基因内区

In order to clarify the taxonomic status of Dirofilaria sp. of Ailurus fulgens,the second internal transcribed spacer (ITS-2) of rDNA of Dirofilaria immitis from dogs in Ya'an in Sichuan Pro-vince and of Dirofilaria sp.from Ailurus fulgens in Chengdu and Chongqing were sequenced and compared.

为探讨采自小熊猫的恶丝虫虫种的分类地位，测定了四川雅安地区犬源犬恶丝虫和成都及重庆两地小熊猫源恶丝虫的核糖体第二内转录间隔区（ITS-2）基因序列并进行了比对分析。

Methods With polymerase chain reaction-single-strand conformation polymorphism combining argentation and glue retrieval, DNA sequencing, and restriction fragment length polymorphism, the SNP of the 50 exons in all the coding regions of ABCA1 gene was detected in 112 patients with established diagnosis of coronary heart disease.

通过聚合酶链反应－单链构象多态性分析结合银染后胶回收、DNA测序和限制性内切酶酶切分析方法检测112例明确冠心病诊断患者的ABCA1基因全部编码区50个外显子的SNP。

Gene lies in nucleic acid sequence of endogenesis transcriptino alternation district between 16 Ss-23 S rDNA, reagent box includes 10 x PCR buffer, 10 X dNTP, 10 x introductor Cff A1/introductor CffA2 and Tag enzyme.

基因位于16S－23S rDNA间的内源转录间隔区的核酸序列，试剂盒包括10×PCR缓冲液、10×dNTP、10×引物CffA1/引物CffA2和Tag酶。

A represents the labeled fragments as position control; H1 represents the human H1gene as positive control; NEG represents the 431 bp fragment of bacterial 16s RNA as negative control; X,Y,Z,R,T,U represent the uncharacterized genes of SARS virus, which functions are unknown, the number under the parenthesis represents the position of each gene on the virus genome.

其中A表示利用已经标记好的片段作为坐标点；H1为人H1基因cDNA片段，作为芯片杂交的阳性点；NEG表示细菌16sRNA的一段长431bp片段作为芯片杂交的阴性点；X，Y，Z，R，T，U为推测的蛋白编码区，其功能还不清楚，括号内数字表示在基因组上的相对位置。

On the other hand, for ADRBK2 gene, although 15 novel single nucleotide variants were found in exonic and flanking intronic regions, the frequency of variants were very low, and only one rare missense variant and two silent variants were found in coding region.

对于ADRBK2基因，在外显子及外显子与内含子交接区域内共发现了15个新的变异，其中在编码区发现了一个罕见的错义变异Kps208S叫和两个同义变异。

A type has 7 point mutations(5 samples). B type has 6 point mutations (3 samples), C type has 4 point mutations (3 samples) and D type has 2 point mutations(1sample). Acid amino alignment shows that the most mutations of gastric cancer are mis-sense mutations, few ones are synonymous mutations. Most of the mutations occurred in regulating domain down to the HMG-box,only 2 sites in HMG-box.The mutations have same sites in middle and late stage samples. The results revealed that SOX4 mutations are associated with gastric cancer,which is possible a late accident in gastric cancer.

通过对相应的氨基酸序列的比对发现，发生在中晚期胃癌中的突变多为错义突变，同义突变不多，且突变大多发生在HMG盒下游编码区，在HMG盒内的突变只有两处，中期胃癌与晚期胃癌的突变有相同之处，但SOX4基因的突变位点随肿瘤的演进有增加的趋势。

Based on the conserved region nucleotide sequences of four viruses (Alfalfa Mosaic Virus, ALMV; Cucumber mosaic virus, CMV; Cucumber mosaic virus-satellite, CMV-sat; Potato virus Y, PVY) and one viroid (Potato spindle tuber viroid, PSTVd) which could be pathogenic on potato world and one inner control(18S rRNA), specific oligonucleotide probes and primers were designed and spotting for hybridization detection.

利用芯片点样仪将5种侵染马铃薯的病毒/类病毒(苜蓿花叶病毒、黄瓜花叶病毒、黄瓜花叶病毒-卫星病毒、马铃薯病毒Y、马铃薯块茎纺锤状类病毒)的保守区寡核苷酸(Oligonucleotide，oligo)探针和PCR探针点样于玻片，并以植物18S rRNA作为内参照制成基因芯片。

The protein interaction domains was delineated at the N-terminal 50-amino-acid fragment of HCV core protein and the C-terminus of p53. Confocal analysis also revealed that these two proteins colocalize in subnuclear granules and peri-nuclear region. Transfection experiments using a p53-responsive reporter plasmid in HuH-7, Hep3B, HepG2 and H1299 demostrated that full-length HCV core protein could elicit a positive or negative effect on the p53-mediated transcriptional activation depending on the concentration of the HCV core protein.

更且利用p53蛋白C端删除55-95个胺基酸之质体与含p53蛋白结合区之报导基因和表现全长HCV核心蛋白三者之质体於H1299细胞株进行共同转染时，与全长p53蛋白比较，发现HCV核心从增强全长p53蛋白之转活化能力转为抑制p53蛋白缺乏C端55-95个胺基酸之转活化能力，因实验室已有结果证实活体外及活体内缺乏C端55个及75个胺基酸之p53蛋白无法与HCV核心蛋白进行结合(Kao， unpublished data)。

Based on rice protein kinase sequences obtained from the database, kinases of functional representative and intron-free within gene were selected, 32 pairs primers were designed in this experiment; coding sequences of these kinases were obtained by PCR and cloned into yeast expression vector pEGH. GST-fusion proteins were expressed in Saccharomyces cerevisiae Y258, and their autophosphorylation activities were detected furthermore.

本实验利用公布的水稻激酶序列，选取功能有代表性并且其基因内部没有内元的32个序列，通过PCR扩增获得水稻激酶区的编码序列，克隆于酵母表达载体pEGH上，在酿酒酵母Y258菌株中表达融合蛋白质，并进行了自我磷酸化活性分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。