基因型

Analyzed the correlation of different genotypes and productive performances in Sutai pigs with the BB、BD and DD genotypes of HaeⅢdigestion analysis and Sutai pigs with the AA、AB、BB genotypes of RsaⅠdigestion analysis, the results showed significant correlation with postweaning viability in the second litter, and the significant correlation with the total litter, number of viable pigs, litter weight at birth, number of ablactation pigs and litter weight after ablactation in the third litter.

8苏太猪SLA-DQB不同基因型的生产性能比较表明，HaeⅢ酶切的3种基因型BB、BD和DD分别与RsaⅠ酶切的3种基因型AA、AB、BB相对应，HaeⅢ酶切的3种基因型和RsaⅠ酶切的3种基因型均与2胎断奶成活率显著相关，与3胎总产仔数、产活仔数、初生窝重、断奶仔猪数和断奶窝重显著相关，与6胎总产仔数、断奶仔猪数和断奶窝重也显著相关。

The result showed that the best time for sampling was 7 to 14 days after the first bud flowering on the main anthotaxy. The capacity of embryoid production varied significantly in different genotypes. Among them, twelve genotypes yield embryos successfully, which the rate could reach 71%. Genotype 102 was an ideal material for embryoid induction, and the frequency could reach 32.78 embryos per bud. Genotype that with a highest yield produced 546 times as many embryos as that with a lowest yield. The embryoid induction ability of normal bolting genotypes were higher than easy bolting and hard bolting genotypes.

结果表明：在秋冬季日光温室内，供试植株主花序第一朵花开后第7~14天取样，即初花期是最佳取样时期；不同基因型间小孢子胚胎发生能力差异很大，在接种的17个基因型中，有12个诱导出胚，诱导成功率71%；其中102号产胚量最高，达到32.78个／蕾；在已产胚材料中，产胚量最高的基因型是产胚量最低的基因型的546倍；较易抽薹型材料的产胚能力大于易抽薹型和不易抽薹型。

In Small Tail Han sheep, Chinese Merino sheep, Corriedale sheep and South African Mutton Merino sheep, frequency of AA genotype was 0.58, 0.98, 0.96, 1.00, frequency of AB genotype was 0.40, 0.02, 0.04, 0.00, frequency of BB genotype was 0.02, 0.00, 0.00, 0.00, respectively. The ewes with mutation homozygous genotype BB or mutation heterozygous genotype AB had 1.45 at 305 bp of exon 3 of BMP4 gene in the BB genotype in comparison to the AA genotype, and this mutation resulted in an amino acid change: alanine—?aspartic acid. The ewes with genotype BB had 0.61 P

结果表明：小尾寒羊、中国美利奴绵羊、考力代绵羊在INHA基因5'调控区发生了1处碱基突变(316C→T)，南非肉用美利奴绵羊没有发生这种突变；小尾寒羊、中国美利奴绵羊、考力代绵羊、南非肉用美利奴绵羊AA基因型频率分别为0.58、0.98、0.96、1.00，AB基因型频率分别为0.40、0.02、0.04、0.00，BB基因型频率分别为0.02、0.00、0.00、0.00；突变纯合型和突变杂合型小尾寒羊平均产羔数分别比野生型多1.45只P

This established reverse genetics system provides a powerful tool for the research of the P gene in this study.1.Full-length genome analysis of Newcastle diseases virus strains belonging to genotypeⅢandⅨreveals the common origin of recent epizootic viruses.

基因Ⅲ型和Ⅳ型新城疫病毒全基因组测序及其在新城疫进化史中的重要地位分析根据EMBL/GenBank中已发表的基因Ⅲ型NDV全基因和基因Ⅸ型NDV基因片段设计一套全基因引物，将NDV全基因分为10个相互重叠的片段进行RT-PCR扩增。

The result implied that genotype BG of MCW0294 and genotype JL of MCW0258 may be regarded as candidate locus for early assistant selection markers in egg shape index and haugh unit traits of silkies.At the same time, we found some alleles associated with egg quality traits:(1) Means of shell strength for genotype AG and BG of MCW0258 were significantly higher than those of HO and JO.

在多重比较过程中，发现了几个与蛋品质性状显著相关的等位基因：①标记MCWO258中，AG基因型的蛋壳强度均值最高，HO基因型的蛋壳强度均值最低，二者差异显著；BG基因型的蛋壳强度均值较高，JO基因型的蛋壳强度均值较低，二者差异显著。

There was no significant difference in larval survival, sex ratio, pupation and adult emergence among four genotypes.

结果表明，抗性基因型在产卵量、卵孵化率、幼虫及蛹历期和成虫存活期等方面与敏感基因型相比较存在明显的不利性；与敏感基因型相比，抗性基因型只有045的适合度，RS和SR基因型的相对适合度分别为114和108。

Being stratified by gender factors, the CRC risk in females with homozygous W/W or Mx/W+W/W fast acetylators increased 2.47- and 2.13-fold compared to those with the Mx/Mx slow acetylator genotype (95% CI 1.27-4.82 for W/W genotype; 95% CI 1.17-3.89 for Mx/W+W/W genotype), however, the risk of the NAT2 genotype and CRC was not observed in males.

但在 W/Mx NAT2慢速代谢基因型与Mx/W+W/W快代谢基因型则无差异。若将性别分开分析时发现，女性具有NAT2 W/W homozygous 或 Mx/W+W/W快速代谢基因型发生大肠直肠癌的危险性是Mx/Mx慢速代谢基因型的2.47倍及2.13倍，但在男性则没有观察到 NAT2 基因型与大肠直肠癌的相关性。

In our study, 3 genotypies have been respectively demonstrated in UL139 and UL149 for the first time, and the corelations between certain genotypical structure and certain diseases were also proposed; The existence of the 3 genotypes of UL144 was foremost verified in isolates from congenital infants; Furthermore, the UL140, UL141 and UL145 genes were observed to be greatly discrepant to those had been described previously: comparing with Toledo, 231 nt are inserted in UL140, 2 more ORFs are obtained in UL141, and the UL145 ORF moves upstream by 90 nt. Except UL144, the sequences of 18 else genes in clinical isolates were submitted for the first time, and 479 sequences were assigned by GeneBank in total. Relevantly, 9 papers were published.

我们在学术界首次证实了HCMV UL139、UL149两个基因在临床分离株中分别存在三种基因型，发现了其中某个基因型的基因结构与特定来源的分离株存在一定的对应关系；首次在先天感染分离株中验证了UL144三种基因型的存在；首次证实了UL140、UL141和UL145等基因与原先认识的基因结构不同，其中UL140基因较Toledo株增加了231个碱基、UL141产生2个新的基因编码区、UL145 ORF较Toledo株前移90个碱基；首次或最先提交了除UL144基因外其余18个基因的临床分离株序列，本项目组共有479个HCMV相关基因序列被GenBank收录；发表研究论文9篇。

The HPV positivity amongst the group of women with no cervical abnormality was 43%, while that in women with cervical dysplasia and cervical cancer was 81.2% PCR products from 89 HPV positive cases were genotyped by LiPA, of which 49 cases were single HPV infected concerning 11 different HPV types and the commonest type was HPV-16, occurring in 25(51%) of all single HPV infected cases. The other 40 specimens contained multiple HPV types comprising 16 different HPV types. Although double infections(n=25) prevailed, triple(n=9),quadruple(n=3) and quintuple(n=3) infections were also found. PCR products of four HPV positive cases failed to be classified by LiPA. Sequencing analysis revealed that these 4 cases were HPV-66 which was not included in LiPA probes. Follow-up data of 12 cases confirmed the HPV genotyping results. Conclusion The LiPA for HPV genotyping is easy to perform.

结果 155例妇女子宫颈刮片中HPV DNA检出率为60%，其中在宫颈细胞学检查正常或仅有炎症病变的妇女中HPV DNA阳性率为43%，而在宫颈细胞增生不良或宫颈细胞癌的妇女其阳性率为81.2%。93例HPV阳性的标本中，89例利用LiPA成功地作出了基因分型，其中单独一型HPV感染为49例，涉及11种不同基因型HPV，最常见的型别为HPV 16，占单独感染的51%(25/49)；另40例为不同型别HPV重叠感染，涉及所有16种HPV基因型别，二重感染占主导地位(n=25)，同时也检测出其它多重感染。4例HPV阳性的标本应用LiPA未能作出基因分型，DNA序列分析显示这4例为HPV 66感染，此型别不包括在LiPA所标记的探针内。12例随诊资料显示了LiPA的物异性及高度的重复性。

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状，即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况，本研究从筛选玉米转基因工程所需要的优良受体入手，采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法，以及现代分子标记定位主效QTL分析方法，对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究，取得以下主要研究结果：(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合，在相同培养条件下，对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定，发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数，但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系，有的基因型，如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异；有的基因型，如自交系综31，仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599，但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距，仅为19-599和18-599的1/3左右；有的基因型，如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低，平均仅在3％左右，而且胚性愈伤组织绿苗发生数表现也很差，基本上没有分化成苗。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。