基因型

Methods: the experimental condition for preparing the gene chips was studied and 7 fragments were screened from GenBank as probes dotted on chips to make HBV genotyping gene chips, many examinatorial experiments were done to evaluate the quality of this gene chip, and to analysis its detection characteristics.

对大量HBV各基因型序列对比分析后，设计7支探针，用芯片点样机点样于经预处理的玻片上制成HBV基因型检测芯片；对芯片检测的实验条件进行研究探索；进行芯片质量性能的考察实验，分析其检测特性。

The inheritance of poly-eggs was investigated by crossing and reciprocally crossing APIV with monoembryonic rice variety IR36,Minghui No.77 and Longtepu B,respectively.It was suggested that the production of poly-eggs is probably controlled by gametophytic genotypes,rather than sporophytic ones.

通过多胚水稻品系APⅣ与单胚水稻品种IR36、明恢77和龙特浦B正反杂交，研究APⅣ的多卵遗传行为，表明APⅣ的多卵性状可能不是由孢子体基因型决定的，而是由雄配子体基因型决定，属配子体遗传的范畴。

The genotypic distribution based on the HV available, in accordance with classification based on the sequence analysis of the entire genome.

提示在基因型已确定情况下，对同一基因型内相近分离株，可以根据高变区的比较来进行亚型的分类。

Methods 209 patients with CHD and 101 controls were selected. MTHFR genetic C677T polymorphism was determined by PCR-RFLP, and CBS T833C polymorphism by ARMS method. Plasma homocysteine levels were detected with HPLC. Results The frequencies of MTHFR T homogenetic type and heterogenetic type (27.8%and 45.4%) in case group were higher than those in normal group(22.8%and 34.6%).

对209例冠心病患者和101例健康对照者，采用多聚酶链反应-限制性内切酶片段长度多态性技术检测MTHFRC677T基因型，用扩增阻滞突变体系法检测CBS基因T833C多态性，采用高压液相色谱法测定同型半胱氨酸血浆水平。

It might be due to the increase during pregnancy in folate uptake capacity via altered hormone regulation. In pregnant women without folate supplement, levels of folate metabolites varied, which was associated with genotypes of MTHFR, MTR, MTRR, ALDH1L1, and AMT. The 5FoTHF/5,10CHTHF ratio is relatively low in pregnant women carrying heterozygotic SNPs of MTHFR, MTRR, ALDH1L1 genes. It may be as a maker for evaluating genetic effects on folate maintenance.

在未服用叶酸的受试者中，叶酸代谢途径的MTHFR、MTR、MTRR、ALDH1L1、AMT等基因型对於叶酸各代谢物的浓度均有显著性影响，而5FoTHF/5，10CHTHF在MTHFR、MTRR、ALDH1L1的5个SNP中发现具变异型配子的受试者有比值较低的趋势，可以作为单纯评估个体受到来自於基因型层面影响的依据。

In addition, the genotype-phenotype and race relationship study is growing more and more noticeable because HCM is not only a genetically and phenotypically heterogeneous disease but also a disease has racial difference. However, most of the knowledge is derived from studies in Caucasian patients, little is known in Chinese patients with HCM.

国外学者对上述基因突变的分子遗传学和临床研究显示这些突变与肥厚型心肌病的临床表现密切相关，因而研究其基因型及临床表型关系具有重要的诊断意义。

HEV-RNA was detected by nested PCR. And positive PCR products were sequenced and analyzed phylogenetically with neighbour-joining method.

对深圳市采集的80份猪粪便标本和6份戊型肝炎病人血清，提取病毒RNA，然后进行PCR检测，阳性时对扩增片段进行基因序列测定，并进行基因型分析。

All statistical analysis was performed using SPSS statistical software and UNPHASED Software. The genotype and allele frequency distribution were compared with χ 2 analysis, haplotype analysis was performed using UNPHASED Software., and multivariate logistic regression were used to evaluate the OR value.

统计分析使用 SPSS11.5 和 UNPHASE 软件进行，基因型分布比较用χ2 检验，通过 logistic 回归模型计算各基因型对抑郁症的相对危险度，单倍体分析用 UNPHASE 软件实现。

Univariate analysis of variance components indicated that FUT1 gene and breeds influence significantly total number born, parities influence total number born unsignificantly. FUT1 gene,breeds and parities influence unsignificantly the litter size alive.

用方差分析方法分析FUT1基因型、品种和胎次与产仔性状之间的关系，基因型和品种对猪的总产仔数的影响显著，胎次对总产仔数影响不显著。

A nested-RT-PCR technique was established for SCYLV detection from individual viruliferous aphids and plant in early stage of infection, about 80% adult aphids feeding on sugarcane plants with yellow leaf symptom for over 2 weeks showed SCYLV positive by this technique.

但广州甘蔗研究所品种基地源自美国运河点育种场的CP93-1319病株上的病毒与已报道的三个基因型存在较大差异，可能代表了SCYLV的一个新的基因型。本文首次证实华南地区甘蔗上普遍发生的甘蔗绵蚜为SCYLV传毒媒介。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。