培养瓶

Objective : To optimize the culture condition of adventitious roots of Panax ginseng .

中文摘要：目的：对人参不定根的摇瓶培养条件进行系统的优化。

The process index of aerobiosis exposural pool treatment method can be selected by aerobiosis vibration bottle.

可以通过好氧振荡瓶培养法来选择好氧曝气池处理法的工艺参数。

Temporarily catheterization pulls out inside 24 H ureter, if undertake uric education or acute retention of urine wait,adopt temporarily catheterization; leaves buy catheterization to be in clinical on common, ureter needs to leave above of buy 24 H, urethral mouth is daily want clean disinfection 2 times, daily change 1 times; keeps bag of collect make water or drainage bottle the person that buy catheter exceeds 7d, change every week ureter 1, with 0 · normally according to the circumstance of fluid of eduction make water 9% physiological saline undertake bladder is rinsed. 2 common problems patient of a few males is opposite circumstance of the psychogenic disorder when 1 catheterization of 2 · nurse catheterization produces psychogenic disorder, often be flushed, next abdomen and perineal ministry muscle are nervous, shadow fugleman make water goes on wheels, impact of urgenter to the illness patient psychogenic disorder is not big, ache and bladder are bouffant the mood that held them, catheterization process is more successful. 2 · 2 see the phenomenon is cracked below road junction of female patient make water occasionally 20 old clinical nurse in the job, the female cracks a circumstance to see 4 below urethral mouth.

临时导尿在24 h内拔除尿管，如进行尿培养或急性尿潴留等采取临时导尿；留置导尿在临床上常见，尿管需留置24 h以上，尿道口每日要清洁消毒2次，每日更换集尿袋或引流瓶1次；留置导尿管超过7d者，每周更换尿管1次，根据排出尿液的情况通常以0·9%生理盐水进行膀胱冲洗。2常见新问题2·1导尿时心理障碍情况一些男性患者对护士导尿产生心理障碍，往往面红耳赤，下腹部及会阴部肌肉紧张，影响导尿顺利进行，对于病情较急的患者心理障碍影响不大，疼痛和膀胱鼓胀占据了他们的情绪，导尿过程较顺利。2·2偶见女性患者尿道口下裂现象20多年的临床护理工作中，女性尿道口下裂情况见4例。

The highest enzyme producer alkalophilic Bacillus sp SHY8-1 among the inductive strains was mutagenized several times, by adding enzyme production repression aegents into screen medium, combined with natural seperation and pressure selection, finally a CMCase-hyperproductive mutant SHY8-1-25 (partitally resistant to catabolite) was obtained, with the yield of CMCase raised to 13-16u/ml, maximally reached 18-20u/ml, while its parent strain 0.654u/ml.

对诱导型产酶菌-嗜碱芽孢杆菌SHY8-1进行了多次诱变改良，采用添加产酶阻遏剂的平板作为筛子，结合反复的自然分离、压力选择，最终成功地获得了高产变异株SHY8-1-25(已部分解除分解代谢阻遏)，产酶量由0.654u/ml提高到13-16u/ml，最高达到18-20u/ml，讨论了诱变机理策略，考察了碳、氮源等对产酶的影响，对培养基和摇瓶培养条件进行了优化。

When feeding with undecylenic acid by flask culture, weight of PHA and PHA content were 2.148 g/L, 51.8% respectively.

锥形瓶馈加十一烯酸培养实验，於培养40小时取样可得最高菌量、最高PHA累积量分别为4.14g/L、2.148g/L，PHA含量可提升至51.8%。

The production and characterization of endoglucanase from Streptomyces xylophagus KX6 was studied. Maximum endoglucanase yield of 0.538 IU/ml was achieved with medium pH8.0, containing CMC2Na 1.0% as carbon resource, soybean meal 1% as nitrogen resource, 2% inoculating volume, 30% 250 ml triangle flask bulk for medium volume at 40℃ 200r/min shaker for 48h.

实验中对其产酶的液态发酵条件进行了研究，碳源为1%羧甲基纤维素钠，氮源为1%豆粕粉，250ml三角瓶30 %装液量，接种量为2%，培养基初始pH为8.0，培养温度为40℃，200r/min培养48h后，发酵液中内切葡聚糖酶活达到0.538IU/ml。

Finally indicated, the best seed raise conditions of T11-1 and O7-7 is: 100mL seed culture mudium in 200 mL triangle bottle ,16 hours in shake bed;It′s best component fermentation medium is: peptone 5.0,yeast extract 3.0, beef paste 2.0,xylose 60.0,K_2HPO_4 2.0,MnSO_4·4H_2O 0.04,MgSO_4·7H_2O 0.20,FeSO_4·7H_2O 0.05;The best fermentation condition is: 150mL fermentation medium in 250mL bottle,vaccinates 10 %, 48 hours in shake bed under 46℃;The output of L-lactic acid using T11-1 and O7-7 from corncob respectively were 31.30g/L and 30.88g/L,and the conversion rate respectively were 52.17% and 51.47%.

结果表明，T11-1和O7-7的最佳种子培养条件为：在200mL三角瓶中于盛装100mL种子培养基，摇床培养16h；其最佳发酵培养基组分为：蛋白胨5.0，酵母浸粉3.0，牛肉膏2.0，木糖60.0，MnSO_4·4H_2O 0.04，MgSO_4·7H_2O 0.2，FeSO_4·7H_2O 0.05，K_2HPO_4 2.0；最佳发酵条件为：250mL输液瓶装载150mL发酵培养基，接种10%，在46℃条件下摇床发酵48h；利用T11-1和O7-7进行玉米芯L-乳酸发酵，其L-乳酸产量分别为：31.30g/L和30.88g/L，转化率分别为52.17%和51.47%。

It is very important to biological treatment of angiogenesis diseases such as hypoxic/ ischemic cerebral diseases and cerebral ischemia/ reperfusion.PC 12 cells were treated with cobalt chloride (125uM) for 0、1、 2、4、8、12、16 hours.

离体培养的PC12细胞长满瓶底后，加入终浓度125uM氯化钴的培养液，孵育0、1、2、4、8、12、16h，检测细胞培养上清液中LDH的漏出；并收集缺氧0、1、4、8、16h细胞，戊二醛固定，切片，电镜观察细胞超微结构的改变。

Through shake flask experiments,it was acquired that the best culture conditions:pH6.5,37℃,240rpm,40mL/300mL,and 2.5%of bacterium culture for 12h.

通过摇瓶发酵试验，确定其最佳培养条件为：摇床37℃，240rpm，发酵培养基的起始pH为6.5，接种量为2.5%，装液量为40mL/300mL，培养32h。

Through the selection of different factors to develop, analyze and optimize the stage reached in shake flask culture conditions are as follows: K +, Mg2 +, P5 +, VB mycelium growth is necessary nutrients.

通过选定不同因子来培养，分析并得出摇瓶阶段优化培养条件如下：K+、Mg2+、P5+、VB是菌丝体生长所必需的营养成分。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。