基因型

We aim to screen and identify the key potential trans-factors during hemoglobin switch. We firstly analyzed differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal umbilical cord blood, adult bone marrow, and bone marrow of a heterocellular hereditary persistence of fetal hemoglobin patient. We identified ZF (HCF-binding transcription factor, Zhangfei) and SH3GLB1(SH3-domain GRB2-like endophilin B1) that had differential expression in the above three cultures. Furthermore, we confirmed the different expression of the above two genes by quantitative real-time PCR.

为鉴别影响珠蛋白基因表达和开关的新的重要调节因子，我们首先分析了人脐带血、正常成人骨髓和异细胞型胎儿血红蛋白持续存在综合症患者骨髓细胞红系诱导培养物中基因表达的差异，发现ZF(HCF-binding transcription factor，Zhangfei)和SH3GLB1（SH3-domain GRB2-like endophilin B1）在这三种不同来源的红细胞内具有显著的表达差异，并通过实时定量PCR方法验证了差异的真实性。

BACKGROUND: Retinitis pigmentosa is a group of commonest genetic blindness-inducing eyeground diseases, which have relative great heterogenicity in both heredity and phenotype. Pierce et al discovered a new retinal photoreceptor cell specific gene-RP1 in 1999, and also found in their following research that the mutation of this gene can induce autosomal dominant RP .

背景：视网膜色素变性(retinitis pigmentosa，RP)是一组最常见的遗传性致盲眼底病，在遗传和表型上均具有较大的异质性。1999年Pierce等发现了一个新的视网膜感光细胞特异基因--RP1，之后的研究发现该基因的突变可导致常染色体显性遗传RP(autosomal dominant RP，adRP)。

So, to study the mechanism of phynotype transition is an important aspect to provent hyperplasic vascular desease.In 1998, Gill's study showed the cellular repressor of E1A-stimulated genes could promote cell differentiation and maturity. Also, CREG might effect on the maintenance of cellular differentiation status.

因此，对VSMC表型转化机制的研究是增生性血管病防治研究的一个重要方向。1998年，Gill等研究提示，人E1A激活基因阻遏子基因(cellular repressor of E1A-stimulated genes， CREG)可以诱导细胞的分化和成熟，并参与成熟组织中细胞分化状态的维持。

At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达，tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常，心脏畸形随着注射剂量的增加而出现比较一致的表型，而c-MO组胚胎没有出现明显的畸形；tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度，验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性；tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等，并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

Furthermore, a genetic screen was carried out to identify modifiers of the phenotypes associated with the constitutive expression of a dominant negative kuz transgene in developing imaginal discs.

另外，根据能否改变突变型kuz基因在器官芽中过量表达引起的表型，我们对覆盖了果蝇25％必须基因的2383个P因子插入突变进行了遗传筛选。

Our work is to screen alkali-tolerance deficient mutants from a T-DNA insertional library of C.

本论文主要目的在于获得耐碱缺陷型盾壳霉，从中克隆耐碱相关的基因，为培育耐碱作物积累基因材料。

Isogenic research on aroma gene showed that aroma gene between Luxianghui 2 and 6 aromatic CMS lines was isogenic genes.

香味基因的等位性研究结果，泸香恢2号与6个香型不育系的香味基因是等位的。

Methods HLA-A locus was typed with polymerase chain reaction-sequence specific primer technique for 259 longevous people (aged 90～105) and 214 healthy,randomly sampled subjects (aged 23～70) who live in the same area and comparative analyses were carried out among relevant alleles between the two groups.

方法应用聚合酶链式反应-序列特异引物对广西巴马和东兰两县259名90岁以上壮族长寿老人(90~105岁)和214名当地健康、随机抽样的壮族成年人(23~70岁)进行HLA-A座位的基因分型并进行相应等位基因频率比较。

We describe morphologic and immunohistochemical characteristics of a melanotic Xp11 translocation renal cancer occurring in an 18-year-old girl and perform molecular genetic studies to analyze its genetic alterations and related melanogenetic activities.

在此，我们描述一例黑色素性Xp11基因易位肾癌的形态特征及免疫表型，并通过分子遗传学研究分析本例的遗传学改变以及相关黑色素基因的表达调控情况。此患者女性，18岁。

Two materials of normal-spiked multispikelet lines-Branch 1 and'88〓185'were monosomic analysised with the Chinese Spring monosomicseries.As results,some new good genes which could be utilized atwheat breeding were located on certain chromosomes.

对具普通穗型的两个普通小麦多小穗品系——分枝1号和&88〓185&进行单体分析的结果，定位了一些在育种中可利用的新的优良基因，并对这些基因在育种中的利用方式进行了分析。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。