培养瓶

In the above conditions, the maximum erythritol yield on fermented glucose was 29.6%.

最适培养条件是在摇瓶转速150 r/min的条件下于35 ℃培养4 d。

The results showed that the optimal carbon sourceand nitrogen source were amidulin,CaCO_3 and yeast.The optimization pH 7.0～8.0,cultural temperature:30—32℃,and germ Q6 was cultured for 48h inbroad-openning flask with 20% volume medium.

结果表明，在起始pH值7.0～8.0，接种量为5％以上，培养基装量为广口三角瓶体积的20％以下，培养温度30—32℃，振荡培养48h时Q6发酵产物的抑菌活性最强。

The biotechnology production efficiency of crocin II, which has anti-cancer activity, was improved in a solid-liquid two-step cell culture in which the callus cells obtained from the solid medium were transferred to the liquid medium.

为提高现代生物技术生产藏红花素的产率和效率，该研究采取固液两步培养法将在固体培养基中获得的藏红花愈伤组织转移到液体培养基中进行生物合成，结果显示生物反应器比摇瓶培养更有利于生物量的积累，也更有利于2号藏红花素的合成。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

The results from mixotrophic cultivations in shake flasks showed that light was necessary for mixotrophic growth of Synechocystis sp.

在摇瓶中对集胞藻 680 3利用有机碳源特性进行研究的结果表明，光照是集胞藻 680 3利用葡萄糖的必需条件，混合营养培养是集胞藻 680 3的最佳培养方式。

Under the studies on conditions of liquid fermentation of Poria cocos,the techniques of Erlenmeyer flask additional medium liquid fermentation and additional medium liquid fermentation in fermentor were studied in this paper.

本文在茯苓摇瓶液体发酵条件研究的基础上，进一步探讨了茯苓的摇瓶补料培养液体发酵和发酵罐补料液体发酵的发酵工艺。

4Mm harvested in preculture, is the best. In this form, Rhizopus oryzae R1021 produced most L-lactic acid (76. 62g/L), the pellet size in production medium in flask and fermentor are 1. 9mm and 2. 6mm, respectively, and its L-lactic acid productivity reached or exceeded that of the immobilized cell with polyurethane foam. The produced L-lactic acid and the yield of L-lactic acid based on the total glucose and the productivity are above 74g/L and 74% and 2. 69g/L h, respectively, in 7 batches repetitive fermentation in fermentor, and the size of pellets remain 2. 6mm～3. 4mm.

摇瓶种子培养获得的菌球直径为1.4mm时，这种大小的菌球产酸最高，为76.62g/L；研究表明以直径1.4mm的菌球为种子，在摇瓶发酵和发酵罐发酵中菌球大小分别为1.9mm和2.6mm；这种菌球的产酸水平最高，可以达到甚至超过聚氨酯泡沫载体固定化细胞发酵乳酸的水平；在反复7批次发酵罐发酵过程中，菌球的大小始终保持在2.6mm～3.4mm之间，L-乳酸产量、得率和生产能力分别保持在74g/L、74％和2.69g/L h以上。

In order to study the effect of carbon sources on the growth of suspension Roselle cell and it's nutrients assimilation, sucrose 、 glucose and soluble starch were used as carbon sources in flask culture of Roselle.

摘 要 采用了蔗糖、葡萄糖、可溶性淀粉3种不同碳源，考察了在摇瓶培养过程中，这3种碳源对维持悬浮培养过程中玫瑰茄细胞的生长及主要基质消耗的影响。

The adopted lactose concentration for induction, the time point of induction, the duration of induction and the dynamics of uricase expression were optimized and studied in detail by shake flask experiment.

通过摇瓶试验对诱导所采用的乳糖浓度，诱导时机和诱导持续时间进行了优化，并考察在乳糖诱导下的目的产物表达动力学，随后在5 L发酵罐上进行扩大化培养以验证摇瓶优化的结果，进一步将乳糖作为诱导剂应用于高密度发酵过程。

Inoculation number and medium volume tests suggested that the optimal inoculation number was 10%, and the optimal medium volume was 50mL/150mL with Erlenmeyer flask.

通过接种量、装液量试验，确认摇瓶培养茯苓菌丝体适宜的接种量为10％，装液量为50mL／150mL的三角瓶。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。