基因型

Aims: To evaluate the effects of IL-1β-511 allelomorph and it's genotype in the development of gastric cancer, and to further reveal the relationship between IL-1β genetic polymorphism and gastric cancer.

目的：综合评价IL-lβ-511等位基因及其基因型在胃癌发生中的作用，以期较深入地揭示IL-lβ基因多态性与胃癌的关系。

Methods Sequence specific primers-polymerase chain reaction was used for the detection of genotypes and alleles of rs3850641 A/G loci in OX40L gene in 187 PACS cases (≤55 years) and 138 controls. Diseased vessel numbers and stenostic severity integral by coronary arteriogram and serum levels of sOX40L, C-reactive protein and vascular cell adhesion molecule-1 (VCAM-1) by enzyme-linked immunosorbent assay and nephelometry were measured. The relationship between OX4OL gene variants and PACS risk, SSI as well as plasma levels of three cytokines were analysed.

采用聚合酶链反应－序列特异性扩增技术检测187例年龄≤55岁、早发急性冠状动脉综合征患者和138例冠状动脉造影正常者rs3850641位点A/G变异的基因型、等位基因及其分布频率；采用冠状动脉造影测量病变血管支数和狭窄程度积分；应用ELISA法测试血浆sOX40L、血管细胞黏附分子(VCAM-1)；应用散射比浊法测试血浆C反应蛋白水平；分析OX40L基因变异与ACS风险、冠状动脉病变程度和血浆3个细胞因子之间的关系。

This study adopts PCR method of coagulase - and sequencing RouGe detection a fragment of gene ESR polymorphism, and its single-nucleotide polymorphisms egg and correlation analysis, explore properties with different genotypes RouGe egg performance, from the relationship between genetic level RouGe reveal candidate function mechanism of molecular genetics of egg performance, RouGe new strains of selection for the use of genetic material.

本研究采用PCR-SSCP和测序的方法检测肉鸽ESR基因某一片段的多态性，并将其单核苷酸多态性与产蛋性能进行相关性分析，探索不同基因型与肉鸽产蛋性能之间的关系，旨在从候选功能基因层次揭示肉鸽产蛋性能分子遗传机理，为肉鸽新品系的选育提供可借鉴的遗传资料。

In this study we mapped a grain length controlling gene named Lk-4 with SSR and CAPs markers by screening 800 recessive plants in a BC2F2 population derived from a cross of Shuhui527×Xiaoli and backcrossed with Xiaoli as the donor parent.

对F2和BC2F2 群体籽粒大小形状和千粒重的遗传分析表明，回交能将大部分对目的基因效应具有干扰修饰作用的微效基因多态性除去，从而有利于对目的基因型的准确鉴定；在F2和BC2F2群体中只发现两类籽粒长短表现型，即短粒和长粒，并且二者分离比例符合3：1的典型一对等位基因分离比例。

Polymorphisms in ESRa gene and association with egg production traits The SSCP detection of 5" flanking regulation region and intronl in ESRa gene showed that two genotypes were detected in 5"flanking regulation region of blue eggshell chicken.

ESRa基因多态性与产蛋性状关系 ESRa基因5侧翼调控区和内含子1区经PCR-SSCP检测，发现绿壳蛋鸡5例翼调控区有两种基因型，两等位基因C的频率为0.86，D的频率为0.14。

This paper pointed out that the essential of geoherbs is continuers quantities variation at population level, geoherb's populations are different in gene frequency with the others'; geohersm are quantitative trait loci controlled by multi-gene or combination with multiple-gene and major gene at individual level.

指出道地性的遗传本质在居群水平通常是个量变的过程，它与种内其他非道地药材区别主要表现为居群内基因型频率的改变；在个体水平表现为微效多基因控制的数量遗传，或是微效多基因和主基因联合控制的数量性状。

After PCR primer for FMDV and multiple PCR primers for type Asia I virus and differention of Cathay and PanAsia topotypes of FMD type O viruses were designed,the optimal conditions of RT-PCR and multiple RT-PCR were determined by comparing the products of PCR.

在大量比较国内外口蹄疫病毒流行毒株序列的基础上自行设计了检测FMDV通用型引物及FMDVAsiaI型、O型口蹄疫病毒不同基因型即：中国型与泛亚株的多重PCR引物。

We sequenced the whole genome of the proband, identified all potential functional variants in genes likely to be related to the disease, and genotyped these variants in the affected family members.

我们对该病的先证者进行全基因测序，鉴定所有可能与该病相关的功能性变异基因，并确定在受累家族成员中这些变异基因的基因型。

So MyoD gene family is very important to the vertebrate in differentiation ofmuscle cell and developmental maturation of skeletal system, and that the analysis ofMyoD gene family has theoretical and practical significance in poultry′s breeding.

同时结合测定的生产资料，分析了MyoG基因5′调控区和MyoD基因各多态位点产生的不同基因型之间在屠宰性状和肉质性状的差异。从而找出这两个基因和与屠宰性状和肉质性状之间的相关性，为鸡的标记辅助育种工作提供参考依据。

The PPO activity of genotype 2AL/2DL,2AL/2DH,2AH/2DL,2AH/2DH get higher one by one for one degree40-60AU/min·gand their activity level is low medium,medium,medium high,high medium,respectively;and they make activity decrease 30.1%,decrease 7.8%, increase 8.3%,increase 30.0%,respectively.8.2AL and 2DL gene make kernel PPO activity decrease 25%and 10%, respectively.2AH and 2DH gene make kernel PPO activity increase around 17%.2AL, 2DL,2AH,2DH keep PPO activity at low,medium,medium high,medium high level, respectively.

四种基因型2AL/2DL、2AL/2DH、2AH/2DL、2AH/2DH的籽粒PPO活性逐渐升高一个档次(40-60AU/min·g)，且其活性分别处于低偏中、中等、中偏高、高偏中水平，可分别使活性降低30.1%、降低7.8%、升高8.3%、升高30.0%。8.2AL和2DL基因分别使籽粒PPO活性降低25%和10%。2AH和2DH基因均可使籽粒PPO活性增加17%左右。2AL、2DL、2AH、2DH基因分别使籽粒PPO活性维持在低、中等、中高、中高水平。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。