培养瓶

The optimal carbon source and nitrogen source were also determined as 20 g/L glucose, 15 g/L tryptone and 5 g/L beef extract, respectively.

经过对产酶条件的优化，确定最佳摇瓶产酶条件为：葡萄糖20 g/L，蛋白胨15 g/L，牛肉膏5 g/L，玉米浆15 g/L， KH2PO4 3 g/L， MgSO4·7H2O 0.5 g/L，初始pH 7.0，培养温度37℃，装液量20 mL/250 mL三角瓶，摇床转速200 r/min。

The steps for preparing insecticide include: first shaking flask for fermentation, adding amidon, soya bean cake powder, cotton cake powder, protease leather, yeast powder, maize milk, magnesium sulfate heptahydrate, monobasic potassium phosphate, dibasic potassium phosphate, calcium carbonate alpha-amylase and make-up water by ratio in culture medium with 100 unit volumes; secondarily filling 100ml culture mediums in triangular bottle, then seeding BTH-13 after disinfection and culturing them until the brood-cell pulls off under finite temperature; third adopting the selected culture medium and fermenting them in full-automatic fermentation tank, acidifying the fermentation liquor after the fermentation, adding the emulsifier, and directly performing spray-drying after the compression of the fermentation liquor to prepare the wettable powder.

制备杀虫剂的步骤是：首先是摇瓶发酵，在100单位体积的培养基中，按比例加入淀粉、豆饼粉、棉饼子粉、蛋白胨、酵母粉、玉米浆、七水硫酸镁、磷酸二氢钾、磷酸氢二钾、碳酸钙、及α-淀粉酶，补充水；其次是在三角瓶中装100ml培养基，灭菌后接种BTH-13，在一定温度培养至芽孢脱落；第三是采用筛选出来的培养基，在全自动发酵罐中进行发酵，发酵结束后发酵液酸化，加入乳化剂，酵液浓缩后直接喷雾干燥制备可湿性粉剂。

The results showed that the optimal compositions of medium were 15 sweet potato starch hydrolyte, 0.40 (NH4)2SO4, 0.03 KH2PO4, 0.044 ZnSO4-7H2O, 0.025 MgSO4-7H2O, and that the optimal culture temperature and rotation rate were 32 癈 and 220 r/min respectively.

摇瓶发酵最佳培养基：甘薯淀粉15，(NH_4)_2SO_4 0.4，KH_2PO_4 0.03，ZnSO_4·7H_2O 0.044，MgSO_4·7H_2O 0.025。最佳培养条件为32℃，摇床转速220 r／min，装液量100mL／500mL三角瓶，发酵16h后一次性添加CaCO_370g/L。

One hundred μL culture medium was taken with micro pipette tips from each conical flask at 0, 20, 40, 60 and 80 min for analysis activity of LDH.

在培养的第0、20、40、60和80min时从每个锥形瓶瓶中取100μL培养液分析乳酸脱氢酶活力。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

The effects of cultivation conditions such as temperature, intial pH, and broth volume on the bioacitivities of the biotransformation products of EGB by Hericium erinaceus in shaking flask were further investigated.

在前期优化培养基的基础上，本实验比较了摇瓶的温度、pH、装液量和发酵罐培养的通气量、转速和培养时间等不同培养条件对猴头菌转化EGB生物活性的影响。

Strains D1 and D2 have the highest lipolytic activity in culture supernatent, great amounts lipase produced by remainder strains was located on the outer membrane of the cell envelope and was not released into the culture medium .

在摇瓶发酵水平上进行产酶优化试验，确定D2产脂肪酶的最佳培养方案为：培养温度9℃，摇床转速180r/min，培养时间40h，以2216为基础培养基，5g/l牛肉膏做碳源，6g/l氯化铵做氮源，分别替代酵母膏和蛋白胨。

The optimal medium components were: sodium alginate 3 g/L,(NH4)2SO4 3 g/L, NaCl 20 g/L, KH2PO4 0.1 g/L, CaCl2 0.1 g/L; The optimal culture conditions were: 25 ml medium in 250 mL Erlenmeyer flask, inoculum's volume 3%, shaking speed of 150 r/min, initial pH 7.5, at 28°C for 24 h.

该菌株产酶的最佳培养基组成为：褐藻胶3 g/L、(NH4)2SO4 3 g/L、NaCl 20 g/L、KH2PO4 0.1 g/L、CaCl2 0.1 g/L；最佳培养条件为：250 mL三角烧瓶中装液量25 mL、接种量3%、摇瓶转速150 r/min、pH7.5、培养温度为28℃、培养时间为24 h。

With the single factor test and orthogonal test, the optimal culture media for HF3-04 strain producing agarase was decided as: agar 3‰, NH4NO31‰, Na2HPO4 0.5 mM, pH 8.0. The optimal fermentation conditions were inoculum size 3‰, 110ml medium in 250mL Erlenmeyer flask, rotation rate 150r∕min, culture time 48h,culture temperature 25℃.

通过正交试验确定其最佳培养基组成为琼胶3‰、硝酸铵1‰、磷酸氢二钠0.5mM、pH值8.0；该菌株的最佳培养条件为接种量1%、装液量为250ml三角瓶装入110ml发酵培养基、摇床转速150r∕min、培养温度25℃、培养时间48h。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。