标记；同时在前人公布的非糯性水稻蜡质基因的不同类型的全序列比对分析的基础上，选取了两种基因型特定的差异位点，设计了两个基于PCR扩增反应用于特异识别这两种Wx基因的显性STS分子标记，并用新建立的标记对相应的水稻基因型进行了检测。

The results of all ABO genotyping in 50 families observe the Mendel's Law and were fully consistent with their ABO phenotypes.

同一个体的ABO基因型与ABO表型结果完全相符。100例认定嫌疑人的强奸案中混合斑精子的ABO基因型与相应嫌疑人血样的ABO基因型结果完全一致。

The full F gene was sequenced and compared with the published sequences of virulent and avirulent strains of NDV in GenBank database.

根据分离株序列与GenBank中NDV相关毒株序列比较分析，结果表明，所分离的6个分离株归属为3个NDV基因型，其中有基因Ⅶd亚型（3株）、基因Ⅵ型（2株）和基因Ⅲ型（1株）。

1Livestock Research Institute, Council of Agriculture(2)Department of Animal Science, National Pingtung University of Science and TechnologyGenetic variation in the N-acetylglucosamine 6-sulfatase (G6S) gene is the key role in caprine Mucopolysaccharidosis IIID.

本研究建立山羊黏多醣症遗传缺陷之单股构型多态性基因型检测方法，应用此方法来检测乙醯醣胺氨基硫酸酶(N-acetylglucosamine 6-sulfatase， G6S)基因型不再需要使用限制酶，可节省检测时间、成本与人力。G6S基因的遗传变异在山羊黏多醣症第三型扮演重要的角色。

Moreover, in comparison of birth lengths in eight subgroups defin ed by organic solvent status (nonexposure/exposure) and maternal genotype for MS P1 and GSTM1, in the presence of organic solvents ' exposure , the birt h length was a clear gradient of adverse effects .

3我们将芳香烃溶剂，母亲基因MSP1（野生型、杂合子/突变型）与基因GSTM1分为8组，在芳香烃溶剂暴露组，可见母亲基因型致出生身长呈斜线降低，然而在芳香烃溶剂非暴露组，其母亲基因型对出生身长的影响无明显改变。

Results:(1) totally, the distributions of alleles o r genotypes of drd3 ser9gly polymorphism are not significantly different between the groups of paren ts, unaffected-siblings and affected-siblings, and between the sub-types of schi zophrenia.

对drd3基因的ser9gly多态性进行检测。结果：(1)总体而言，drd3基因的ser9gly等位基因频度和基因型频度在父母组、非患病同胞组和患病同胞组之间差异无显著性，在精神分裂症不同亚型之间基因型频度和等位基因频度的分布差异也没有统计学意义。

Methods The method of PCR- RFLP was conducted to examine the genotype of 3 cPLA2 genes ( PLA2G4A, PLA2G4B and PLA2G4C genes) in 263 subjects, including 121 cases of T2DM and 142 controls. The conditional test was used to test the combined effect of distinct loci on the T2DM by conditioning on allele or by conditioning on genotype with UNPHASED analysis platform. Results The genotypic frequencies of the 3 genes did not deviate from Hardy-weinberg equilibrium in both case and control groups.

采用聚合酶链反应-限制性内切酶片段长度多态性方法检测T2DM患者（病例组121例）和健康对照（对照组142例）3个cPLA2基因（PLA2G4A、PLA2G4B和PLA2G4C基因）的基因型；借助UNPHASED分析平台，利用等位基因条件分析和基因型条件分析方法分析基因的联合作用与T2DM的遗传相关性。

Methods Polymerise chain reaction and restriction fragment length polymorphism were used to analyze the polymorphisms of MTHFR genes C6677T and A1298C in 56 patients of colorectal cancer and 143 age-and-sex-matched control subjects.

方法以聚合酶链反应和限制性片段长度多态方法，对56例直肠癌患者和143例正常人对照的MTHFR基因C677T和A1298C基因型进行检测分析其对直肠癌风险的相关性。结果在正常对照组中，MTHFRC677TCC、CT、TT基因型频率分别为62·90%、35·08%和2·12%，而在直肠癌病人中分别为58·93%、26·77%和14·26%。A1298C与A1298C基因型在病例和对照中的分布差异无显著性意义。

Methods The genotype of 4 SNPs in 108 simple CHD patients and 200 normal people were analyzed by restriction fragment length polymorphism and denaturing high-performance liquid chromatography. The statistical contingency table method was used to analyze SNP genotype frequency and gene frequency in patients and control group;then, the haplotypes were established and their frequencies in the two groups were assessed by PHASE software.

方法应用限制性片段长度多态性和变性高效液相色谱法结合测序，分析108例单纯性先天性心脏病患者及200名正常人4个SNP位点基因型；应用列联表法统计分析患者组和对照组各SNP位点基因型及等位基因频率；应用PHASE软件构建单倍型并统计分析患者组及对照组单倍型频率是否存在差异。

As PTDT does in threshold traits, PTDT is valid not only for different QTL effect level, but also for maker with multiple alleles and multiple tightly linked markers.(2) Under an appropriate selection ratio s (in this study, s is 0.2, 0.4, 0.6, 0.8, respectively), the power of PTDT can be improved and the genotying individuals can be decreased using selective genotyping design. However, the power of PTDT is related with population size and population structure, an appropriate selection ratio can be defined by simulation based on the existing data.(3) Among the three transforming methods, mixed-family selection is the best, full-sib selection has same power to mixed-family selection in many parameter combinations, and Estimated Breeding Value selection is inferior to them.

数量性状QTL定位的模拟研究结果表明：(1)数量性状经有效转化后，PTDT对数量性状QTL定位保持了阈性状QTL定位的稳健与高效，对不同效应大小的QTL(10％，30％，50％)PTDT都是一个有效的分析方法；(2)在多等位基因标记、多标记方面，PTDT的检验功效与阐性状分析时一样高效；(3)在合适的选择率下(本研究的分别为0.2，0.4，0.6，0.8，1.0)，选择性基因型测定不仅可以减少基因型测定的数量，而且可以提高PTDT的检验功效，但选择性基因型测定的PTDT检验功效很大程度上与群体大小和结构有关，这个可根据具体情况通过模拟找到一个合适的选择率；(4)三种转化方法的转化效力结果表明，家系内混合家系选择的转化效力最高，家系内全同胞选择次之，估计育种值选择的转化效力较差。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。