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It is the differentia that three kinds of genotypes frequency of panmixia parts of common population are constant in every generation,but variable in selfing part.

不同的是普通群体随机交配部分的3种基因型频率在各世代是固定的量,自交部分的3种基因型频率是变量。

The differentiation coefficient is 0.07 between three inbreed families respectively. The highest level of genetic homozygosity is in families Ⅱ at 18, but genetic variation is a little difference between inbreeding pig and mice. However, this study revealed the pattern of genetic variation at microsatellite loci in the 3 inbred families of WZSP with one of the most important findings to be the high level of unexpected heterozygosity at some loci, e.g. Sw874 and Sw936 that could be part of the unique genetics of three families. A hypothesis is that certain functional genes linked with such microsatellite loci may be involved in the maintenance of viability of these inbred families of WZSP remains for further investigation.

随近交代数的推进,每个星座上等位基因数越来越少,基因的纯合度越来越高,但WZSP近交系微卫星等位基因的纯合度有一定的限度,与近交鼠有所不同;在近交系3个家系各个世代中有少数几个基因座,如Sw874和Sw936等一直处于高度杂合状态,这可能与WZSP近交家系的种质特异性有关,推测与这些基因座处于同一连锁群的某些功能基因在维持极高近交水平下WZSP的基本生存能力中起着关键作用。3个近交群体间的分化系数在0.07以上,已各自成为独立家系;3个家系的近交程度不完全一致,基因的纯合度亦不同,Ⅱ系最高、14个微卫星座中7个纯合,其次为Ⅰ系和Ⅲ系。

When population are in noneequilibrium, if we know the genotype frequency of one generation of common population, the genotype frequency of the next or any generation can be calculated on the basis of the panmixia ratio and gene frequency directly.

群体在非平衡条件下,如果已知普通群体某一世代的基因型频率,根据随机交配率、基因频率可直接推算下一代或任一世代的基因型频率。

Through selection we obtained transgenic restorer line T773-1 with good agronomic traits and obvious resistance to striped stem borer and herbicide.

3转cry1Ab和bar基因的水稻恢复系T773-1和转Xa21基因的恢复系T773-2进行杂交、自交,对自交世代单株进行cry1Ab、bar和Xa21基因的分子标记辅助选择,获得了带有三基因的恢复系T773,田间种植对水稻白叶枯病、鳞翅目害虫、除草剂具有很好的抗性。

Deduced by the proportion of typical sterile plants and normal fertile plants in segregative generations derived from WA zhenqiuA/6078 that:I gene could inhibit the expression of Rf gene completely by the heterozygosity of 1 pair of Rf in genotype;and only reduce the expression of Rf genes with two heterozygous Rf genes in genotype ;but it would never inhibit the expression of Rf genes if the genotype included 3 pairs of heterozygous Rf genes. When Rf gene was homozygous,the / gene could not inhibit the expression of it.

根据认叭真秋刀6078各衍生分离世代中典型不育株和正常可育株所占比例推断:基因型中仅包含1对杂合的Rf基因时,I对Rf的表达起完全抑制作用;基因型中包含2对杂合的Rf基因时,I仅对Rf的表达起削弱作用;当基因型中包含3对杂合的对基因时,I对Rf的表达不起抑制作用;I对纯合位点的Rf的表达不起抑制作用。

Assume that individuals with the same major gene genotype in a segregating generation are normally distributed due to the variations of polygenes and environment, and individuals with different major gene genotypes follow a mixture of normals with different means and the same variance.

假定分离世代中的同一主基因基因型因多基因和环境的变异而呈正态分布,不同主基因基因型则为具不同平均数和相同方差的混合正态分布。

In order to improve the resistance to bacterial blight of high quality rice male sterile line Jinshan A , in this study, the Jinshan B and IRBB21 that carried gene Xa21 were used as parents to make backcrosses, and both foreground selection and background selection were carried out by means of marker assisted selection in the backcrosses.

本研究以IRBB21为Xa21基因的供体亲本,以金山B为轮回亲本进行回交,并应用分子标记辅助选择技术对回交各世代同时进行前景选择和背景选择,得到如下结果:(1)利用Xa21基因内的STS标记MXA21对各回交世代进行前景选择,得到了44个带有Xa21基因的BC_1单株和40个带有Xa21基因的BC_2单株。

On the basis of a simplified assumption that the target loci are introgressed and the genetic background are independent,the probability of selecting individuals with desired genotypes in each generation is approximately estimated combining analytical approach and simulation according to the graphic genotypes of backcrossing parents.The minimal sample size required to obtain at least one desired individual with a given probability is estimated.

在假定所需转移的目标基因座与遗传背景之间为相互独立的简化假设下,可以通过将解析方法与基于回交亲本图示基因型的模拟方法相结合,近似地估计出在每一世代中选到所需基因型的概率,进而估算出在一定概率水平下至少获得一个符合要求的个体所需的最小样本容量。

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状,即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况,本研究从筛选玉米转基因工程所需要的优良受体入手,采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法,以及现代分子标记定位主效QTL分析方法,对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究,取得以下主要研究结果:(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合,在相同培养条件下,对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定,发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数,但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系,有的基因型,如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异;有的基因型,如自交系综31,仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599,但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距,仅为19-599和18-599的1/3左右;有的基因型,如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低,平均仅在3%左右,而且胚性愈伤组织绿苗发生数表现也很差,基本上没有分化成苗。

According to molecular mechanism of gene mutation, no matter what transvertion or transition happens, several replications are needed.

根据基因突变的分子机制,无论是碱基的转换或颠换,都需经过几次的复制,即经过几个世代才能完或,这也是在后期世代才出现高油酸变异的原因。

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