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The fungus were firstly screened in Congo red plate for cellulase, and then cultured in residues of chinaberry as only carbohydrate supply.

采用纤维素刚果红平板培养基,对采自陕西秦岭山区的土样和腐烂枝干进行初筛,再以川楝残渣为惟一碳源进行摇瓶培养复筛,最后对获得的高纤维素酶活力菌株进行形态学和分子生物学鉴定。

The dechlorination of pentachlorophenol by anaerobic granular sludge was studied in serum bottles.

在间歇培养血清瓶中,研究了厌氧颗粒污泥对五氯酚脱氯过程中的影响因素。

B order to make full use of Ta1, it is essential to improve the investigation to industrial production scale by drawing assistance from high cell density fermentation. Pilot test of tube culture and flask shaking culture were done to get optimized culture conditions. Finally, we reached the goal of high cell density fermentation and high level expression of recombinant human Ta1 E.

在经过试管以及三角摇瓶水平对培养条件进行摸索后,我们成功地实现了该工程菌的高密度发酵,3乙菌液获得湿菌体工4口丁蛋白表达水平与试管水平相当。

In the serum bottle fermentation,potassium ferricyanide and dithiothreitol which did not have inhibition on fermentation,were selected as the oxidizing agent and reducing agent respectively.

在血清瓶发酵培养过程中,筛选出对发酵过程无抑制作用的氧化剂铁氰化钾和还原剂二硫苏糖醇作为发酵体系的氧化还原电位调节剂。

Results showed: under the condition of liquid culture, 29℃/20℃ and 8 hours light time per day, the induction rate and the fresh weight of microcorm reached the highest. If the fresh weight of microcorm is more than 0.8g, the mean sprouting rate of the 4 genotypes was more than 80%.

试验结果表明:在以液体培养方式、温度为29℃(1ight)/20℃和光照时间为8h/d时的条件下,试管球茎的诱导效率达到95%以上,单瓶试管球茎的最大鲜重和平均鲜重也达到4.5g和2.0g以上。

A high-yielding mutant Rhizopus arrhizus R4 producing γ-linolenic acid was successfully obtained when the original strain Rhizopus arrhizus R0 was treated by UV,and by the screening methods of resisting maleic hydrazide.

以少根根霉R0为出发菌株,利用紫外诱变结合失水苹果酰肼筛选的方法,选育出突变株R4,摇瓶培养菌体油脂含量35.55%,其中GLA占油脂的12.5%,比原始菌株油脂含量提高了93.94%,GLA提高了276.5%。

After seven days of training if bottles, fermentation liquid filtration, and has been hypha rough enzyme fluid.

经过7d的摇瓶培养,过滤发酵液,得到菌丝体和粗酶液。

The mutant was cultured on the rotating shaking bed at the speed of 200 r/min under 25℃for 72 hours,with inoculum size 8% and medium volume 40 mL/500 mL.

菌株于25℃在200 r/min的旋转式摇床上振荡培养72 h,接种量为8%,装液量为40 mL/500 mL三角瓶。

At the condition of shake flask fermentation,the metabolic characters of Bacillus pumilus in oleic acid were studied using proteose as the main nitro source and glucose as the carbon source.

一株具有转化油酸羟基化的微生物Bacillus pum ilus BD-12(记作BP BD-12),在摇瓶条件下分批深层培养,研究了菌株在以胰蛋白胨为主要氮源、葡萄糖为基本碳源的条件下对油酸的代谢特性,并对转化OA生成羟基脂肪酸的规律及产物进行了分析测定。

In phase one, the suspended liquid from semicontinuous slurry microcosms was used as an inoculum in CSTR. In phase two, the effluent of column experiment was used as an inoculum in CSTR. The purpose of two phases is to find out the correlation between environment factors and toluene-utilizing bacteria community for each phase, and to compare with the correlation between phase one and phase two.

混合连续流实验依菌种来源不同分为两阶段进行,第一阶段利用半连续培养泥浆法血清瓶中的悬浮菌液作为菌种来源;第二阶段则是利用管柱试验之出流液作为菌种来源,比较及观察两阶段反应器内的环境因子与甲苯分解菌社会结构的关联性。

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